Supercritical fluid extraction of microbial phospholipid fatty acids from activated sludge

Hanif, M.; Atsuta, Yoichi; Fujie, Koichi; Daimon, Hiroyuki

doi:10.1016/j.chroma.2010.05.027

Cited by 8 publications

(10 citation statements)

References 31 publications

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“…The extraction of analytes from solid samples in environmental applications presents added complications, because the solute‐matrix interactions are very difficult to predict and overcome . In the literature we can find several extraction methods employed for different compounds in solid samples: ultrasonic extraction, USE (acidic pharmaceuticals and phenolic endocrine disrupting chemicals in sewage sludge) , soxhlet extraction (4‐nonylphenols, phthalates and polychlorinated biphenyls in soils and biosolids) , supercritical fluid extraction (microbial phospholipid fatty acids in activated sludge) , accelerated solvent extraction (ASE), (synthetic musks in lake sediment) , pressurised fluid extraction (polycyclic aromatic hydrocarbons in urban street dust) and focussed microwave‐assisted extraction (theobromine and caffeine in cacao) .…”

Section: Introductionmentioning

confidence: 99%

Microwave‐assisted extraction combined with on‐line solid phase extraction followed by ultra‐high‐performance liquid chromatography with tandem mass spectrometric determination of benzotriazole UV stabilizers in marine sediments and sewage sludges

Montesdeoca-Esponda

Sosa‐Ferrera

Santana‐Rodríguez

2013

J of Separation Science

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Benzotriazole ultra-violet stabilisers are compounds widely used in personal care products, which can reach the environment after passing through wastewater treatment plants. In this work, we develop a novel method to evaluate the presence of seven compounds in marine sediments and sewage sludges using microwave-assisted extraction followed by a clean-up step based in on-line solid phase extraction coupled to ultra-high-performance liquid chromatography with MS/MS detection. This method allows for fast and efficient extraction from the solid matrix, subsequent automatic on-line purification and preconcentration, and analysis. For the optimised method, LOD were from 53.3 to 146 ng/kg and LOQ were in the range of 176-486 ng/kg. The method was validated for different environmental solid samples with satisfactory recoveries and relative standard deviations, between 46.1 and 83.9 and 7.8 and 15.5% (sludges) and 50.1 and 87.1% and 8.83 and 16.3% (sediments), respectively. Finally, the studied analytes were quantified in concentrations between 0.18 and 24.0 ng/g in real samples of marine sediments and sewage sludges from Gran Canaria Island (Spain).

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Section: Introductionmentioning

confidence: 99%

Microwave‐assisted extraction combined with on‐line solid phase extraction followed by ultra‐high‐performance liquid chromatography with tandem mass spectrometric determination of benzotriazole UV stabilizers in marine sediments and sewage sludges

Montesdeoca-Esponda

Sosa‐Ferrera

Santana‐Rodríguez

2013

J of Separation Science

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“…The range of pressures, temperatures and modifier concentrations were defined by considering the deterioration of bacterial and archaeal cell with increasing temperature [21,22], and the alteration of cell structure with increasing pressure [19]. Therefore, the effects of pressure (10–30 MPa), temperature (60–100 °C) and modifier concentration (5–15%, v/v) on the total amounts of RQ, PLFA, and PLEL extracted were investigated.…”

Section: Resultsmentioning

confidence: 99%

“…Supercritical carbon dioxide (scCO 2 ) extraction has previously demonstrated its efficiency in the analysis of microbial RQ [21] and PLFA [22] in activated sludge. Characterization of lipid biomarkers profiles using a three-step extraction system, including ‘flash’ SFE, SFE, and enhanced solvent extraction (ESE) has been proposed elsewhere [23].…”

Section: Introductionmentioning

confidence: 99%

Supercritical Fluid Extraction of Bacterial and Archaeal Lipid Biomarkers from Anaerobically Digested Sludge

Hanif

Atsuta

Fujie

et al. 2012

IJMS

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Supercritical fluid extraction (SFE) was used in the analysis of bacterial respiratory quinone (RQ), bacterial phospholipid fatty acid (PLFA), and archaeal phospholipid ether lipid (PLEL) from anaerobically digested sludge. Bacterial RQ were determined using ultra performance liquid chromatography (UPLC). Determination of bacterial PLFA and archaeal PLEL was simultaneously performed using gas chromatography-mass spectrometry (GC-MS). The effects of pressure, temperature, and modifier concentration on the total amounts of RQ, PLFA, and PLEL were investigated by 23 experiments with five settings chosen for each variable. The optimal extraction conditions that were obtained through a multiple-response optimization included a pressure of 23.6 MPa, temperature of 77.6 °C, and 10.6% (v/v) of methanol as the modifier. Thirty nine components of microbial lipid biomarkers were identified in the anaerobically digested sludge. Overall, the SFE method proved to be more effective, rapid, and quantitative for simultaneously extracting bacterial and archaeal lipid biomarkers, compared to conventional organic solvent extraction. This work shows the potential application of SFE as a routine method for the comprehensive analysis of microbial community structures in environmental assessments using the lipid biomarkers profile.

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“…Supercritical CO 2 is usually used to extract non-polar lipids but due to the introduction of co-solvents such as methanol, ethanol and water, it could recover polar lipids [143]. For instance, Hanif et al increased the yield of phospholipid fatty acids (PLFAs) from 0.5 to 7.28 nmol/mg using methanol (10%, v/v) as a co-solvent [145].…”

Section: Supercritical Co 2 Extractionmentioning

confidence: 99%

Biodiesel Production as a Renewable Resource for the Potential Displacement of the Petroleum Diesel

Edeh¹

2020

Biorefinery Concepts, Energy and Products

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In the quest to comply with the Intergovernmental Panel on Climate Change (IPCC) on reducing the global temperature to 1.5-2.0°C as a measure to minimize the climate change caused by the emission of greenhouse gases from the combustion of fossil fuels, and the need for replacement of these fossil fuels, which are also generally believed to be depleting, biodiesel is being studied as a potential replacement for the conventional petroleum diesel. This fuel among other desired properties is renewable, biodegradable, sustainable, and emits less particles. It also contains no amount of sulfur, in addition to possessing most of the good characteristics of petroleum diesel. At the moment, more than 95% of biodiesel produced globally is obtained from vegetable oil feedstocks, which are usually very expensive and thus, without tax waiver and subsidy, makes biodiesel non-competitive with the petroleum diesel. Based on this, non-edible feedstocks are being investigated. Although, their oil yield is low, studies are carried out to ensure efficient extraction. The economics of the process is considered to determine the most economic variables that impact the profitability of biodiesel production. This chapter deals with the biodiesel classification, feedstocks, lipid/oil extraction, biodiesel production methods and the economic aspect of the process.

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Supercritical fluid extraction of microbial phospholipid fatty acids from activated sludge

Cited by 8 publications

References 31 publications

Microwave‐assisted extraction combined with on‐line solid phase extraction followed by ultra‐high‐performance liquid chromatography with tandem mass spectrometric determination of benzotriazole UV stabilizers in marine sediments and sewage sludges

Microwave‐assisted extraction combined with on‐line solid phase extraction followed by ultra‐high‐performance liquid chromatography with tandem mass spectrometric determination of benzotriazole UV stabilizers in marine sediments and sewage sludges

Supercritical Fluid Extraction of Bacterial and Archaeal Lipid Biomarkers from Anaerobically Digested Sludge

Biodiesel Production as a Renewable Resource for the Potential Displacement of the Petroleum Diesel

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