Supercritical fluid extraction as a method for fat content determination and preparative technique for fatty acid analysis in mesh feed for pigs

Ivanov, D.; Čolović, Radmilo; Bera, Oskar; Lević, J.; Sredanović, S.

doi:10.1007/s00217-011-1521-x

Cited by 15 publications

(11 citation statements)

References 22 publications

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“…Snyder and co‐workers also confirmed agreement between SFE‐based methods and solvent extraction‐based methods for meat samples, by obtaining almost identical fatty acid composition, independently of the source of the constituent fatty acid 40. Other sources reported that SFE extracts can be used for fatty acid analysis, unlike extracts obtained by Soxhlet extraction 25.…”

Section: Resultsmentioning

confidence: 75%

“…It has been noticed that amount of added ethanol is directly proportional to solubility increase of phospholipids‐rich fraction 12. The same increasing trend in extracts yield was proven for SFE of fat in corn‐based pig feed 25. Salgin in his work thoroughly examined the effect of CO 2 + ethanol mixture on the extraction of jojoba seed oil in green and high‐tech separation process, emphasising the effect on yield, solubility and the initial time of extraction 26.…”

Section: Resultsmentioning

confidence: 84%

“…Comparison of the results for fatty acid analysis of extracts obtained by SFE and Soxhlet extraction, shows that content of α‐linolenic acid (C18:3 n‐ 3) is significantly higher ( p = 0.007) for approximately 4% in SFE extracts, than in Soxhlet extracts, while content of palmitic acid (C16:0) and oleic acid (C18:1) are higher in Soxhlet extracts (Table 4). It can be concluded that Soxhlet extraction caused degradation of double bonds of polyunsaturated fatty acid C18:3 n‐ 3, probably due to the oxidation 25. Since the main nutritional benefit of linseed comes from high content of C18:3 n‐ 3 fatty acid, the results obtained by analyzing Soxhlet extracts gave incorrect data about one of its most important nutritive characteristic.…”

Section: Resultsmentioning

confidence: 99%

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Optimization of supercritical fluid extraction of linseed oil using RSM

Ivanov

Čolović

Lević

et al. 2012

Euro J Lipid Sci & Tech

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The objective of the present study was to develop a supercritical fluid extraction (SFE) method, suitable for extraction of total oil content from linseed, and to be used as a preparative technique for fatty acid determination. Optimum conditions (volume of added ethanol as a co-solvent, dynamic extraction time (DET), and pressure) were predicted in order to obtain the maximum yield of the extract. Response surface methodology (RSM) and central composite rotatable design (CCRD) were used for modeling the process. Variable values ranged as follows: co-solvent 0-1 mL, DET 36-60 min, and pressure 45.57-62.05 MPa (6000-9000 psi). Effects of co-solvent volume and extraction pressure were well described by simplified polynomial equation (R 2 ¼ 0.85), since DET had no significant influence ( p>0.05) on the extract yield. The maximum yield of oil, calculated from experimental results, was obtained with 1 mL of co-solvent, and pressure of 62.05 MPa. Optimized conditions were used for extraction of oil from four samples of linseed, ground to pass through 1, 2, 3, and 4 mm-sieve, to determine adequate granulation for SFE. Finally, results for yield and fatty acid composition of the extract obtained using SFE were compared with the results of Soxhlet extraction.Practical applications: The obtained extracts can be used for fatty acid analysis, since they have not been damaged and their fatty acid compositions have not been degraded by reagents or aggressive extraction conditions. It is shown that the selection of appropriate milling equipment for grinding of samples is necessary to achieve adequate granulation and avoid fractionation of sample.

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Section: Resultsmentioning

confidence: 75%

Section: Resultsmentioning

confidence: 84%

Section: Resultsmentioning

confidence: 99%

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Optimization of supercritical fluid extraction of linseed oil using RSM

Ivanov

Čolović

Lević

et al. 2012

Euro J Lipid Sci & Tech

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“…Supercritical fluid extraction with CO 2 was used for preparation of fat extracts, as recommended for fatty acid analysis (Ivanov et al, 2011). Extractions were performed on a LECO TFA2000 fat analyzer with methods developed in the laboratory.…”

Section: Fat Extraction For Fatty Acid Analysismentioning

confidence: 99%

Meat fatty acid profile of pigs fed linseed enriched diet

et al. 2012

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The aim of this study was to evaluate the influence of diet supplemented with linseed rich additive on fatty acid profile and omega-fatty acids content in pig meat. Twelve pigs were divided in a control and experimental group and grown to 110 kg of live weight. The experimental group was fed a standard diet enriched with 2.5% of commercial additive Vitalan ®. After the end of feeding period, the meat samples from both groups were analyzed for fatty acids content in raw and roasted meat. The ratio between omega-6 and omega-3 acids was established. Samples from experimental group fed with linseed enriched diet showed higher ω-3 acids content in meat (6.24%-7.23%), compared to the control group (0.71%-1.64%), thus making it better for a human consumption from a health perspective. Linseed enriched diet positively influenced fatty acid composition of pig M. Longissimus dorsi muscle by decreasing SFA content, as well as increasing PUFA and UFA content. Heat treatment did not significantly decreased content of stearic and linoleic fatty acids in any sample. It was concluded that the diet enriched with extruded linseed had beneficial effect on the majority of monitored parameters in the study.

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“…A utilização de fluidos supercríticos em processos de extração abrange várias vertentes dentro da química analítica como por exemplo, extração de óleos essenciais de plantas, aplicação em produtos alimentícios e também, na área ambiental, para análises de inúmeras classes de contaminantes. 8,9,10 Dentro desse contexto, surgiu o interesse em aplicar a SFE como meio de remover poluentes de solo, visto que o grupo de cromatografia do Instituto de Química de São Carlos (CROMA) há tempos converge esforços para o desenvolvimento tanto da instrumentação quanto para a aplicação de novas metodologias utilizando fluidos no estado supercrítico [11][12][13][14][15] e também devido a aplicabilidade e eficiência já reportada referente a essa técnica. 16,17,18…”

Section: Introductionunclassified

Utilização de técnicas compatíveis com o conceito de química verde na determinação de micropoluentes orgânicos em matrizes ambientais

Franco¹

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Ao Professor Fernando Mauro Lanças, por ter me recebido em seu grupo de pesquisa e confiado no meu trabalho, me orientando ao longo desses anos e, sem dúvida, contribuindo para meu crescimento profissional. A minha mãe, Lenice, e minha irmã, Maressa, que sempre estiveram ao meu lado, apoiando minhas decisões, me fazendo seguir em frente e acreditando sempre nos meus ideais. Ao Rodrigo, que sonhou junto a mim todos esses anos, acreditando no meu potencial e me ajudando a superar as dificuldades. Com certeza, perfeito namorado e melhor companheiro de trabalho. Aos amigos do CROMA, que me ofereceram não só conhecimento cientifico, mais também amizade e companheirismo, dos quais eu jamais me esquecerei. Ao professor Álvaro que, sempre com entusiasmo, colaborou com o desenvolvimento do trabalho, discutindo dados e aperfeiçoando ideias. Ao Dalton, por estar sempre disposto a colaborar com a pesquisa, desenvolvendo junto comigo parte desse trabalho. À Odete, que traz consigo a alma do laboratório, sempre com uma palavra amiga e a mão estendida para nos ajudar. Ao Alcimar, Elaine e Guilherme que se fizeram sempre presentes, me auxiliando e contribuindo para o desenvolvimento do trabalho. À professora Eny, pela amizade e carinho dedicados a mim e ao Rodrigo. Aos funcionários das oficinas mecânica e elétrica pelo suporte técnico prestado, sempre dispostos a solucionarem nossos problemas. Aos funcionários da Associação Internacional de Cromatografia, NST e Protheus pela pronta disposição a colaborar.

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Supercritical fluid extraction as a method for fat content determination and preparative technique for fatty acid analysis in mesh feed for pigs

Cited by 15 publications

References 22 publications

Optimization of supercritical fluid extraction of linseed oil using RSM

Optimization of supercritical fluid extraction of linseed oil using RSM

Meat fatty acid profile of pigs fed linseed enriched diet

Utilização de técnicas compatíveis com o conceito de química verde na determinação de micropoluentes orgânicos em matrizes ambientais

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