Supercritical Fluid Extraction of Usnic Acid from Lichen of Cladonia Genus

Бровко, О. С.; Ивахнов, А. Д.; Паламарчук, И. А.; Бойцова, Т. А.

doi:10.1134/s1990793117080024

Cited by 13 publications

(10 citation statements)

References 12 publications

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“…The changes in the content of acids in lichens growing in different environments is of particular interest. Our studies show that the content of UA, a typical secondary metabolite of lichens (Brovko et al 2017), in Usnea sub floridana and Cladonia stellaris varied depending where they grew in the TK zone (Fig. 4).…”

Section: Resultsmentioning

confidence: 73%

“…Phenolic compounds (PCs) is one of the most common classes of secondary metabolites, the formation of which is characteristic of virtually all plant cells. They take part in a wide variety of physiological processes, such as photosynthesis, respiration, formation of cell walls and resistance of plants to the action of heavy metals (Brovko et al 2017). In the literature, there are more than 1000 PCs characteristic of lichens, the so-called "li- chen substances" (Molnar and Farkas 2010), of which the most commonly found is tallow and the well-known usnic acid (UA) (Brovko et al 2017).…”

Section: Resultsmentioning

confidence: 99%

“…The change in the staining intensity of the control and test sample is proportional to the amount of АА in the plant extract. Usnic acid (UA) was extracted using acetone and quantified using a LCMS-2020 liquid quadrupole LCMmass spectrometer (Shimadzu, Japan) according to (Brovko et al 2017). Chromatography conditions were: mobile phase -0.5% aqueous solution of formic acid and acetonitrile in a volume ratio of 30:70; column Restek Ultra C18 100 mm (length) × 3 mm (diameter), fixed phase grain size 3 μm, mobile phase flow rate 0.5 ml min −1 , sample introduction volume 5 μl.…”

Section: Estimation Of Acidsmentioning

confidence: 99%

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Influence of tectonic faults on the conditions and properties of some components of a biogeocenosis in a subarctic area

Belyaev

Bogolytsyn

Бровко

et al. 2019

European Journal of Environmental Sciences

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In geoecology, the nature of the effect of tectonic faults on the environment is not well studied. The influence of natural landscapegeochemical factors on the state and properties of some components of biota of different hierarchical levels is studied at the intersection of two tectonic faults (Velsko-Ust'yanskiy tectonic knot (TK)) in the Arkhangelsk region. Two species of shrubby lichens (Cladonia stellaris Opiz. and Usnea subfloridana Stirt.) and woody plants (spruce -Picea abies L. and Scots pine -Pinus sylvestris L.) were chosen as test systems. The field studies were carried out at nine test sites (in the centre, on the periphery and some distance from TK -the background reference point) in different types of forest. The ash content of samples of the lichen Cladonia growing in the centre of the TK (1.12-1.22%) is double that in the control area (0.56-0.58%), and for the lichen Usnea, it is seven times higher (6.82-6.99% at the centre and 0.97-1.09% in the control area). The ash content of tree bark collected at the centre of TK (1.27-1.29%) is double that at the control site (0.56-0.76%). This indicates a significant accumulation of metals in the vegetation in the TK zone. The accumulation of heavy metals, the low water content of plants, the influence of geomagnetic fields and other factors provoke excessive generation of active oxygen radicals and plants have various physiological, biochemical and morpho-biometric means of combating their adverse effects. The synergism of the cooperative protective action of lichen matrix components on oxidative stress is expressed in terms of changes in biochemical parameters. At the centre of the TK, the lichens contains up to 190 μg g −1 of ascorbic acid, whereas in the control area it does not exceed 130 μg g −1 . The content of usninic acid in the centre is 1.5-2 times higher for the Usnea subfloridana and is 1.5 times higher for Cladonia stellaris compared to the level in the control area.

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Section: Resultsmentioning

confidence: 73%

Section: Resultsmentioning

confidence: 99%

Section: Estimation Of Acidsmentioning

confidence: 99%

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Influence of tectonic faults on the conditions and properties of some components of a biogeocenosis in a subarctic area

Belyaev

Bogolytsyn

Бровко

et al. 2019

European Journal of Environmental Sciences

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“…The methods so far commonly used by various authors for usnic acid extraction have been mainly conventional techniques, with heat reflux (König & Wright 1999; Piska et al 2018), Soxhlet (Einarsdottir et al 2010; Honda et al 2010) or shaking (Yilmaz et al 2004; Roach et al 2006) extractions, while a green technology approach was represented by ultrasound-assisted (Burlando et al 2009; Brovko et al 2017) or supercritical fluid (Brovko et al 2017) extractions. It is interesting to note that these methods are most often used when analytical aspects of usnic acid or its pharmacological activity are of interest, while in the majority of ecologically directed studies, a simple, room-temperature maceration is a common approach (Nybakken et al 2010; Asplund et al 2017).…”

Section: Resultsmentioning

confidence: 99%

“…Even though there are many reports describing the extraction of usnic acid from natural sources by different methods (König & Wright 1999; Yilmaz et al 2004; Roach et al 2006; Burlando et al 2009; Einarsdottir et al 2010; Honda et al 2010; Brovko et al 2017; Piska et al 2018), they do not provide reliable information on the most effective conditions. This discrepancy and the incompleteness of data concerning the extraction parameters for usnic acid have motivated us to explore this issue in more detail.…”

Section: Introductionmentioning

confidence: 99%

Optimization of usnic acid extraction conditions using fractional factorial design

et al. 2020

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Usnic acid is a unique lichen metabolite of industrial importance, widely studied to explore its pharmacological potential and valued especially as an antibacterial agent in cosmetics. Although a vast number of papers describe usnic acid extraction from various lichen species, none has so far provided an unequivocal indication of the best extraction procedure for this compound. Thus, the current study was focused on the direct comparison of three commonly used usnic acid extraction methods (heat reflux, shaking, ultrasound-assisted extractions), which were optimized using fractional factorial design. Heat reflux extraction, shaking extraction and ultrasound-assisted extraction were first optimized in a series of experiments using fractional factorial design, with respect to three parameters: the extraction time, the solvent used and the number of extraction repetitions. HPLC was employed for usnic acid quantitative analysis. The best scores for each extraction method were statistically compared and the optimal conditions were indicated. The optimal set of parameters for usnic acid was established to be a single, 60 min heat reflux extraction with acetone. This extraction scheme provided 4.25 ±0.08 mg g−1 d.w. of usnic acid, while for ultrasound-assisted and shaking extractions the amount was two- or even four times lower (2.33 ±0.17 and 0.97 ±0.08 mg g−1 d.w., respectively). The optimal procedure for usnic acid extraction described here may be suitable for effective acquisition of this compound for scientific research purposes, but also for applications in the pharmaceutical or cosmetic industries.

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Comparative Perspectives on Extraction Methods for Organic Metabolites and Pollutants from Lichens

Forbes

Wat

Strumpher

2020

Lichen‐Derived Products

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Supercritical Fluid Extraction of Usnic Acid from Lichen of Cladonia Genus

Cited by 13 publications

References 12 publications

Influence of tectonic faults on the conditions and properties of some components of a biogeocenosis in a subarctic area

Influence of tectonic faults on the conditions and properties of some components of a biogeocenosis in a subarctic area

Optimization of usnic acid extraction conditions using fractional factorial design

Comparative Perspectives on Extraction Methods for Organic Metabolites and Pollutants from Lichens

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