Supercritical fluids extraction and anti-solvent purification of carotenoids from microalgae and associated bioactivity

Extraction and Characterization of Bioactive Compounds with Health Benefits from Marine Resources: Macro and Micro Algae, Cyanobacteria, and Invertebrates

“…Several examples can be found in the literature about integrated processes that may favor the extraction and purifi cation of bioactives; some of them already deal with some green processes to extract bioactive compounds from marine resources (Liau et al 2010 ) whereas others can be used as a base for converting the reported processes to more green, sustainable, and effi cient ones (Siriwardhana et al 2008 ;Athukorala et al 2006 ) . In the fi rst approach (Liau et al 2010 ) , a process was studied considering SFE of lipids and carotenoids from the microalgal species of Nannochloropsis oculata and supercritical antisolvent precipitation of a carotenoidrich solution.…”

“…In the fi rst approach (Liau et al 2010 ) , a process was studied considering SFE of lipids and carotenoids from the microalgal species of Nannochloropsis oculata and supercritical antisolvent precipitation of a carotenoidrich solution. In this approach, both processes were considered independently but it can be easily inferred that the development of a multiple integrated process can allow purifi cation of carotenoids in a cleaner and effi cient way.…”

A review on the extraction of lipid from microalgae for biodiesel production

“…They also reported that Soxhlet extraction using hexane took 5.6 times the time required for SCCO 2 extraction for achieving a comparable lipid yield of 0.058 g lipid/g dried microalgae and using co-solvents like ethanol with CO 2 could increase the lipid extraction yield in SCCO 2 extraction. Liau et al [45] used co-solvent modified SCCO 2 extraction of lipids from the microalgae, N. oculata and reported that at 35 MPa, 50°C and 16.7 of weight percentage of ethanol addition as co-solvent yielded 239.7 mg of triglycerides per gram of extracted oil.…”

Primary Production

“…ethanol, ethyl acetate) or supercritical fluid extraction (SFE) using carbon dioxide can be applied sequentially to separate products according to their polarity. Both extraction methods operate at high pressure and moderate temperature and can thus preserve the nutritional value and techno-functionality of the recovered compounds (Liau et al 2010;Mendes et al 2003;Pieber et al 2012). Furthermore, there are several suitable solvents that meet the requirements and regulations of the food and feed sectors.…”