Superfamily 2 helicases

Byrd, Alicia K.; Raney, Kevin D.

doi:10.2741/4038

Cited by 115 publications

(120 citation statements)

References 168 publications

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“…However, little is known about the role of VEGF-A in the endothelial-like differentiation of iDCs. VEGF, as an important angiogenesis regulatory factor, can significantly stimulate tumor angiogenesis [44,45]. Its characterized receptors are the tyrosine kinases, VEGF receptor 1 (VEGFR1), and VEGF receptor 2 (VEGFR2).…”

Section: Discussionmentioning

confidence: 99%

MAPK/ERK1/2 signaling mediates endothelial-like differentiation of immature DCs in the microenvironment of esophageal squamous cell carcinoma

Lü

Zhao

Liu

et al. 2010

Cell. Mol. Life Sci.

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Endothelial-like differentiation of dendritic cells (DCs) is a new phenomenon, and the mechanism is still elusive. Here, we show that the tumor microenvironment derived from the human esophageal squamous cell carcinoma (ESCC) cell line EC9706 can induce immature DCs (iDCs) differentiate toward endothelial cells, and become endothelial-like cells, but it has no obvious influence on mature DCs. During the course of endothelial-like differentiation of iDCs, a sustained activation of mitogen-activated protein kinase/extracelluar signal-regulated kinase1/2 (MAPK/ERK1/2) and cAMP response element-binding protein (CREB) was detected. Incubation of iDCs with MEK phosphorylation inhibitor PD98059 blocked the MAPK/ERK1/2 and CREB phosphorylation as well as the endothelial-like differentiation of iDCs. Inhibition of vascular endothelial growth factor-A (VEGF-A) in the microenvironment with its antibody blocked the endothelial-like differentiation and the phosphorylation of MAPK/ERK1/2 and CREB. These data suggest that MAPK/ERK1/2 signaling pathway activated by VEGF-A could mediate endothelial-like differentiation of iDCs in the ESCC microenvironment.

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Section: Discussionmentioning

confidence: 99%

MAPK/ERK1/2 signaling mediates endothelial-like differentiation of immature DCs in the microenvironment of esophageal squamous cell carcinoma

Lü

Zhao

Liu

et al. 2010

Cell. Mol. Life Sci.

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“…[1][2][3][4]. For many helicases, translocation along single-stranded DNA (ssDNA) 2 is related to their ability to melt dsDNA.…”

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confidence: 99%

A Parallel Quadruplex DNA Is Bound Tightly but Unfolded Slowly by Pif1 Helicase

Byrd

Raney

2015

Journal of Biological Chemistry

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Background: Some G-rich sequences fold into intramolecular quadruplex structures. Pif1 helicase reduces genomic instability by unfolding quadruplex DNA. Results: Pif1 unfolds a parallel quadruplex structure slowly relative to unwinding of duplex DNA. Conclusion:Comparison of duplex unwinding to quadruplex unfolding is complicated by distinct kinetic mechanisms. Significance: A highly stable, intramolecular quadruplex is a formidable obstacle but can be overcome by Pif1 helicase.

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“…Ensemble biochemical analyses have contributed considerably to an understanding of DNA unwinding and translocation behavior of helicases and motor proteins (Byrd & Raney, 2012; Gilhooly, Gwynn, & Dillingham, 2013; Lohman, Tomko, & Wu, 2008; Pyle, 2008; Singleton, Dillingham, & Wigley, 2007; Spies, Dillingham, & Kowalczykowski, 2005). Though important, these methods measure the average behavior of an ensemble of molecules.…”

Section: Introductionmentioning

confidence: 99%

Direct Fluorescent Imaging of Translocation and Unwinding by Individual DNA Helicases

Pavankumar¹,

Exell²,

Kowalczykowski³

2016

Single-Molecule Enzymology: Fluorescence-Based and High-Throughput Methods

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The unique translocation and DNA unwinding properties of DNA helicases can be concealed by the stochastic behavior of enzyme molecules within the necessarily large populations used in ensemble experiments. With recent technological advances, the direct visualization of helicases acting on individual DNA molecules has contributed significantly to the current understanding of their mechanisms of action and biological functions. The combination of single-molecule techniques that enable both manipulation of individual protein or DNA molecules and visualization of their actions has made it possible to literally see novel and unique biochemical characteristics that were previously masked. Here, we describe the execution and use of single-molecule fluorescence imaging techniques, focusing on methods that include optical trapping in conjunction with epifluorescent imaging, and also surface immobilization in conjunction with total internal reflection fluorescence visualization. Combined with microchannel flow cells and microfluidic control, these methods allow individual fluorescently labeled protein and DNA molecules to be imaged and tracked, affording measurement of DNA unwinding and translocation at single-molecule resolution.

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Superfamily 2 helicases

Cited by 115 publications

References 168 publications

MAPK/ERK1/2 signaling mediates endothelial-like differentiation of immature DCs in the microenvironment of esophageal squamous cell carcinoma

MAPK/ERK1/2 signaling mediates endothelial-like differentiation of immature DCs in the microenvironment of esophageal squamous cell carcinoma

A Parallel Quadruplex DNA Is Bound Tightly but Unfolded Slowly by Pif1 Helicase

Direct Fluorescent Imaging of Translocation and Unwinding by Individual DNA Helicases

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