Supercritical Fluid Extraction Enhances Discovery of Secondary Metabolites from Myxobacteria

Bader, Chantal D.; Neuber, Markus; Panter, Fabian; Krug, Daniel; Müller, Rolf

doi:10.1021/acs.analchem.0c02995

Cited by 19 publications

(10 citation statements)

References 52 publications

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“…from crude extracts if possible, without the need for small-scale fractionation and enrichment). This objective can be supported by innovative extraction methods prior to bioactivity-guided isolation of novel compounds 188 .…”

Section: Natural-product-based Hit Compoundsmentioning

confidence: 99%

Towards the sustainable discovery and development of new antibiotics

et al. 2021

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An ever-increasing demand for novel antimicrobials to treat life-threatening infections caused by the global spread of multidrug-resistant bacterial pathogens stands in stark contrast to the current level of investment in their development, particularly in the fields of natural-product-derived and synthetic small molecules. New agents displaying innovative chemistry and modes of action are desperately needed worldwide to tackle the public health menace posed by antimicrobial resistance. Here, our consortium presents a strategic blueprint to substantially improve our ability to discover and develop new antibiotics. We propose both short-term and long-term solutions to overcome the most urgent limitations in the various sectors of research and funding, aiming to bridge the gap between academic, industrial and political stakeholders, and to unite interdisciplinary expertise in order to efficiently fuel the translational pipeline for the benefit of future generations.

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Section: Natural-product-based Hit Compoundsmentioning

confidence: 99%

Towards the sustainable discovery and development of new antibiotics

et al. 2021

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“…Several reasons can explain this finding; in principle, the extraction of natural metabolites often requires severe time-cost-consuming procedures than crude oil extraction; moreover, the yield of secondary metabolites is often lower when compared to the essential oil recovery. In this respect, many efforts are ongoing to improve the whole extraction procedures by exploiting more efficient green methodologies such as microwave and/or ultrasound assisted extraction [167] and supercritical fluid extraction [168]. In addition, secondary metabolites are volatile and need to be physically or chemically entrapped in protective structures to preserve their bioactivity, as successfully demonstrated by Moeini et al [12].…”

Section: Plasticizing Effectmentioning

confidence: 99%

Edible Polymers and Secondary Bioactive Compounds for Food Packaging Applications: Antimicrobial, Mechanical, and Gas Barrier Properties

et al. 2022

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Edible polymers such as polysaccharides, proteins, and lipids are biodegradable and biocompatible materials applied as a thin layer to the surface of food or inside the package. They enhance food quality by prolonging its shelf-life and avoiding the deterioration phenomena caused by oxidation, humidity, and microbial activity. In order to improve the biopolymer performance, antimicrobial agents and plasticizers are also included in the formulation of the main compounds utilized for edible coating packages. Secondary natural compounds (SC) are molecules not essential for growth produced by some plants, fungi, and microorganisms. SC derived from plants and fungi have attracted much attention in the food packaging industry because of their natural antimicrobial and antioxidant activities and their effect on the biofilm’s mechanical properties. The antimicrobial and antioxidant activities inhibit pathogenic microorganism growth and protect food from oxidation. Furthermore, based on the biopolymer and SC used in the formulation, their specific mass ratio, the peculiar physical interaction occurring between their functional groups, and the experimental procedure adopted for edible coating preparation, the final properties as mechanical resistance and gas barrier properties can be opportunely modulated. This review summarizes the investigations on the antimicrobial, mechanical, and barrier properties of the secondary natural compounds employed in edible biopolymer-based systems used for food packaging materials.

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“…An MIC value of 0.0157 µM was obtained for micrococcin P1. Using the method of Okanya et al [41] and its modification as outlined by Jansen et al [40] and Bader et al [42], the IC50 of micrococcin P1 against S. aureus was shown to be 0.357 µM (0.159 mg/mL) (Figure S8). This value is within the range of IC50 of micrococcin P1 against an assorted collection of S. aureus strains and confirms the activity of micrococcin P1 against S. aureus to be strain dependent [39].…”

Section: Biological Activity Of Micrococcin P1 Against Staphylococcus...mentioning

confidence: 99%

Micrococcin P1 and P2 from Epibiotic Bacteria Associated with Isolates of Moorea producens from Kenya

2022

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Epibiotic bacteria associated with the filamentous marine cyanobacterium Moorea producens were explored as a novel source of antibiotics and to establish whether they can produce cyclodepsipeptides on their own. Here, we report the isolation of micrococcin P1 (1) (C48H49N13O9S6; obs. m/z 1144.21930/572.60381) and micrococcin P2 (2) (C48H47N13O9S6; obs. m/z 1142.20446/571.60370) from a strain of Bacillus marisflavi isolated from M. producens’ filaments. Interestingly, most bacteria isolated from M. producens’ filaments were found to be human pathogens. Stalked diatoms on the filaments suggested a possible terrestrial origin of some epibionts. CuSO4.5H2O assisted differential genomic DNA isolation and phylogenetic analysis showed that a Kenyan strain of M. producens differed from L. majuscula strain CCAP 1446/4 and L. majuscula clones. Organic extracts of the epibiotic bacteria Pseudoalteromonas carrageenovora and Ochrobactrum anthropi did not produce cyclodepsipeptides. Further characterization of 24 Firmicutes strains from M. producens identified extracts of B. marisflavi as most active. Our results showed that the genetic basis for synthesizing micrococcin P1 (1), discovered in Bacillus cereus ATCC 14579, is species/strain-dependent and this reinforces the need for molecular identification of M. producens species worldwide and their epibionts. These findings indicate that M. producens-associated bacteria are an overlooked source of antimicrobial compounds.

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Supercritical Fluid Extraction Enhances Discovery of Secondary Metabolites from Myxobacteria

Cited by 19 publications

References 52 publications

Towards the sustainable discovery and development of new antibiotics

Towards the sustainable discovery and development of new antibiotics

Edible Polymers and Secondary Bioactive Compounds for Food Packaging Applications: Antimicrobial, Mechanical, and Gas Barrier Properties

Micrococcin P1 and P2 from Epibiotic Bacteria Associated with Isolates of Moorea producens from Kenya

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