Superexpression of tuberculosis antigens in plant leaves

Dorokhov, Yuri L.; Sheveleva, Anna; Frolova, Olga; Komarova, Tatjana V.; Zvereva, Anna S.; Иванов, П.А.; Atabekov, J.G.

doi:10.1016/j.tube.2006.10.001

Cited by 46 publications

(28 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our findings indicate that ESAT-6 and CFP-10 should be expressed in a fusion form with a larger "carrier" molecule, in order to accumulate the two T-cell antigens for the production of plant-derived TB vaccines. Consistent with this idea, previous attempts to express in Arabidopsis or tobacco plants were achieved by fusing ESAT-6 either to the enterotoxigenic Escherichia coli heat-labile toxin B-subunit, or to PVX coat protein, or to other proteins (Rigano et al, 2004;2006;Zelada et al, 2006;Dorokhov et al, 2007). These tagged proteins are used, not only as a carrier to protect the antigen, but also as an adjuvant or even as antigens to elicit mucosal immunity.…”

Section: Discussionmentioning

confidence: 99%

Expression of highly immunogenic tuberculosis proteins in plants using a versatilePotato virus X-based expression system

Zeng

Zhang

et al. 2008

The Journal of Horticultural Science and Biotechnology

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Expression of highly immunogenic tuberculosis proteins in plants using a versatilePotato virus X-based expression system

Zeng

Zhang

et al. 2008

The Journal of Horticultural Science and Biotechnology

View full text Add to dashboard Cite

“…Dorokhov et al ( 2007 ) A complete review about plant-made vaccines was published by Awale et al ( 2012 ). Information about the use of plant-made immunogens for developing veterinary vaccines is presented in Chap.…”

Section: Vaccinesmentioning

confidence: 99%

Molecular Farming in Plants

Álvarez

2014

Plant Biotechnology for Health

View full text Add to dashboard Cite

Molecular farming can generally be defi ned as the production of molecules (proteins, fatty acids) for the pharmaceutical and chemical industries in transgenic organisms (plants, animals, etc.).Molecular farming in plants has advantageous aspects as biosecurity, they do not bear pathogens for humans or animals, and they do not produce toxins. Also, plant protein synthetic machinery is able to produce complex glycosylated proteins as they have a glycosylation pattern with slight difference respect of that of mammals, and can perform foldings.When displayed in in vitro conditions, molecular farming have the characteristic advantages of that type of culture, mainly the capacity of working under Good Manufactory practices as is required by the pharmaceutical industry. The critical aspects of plants for molecular farming are the different glycosylation patterns respect to mammals, the duration of the productive process, and the relatively low yields.The approval by the FDA of the fi rst medicine to be used in humans, taliglucerase alfa (ELELYSO®), has give an impulse to this technology but there are some drawbacks to be addressed, particularly related to low yields and regulatory aspects.

show abstract

“…Moreover, they demonstrated in animal models that the plant-made antigens provided high levels of protection against aerosolized plague bacteria. A different group [19] has also achieved expression levels of 0.8 mg/g when expressing the tuberculosis Ag85B antigen. At present, over 50 proteins of different origin and complexity representing all classes of pharmaceutical proteins have been expressed successfully using magnifection [15].…”

Section: Second-generation Vectors: Deconstructed Vectors and Magnifementioning

confidence: 99%