Supercritical fluid extraction of bioactive lipids from the microalga
 
 Nannochloropsis 
 
 sp.

Andrich, Gianpaolo; Nesti, U.; Venturi, Francesca; Zinnai, Angela; Fiorentini, Roberto

doi:10.1002/ejlt.200501130

Cited by 161 publications

(109 citation statements)

References 22 publications

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“…Andrich et al (2005) demonstrated that for ScCO 2 to extract the same amount of lipids from Nannochloropsis sp. as hexane, the extraction should occur at 55°C and 70 MPa.…”

Section: Supercritical Fluid Extractionmentioning

confidence: 99%

A biorefinery for Nannochloropsis: Induction, harvesting, and extraction of EPA-rich oil and high-value protein

Chua

Schenk

2017

Bioresource Technology

117

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Please cite this article as: Chua, E.T., Schenk, P.M., A biorefinery for Nannochloropsis: induction, harvesting, and extraction of EPA-rich oil and high-value protein, Bioresource Technology (2017), doi: http://dx.doi.org/10. 1016/ j.biortech.2017.05.124 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. AbstractMicroalgae have been studied as biofactories for almost four decades. Yet, even until today, many aspects of microalgae farming and processing are still considered exploratory because of the uniqueness of each microalgal species. Thus, it is important to develop the entire process of microalgae farming: from culturing to harvesting, and down to extracting the desired high-value products. Based on its rapid growth and high oil productivities, Nannochloropsis sp. is of particular interest to many industries for the production of highvalue oil containing omega-3 fatty acids, specifically eicosapentaenoic acid (EPA), but also several other products. This review compares the various techniques for induction, harvesting, and extraction of EPA-rich oil and high-value protein explored by academia and industry to develop a multi-product Nannochloropsis biorefinery. Knowledge gaps and opportunities are discussed for culturing and inducing fatty acid biosynthesis, biomass harvesting, and extracting EPA-rich oil and high-value protein from the biomass of Nannochloropsis sp.

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“…Andrich et al (2005) demonstrated that for ScCO 2 to extract the same amount of lipids from Nannochloropsis sp. as hexane, the extraction should occur at 55°C and 70 MPa.…”

Section: Supercritical Fluid Extractionmentioning

confidence: 99%

A biorefinery for Nannochloropsis: Induction, harvesting, and extraction of EPA-rich oil and high-value protein

Chua

Schenk

2017

Bioresource Technology

117

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“…could be integrated, adopting some simplifying hypothesis [1,5] and utilising the equation related to mass balance of oil between the two involved phases: …”

Section: Kinetic Evaluation Of Oil Extractionmentioning

confidence: 99%

“…Among the alternative solvents proposed, supercritical CO 2 (Sc-CO 2 ) has been investigated for three microalgae (Nannochloropsis sp., Schizochytrium sp. and Spirulina (Arthrospira) platensis) [1][2][3] and for three oilseeds (sunflower, rape and soybean) [4][5][6].…”

Section: Introductionmentioning

confidence: 99%

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A Simplified Method to Estimate the Time Evolution of Oil Extraction from Different Substrates by Supercritical CO2

Zinnai¹,

Venturi²,

Sanmartin³

et al. 2012

AJAC

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To allow an easy individuation of the more suitable working conditions (temperature, pressure, flow rate, etc.) to be adopted to carry out the extraction of food grade oils from different substrates by supercritical CO 2 (Sc-CO 2 ), a simplified kinetic approach has been introduced. This kinetic model was utilised to describe supercritical fluid extraction (SFE) of oil by Sc-CO 2 not only from seeds (sunflower, soybean and rape) but also from microalgae (Nannochloropsis sp., Schizochytrium sp. and Spirulina (Arthrospira) platensis) characterised by a lipid fraction with a high proportion of polyunsatured fatty acids (C20:5ω-3; C22:6ω-3; C18:3ω-6). Thanks to the high affinity occurring between oil and Sc-CO 2 it was possible to introduce a simplified kinetic model able to describe the time evolution of oil extraction from substrates which deeply differ for biochemical and biophysical characteristics. Moreover the synergistic utilisation of the kinetic model introduced and of the Chrastil's equation, allowed to predict the time evolution of oil extraction as a function of the: substrate used; amount of its fat content; mass of substrate charged inside the extractor; possible pretreatments carried out on the used substrate; flow rate of Sc-CO 2 ; working conditions adopted (temperature, pressure and then Sc-CO 2 density).

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“…When carbon dioxide is heated beyond its critical temperature and pressure, it reaches a supercritical state and although it behaves like a gas, it also exhibits some characteristics of a liquid solvent (Andrich, Nesti, Venturi, Zinnai, & Fiorentini, 2005). These technologies have not been studied exhaustively for the extraction of lipids from microalgae.…”

Section: Bligh and W J Dyer Of The Fisheries Research Board Of Canamentioning

confidence: 99%

Extraction and Characterization of Lipids From Microalgae Grown on Municipal Wastewater

Hutton¹

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Supercritical fluid extraction of bioactive lipids from the microalga Nannochloropsis sp.

Cited by 161 publications

References 22 publications

A biorefinery for Nannochloropsis: Induction, harvesting, and extraction of EPA-rich oil and high-value protein

A biorefinery for Nannochloropsis: Induction, harvesting, and extraction of EPA-rich oil and high-value protein

A Simplified Method to Estimate the Time Evolution of Oil Extraction from Different Substrates by Supercritical CO<sub>2</sub>

Extraction and Characterization of Lipids From Microalgae Grown on Municipal Wastewater

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Supercritical fluid extraction of bioactive lipids from the microalga Nannochloropsis sp.

Cited by 161 publications

References 22 publications

A biorefinery for Nannochloropsis: Induction, harvesting, and extraction of EPA-rich oil and high-value protein

A biorefinery for Nannochloropsis: Induction, harvesting, and extraction of EPA-rich oil and high-value protein

A Simplified Method to Estimate the Time Evolution of Oil Extraction from Different Substrates by Supercritical CO&lt;sub&gt;2&lt;/sub&gt;

Extraction and Characterization of Lipids From Microalgae Grown on Municipal Wastewater

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A Simplified Method to Estimate the Time Evolution of Oil Extraction from Different Substrates by Supercritical CO<sub>2</sub>