Superfine bacterial nanocellulose produced by reverse mutations in the bcsC gene during adaptive breeding of Komagataeibacter oboediens

Taweecheep, Pornchanok; Naloka, Kallayanee; Matsutani, Minenosuke; Yakushi, Toshiharu; Matsushita, Kazunobu; Theeragool, Gunjana

doi:10.1016/j.carbpol.2019.115243

Cited by 12 publications

(7 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…They have some impairments in either bcsBI or bcsCI genes (Matsutani et al, 2015). A nonsense mutation in the bcsCI gene of Komagataeibacter oboediens MSKU3 has been reported to engender loss of cellulose productivity (Taweecheep et al, 2019). Therefore, we infer that the loss of cellulose productivity in the FNDCF1 strain is attributed to loss of a full functional set of the bcs operon.…”

Section: Discussionmentioning

confidence: 88%

Comparative Genome Analysis of Three Komagataeibacter Strains Used for Practical Production of Nata-de-Coco

Ishiya

Kosaka²,

Inaoka

et al. 2022

Front. Microbiol.

View full text Add to dashboard Cite

We determined the whole genome sequences of three bacterial strains, designated as FNDCR1, FNDCF1, and FNDCR2, isolated from a practical nata-de-coco producing bacterial culture. Only FNDCR1 and FNDCR2 strains had the ability to produce cellulose. The 16S rDNA sequence and phylogenetic analysis revealed that all strains belonged to the Komagataeibacter genus but belonged to a different clade within the genus. Comparative genomic analysis revealed cross-strain distribution of duplicated sequences in Komagataeibacter genomes. It is particularly interesting that FNDCR1 has many duplicated sequences within the genome independently of the phylogenetic clade, suggesting that these duplications might have been obtained specifically for this strain. Analysis of the cellulose biosynthesis operon of the three determined strain genomes indicated that several cellulose synthesis-related genes, which are present in FNDCR1 and FNDCR2, were lost in the FNDCF1 strain. These findings reveal important genetic insights into practical nata de coco-producing bacteria that can be used in food development. Furthermore, our results also shed light on the variation in their cellulose-producing abilities and illustrate why genetic traits are unstable for Komagataeibacter and Komagataeibacter-related acetic acid bacteria.

show abstract

Section: Discussionmentioning

confidence: 88%

Comparative Genome Analysis of Three Komagataeibacter Strains Used for Practical Production of Nata-de-Coco

Ishiya

Kosaka²,

Inaoka

et al. 2022

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

“…Thanks to recent advances in the characterization of biochemical mechanisms involved in bacterial nanocellulose biosynthesis, the future development of new genetically engineered bacterial nanocellulose-producing strains could be achieved. This could eventually lead to a reduction of production costs, an improvement of production yield, and biosynthesis of nanocellulose with new properties, suitable for broader range of technological applications [92,111]. Concerning genetic manipulation of nanostructure-producing microorganisms, Tan et al (2016) have reported a 2000-fold increase in electrical conductivity and diameter of nanowires filaments produced by model microorganism Geobacter sulfurreducens.…”

Section: Biochemistry Molecular Biology and Geneticsmentioning

confidence: 99%

Microbial Nanotechnology: Challenges and Prospects for Green Biocatalytic Synthesis of Nanoscale Materials for Sensoristic and Biomedical Applications

2019

View full text Add to dashboard Cite

Nanomaterials are increasingly being used in new products and devices with a great impact on different fields from sensoristics to biomedicine. Biosynthesis of nanomaterials by microorganisms is recently attracting interest as a new, exciting approach towards the development of ‘greener’ nanomanufacturing compared to traditional chemical and physical approaches. This review provides an insight about microbial biosynthesis of nanomaterials by bacteria, yeast, molds, and microalgae for the manufacturing of sensoristic devices and therapeutic/diagnostic applications. The last ten-year literature was selected, focusing on scientific works where aspects like biosynthesis features, characterization, and applications have been described. The knowledge, challenges, and potentiality of microbial-mediated biosynthesis was also described. Bacteria and microalgae are the main microorganism used for nanobiosynthesis, principally for biomedical applications. Some bacteria and microalgae have showed the ability to synthetize unique nanostructures: bacterial nanocellulose, exopolysaccharides, bacterial nanowires, and biomineralized nanoscale materials (magnetosomes, frustules, and coccoliths). Yeasts and molds are characterized by extracellular synthesis, advantageous for possible reuse of cell cultures and reduced purification processes of nanomaterials. The intrinsic variability of the microbiological systems requires a greater protocols standardization to obtain nanomaterials with increasingly uniform and reproducible chemical-physical characteristics. A deeper knowledge about biosynthetic pathways and the opportunities from genetic engineering are stimulating the research towards a breakthrough development of microbial-based nanosynthesis for the future scaling-up and possible industrial exploitation of these promising ‘nanofactories’.

show abstract

“…The cellulose negative phenotype may spontaneously appear as well as be reversed. It has often been reported that after several passages of Cel− forms in a static culture, it is possible to restore BNC biosynthesis ability (Matsutani et al 2015 ; Taweecheep et al 2019b ). Genomic DNA analyses of such cells (called ‘revertants’) have identified point mutations (insertions or deletions) in the bcsB and bcsC genes which caused the frameshifts restoring the BcsB or BcsC activity.…”

Section: Introductionmentioning

confidence: 99%

“…Genomic DNA analyses of such cells (called ‘revertants’) have identified point mutations (insertions or deletions) in the bcsB and bcsC genes which caused the frameshifts restoring the BcsB or BcsC activity. It is interesting that the point mutations in bcsCI not only resulted in the restoration of the BNC biosynthesis capability but also caused up to 6.5-fold increase in BNC yield (Taweecheep et al 2019b ). Moreover, the same study has shown that a single mutation in the bcsCI gene may not only improve BNC biosynthesis but also lead to changes in its structure and properties.…”

Section: Introductionmentioning

confidence: 99%

“…BNC fibril diameter size: 70.52 nm; density: 0.43 ± 0.05 g cm −3 . Mechanical properties of BNC; tensile strength: 73.94 ± 16.94 MPa, Young’s modulus: 5.83 ± 0.69 (Taweecheep et al 2019a , b ) E3 (parental strain for revertants) b A single nucleotide (T) insertion at the 2135th position of bcsCI Frameshift. Gene disruption by stop codon Repeated static cultivation of MSKU 3 strain for 63 passages (YPGD1A medium containing increasing concentration of ethanol) No BNC production.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Towards control of cellulose biosynthesis by Komagataeibacter using systems-level and strain engineering strategies: current progress and perspectives

Ryngajłło

Jędrzejczak-Krzepkowska

Kubiak

et al. 2020

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

The strains of the Komagataeibacter genus have been shown to be the most efficient bacterial nanocellulose producers. Although exploited for many decades, the studies of these species focused mainly on the optimisation of cellulose synthesis process through modification of culturing conditions in the industrially relevant settings. Molecular physiology of Komagataeibacter was poorly understood and only a few studies explored genetic engineering as a strategy for strain improvement. Only since recently the systemic information of the Komagataeibacter species has been accumulating in the form of omics datasets representing sequenced genomes, transcriptomes, proteomes and metabolomes. Genetic analyses of the mutants generated in the untargeted strain modification studies have drawn attention to other important proteins, beyond those of the core catalytic machinery of the cellulose synthase complex. Recently, modern molecular and synthetic biology tools have been developed which showed the potential for improving targeted strain engineering. Taking the advantage of the gathered knowledge should allow for better understanding of the genotype-phenotype relationship which is necessary for robust modelling of metabolism as well as selection and testing of new molecular engineering targets. In this review, we discuss the current progress in the area of Komagataeibacter systems biology and its impact on the research aimed at scaled-up cellulose synthesis as well as BNC functionalisation. Key points • The accumulated omics datasets advanced the systemic understanding of Komagataeibacter physiology at the molecular level. • Untargeted and targeted strain modification approaches have been applied to improve nanocellulose yield and properties. • The development of modern molecular and synthetic biology tools presents a potential for enhancing targeted strain engineering. • The accumulating omic information should improve modelling of Komagataeibacter's metabolism as well as selection and testing of new molecular engineering targets.

show abstract

Superfine bacterial nanocellulose produced by reverse mutations in the bcsC gene during adaptive breeding of Komagataeibacter oboediens

Cited by 12 publications

References 44 publications

Comparative Genome Analysis of Three Komagataeibacter Strains Used for Practical Production of Nata-de-Coco

Comparative Genome Analysis of Three Komagataeibacter Strains Used for Practical Production of Nata-de-Coco

Microbial Nanotechnology: Challenges and Prospects for Green Biocatalytic Synthesis of Nanoscale Materials for Sensoristic and Biomedical Applications

Towards control of cellulose biosynthesis by Komagataeibacter using systems-level and strain engineering strategies: current progress and perspectives

Contact Info

Product

Resources

About