Supercritical fluid extraction and high-performance liquid chromatography–fluorescence detection method for polycyclic aromatic hydrocarbons investigation in vegetable oil

Yusty, María Asunción Lage; Daviña, J L Cortizo

doi:10.1016/j.foodcont.2003.11.008

Cited by 79 publications

(19 citation statements)

References 29 publications

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“…Vegetable oils may be contaminated with PAHs as a result of processing, especially drying of oil plants and due to use of contaminated extraction solvent. To a lesser extent a contamination of these oils may be associated with environmental pollution (Guillen et al 2004;Lage Yusty and Cortizo Daviña, 2005;Moret et al 2005;WHO 2010;Bojanowska and Czerwiński 2010); however, in a case of high air pollution with PAHs and due to atmospheric precipitation, a superficial contamination of plants during growing season may occur. This contamination may be then transferred to the final product (Rodríguez-Acuna et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Method Validation and Determination of Polycyclic Aromatic Hydrocarbons in Vegetable Oils by HPLC-FLD

2016

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The aim of this work was to determine the level of contamination of different groups of vegetable oils available on the Polish market with polycyclic aromatic hydrocarbons, i.e. benzo(a)pyrene and sum of benzo(a)pyrene, benz(a)anthracene, benzo(b)fluoranthene and chrysene, the content of which in foodstuffs is limited by Commission Regulation (EU) 2015/1125 of 10 July 2015. The research materials were refined rapeseed oils, sunflower oils, olive pomace oil, rapeseed oils with olive oil and unrefined soybean and coconut oils. The research methods included process of saponification of the vegetable oils, extraction of the polycyclic aromatic hydrocarbons fraction, clean-up by use a column packed with aluminum oxide and elution by petroleum ether, and then quantitative and qualitative determination of by high performance liquid chromatography with fluorescence detection method. Values of limit of detection and limit of quantification obtained during validation of the method were 0.18 and 0.25 μg/kg, respectively, and were significantly lower than the respective maximum values given in Commission Regulation (EU) 836/2011. The highest polyaromatic hydrocarbons content was found in unrefined coconut and soybean oils. The benzo(a)pyrene content and sum of benzo(a)pyrene, benz(a)anthracene, benzo(b)fluoranthene and chrysene in all the tested sample did not exceed the maximum levels given in Commission Regulation (EU) 2015/1125.

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Section: Introductionmentioning

confidence: 99%

Method Validation and Determination of Polycyclic Aromatic Hydrocarbons in Vegetable Oils by HPLC-FLD

2016

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“…It included naphthalene (Nap), acenaphthylene (Acy), acenaphthene (Ace), fluorene (Fl), phenanthrene (Phe), anthracene (Ant), fluoranthene (Flu), pyrene (Pyr), chrysene (Chr), benz [ 12 , and IPd 12 were purchased from C/D/N Isotopes (Pointe-Claire, QC, Canada). The purities of the PAH standards and the internal standards were greater than 97 %.…”

Section: Methodsmentioning

confidence: 99%

“…Peanut and olive oils showed higher PAH levels than soybean oils because they were not refined, a process with a recognized eliminating effect on PAH content [6,12,39]. Meanwhile, extremely high PAH values of peanut oils were observed.…”

Section: Pahs In Commercial Edible Vegetable Oilmentioning

confidence: 99%

“…Commonly used preparation techniques usually include an extraction procedure (e.g., liquid-liquid extraction [5,10], pressurized liquid extraction [11], supercritical fluid extraction [12], and solid-phase microextraction [13]) followed by one or more purification steps (e.g., solid-phase extraction [1,7] and gel permeation chromatography [14,15]). For the determination of multiple PAHs in edible oils, both high-performance liquid chromatography (HPLC) coupled with fluorescence detection (FLD) [1,10,12] and gas chromatography (GC)-mass spectrometry (MS) [5,16,17] are widely used. The challenges, advantages, and disadvantages of HPLC-FLD and GC-MS methods applied in PAH analysis have been thoroughly discussed in some research articles [7,15,[18][19][20].…”

Section: Introductionmentioning

confidence: 99%

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One-step solvent extraction followed by liquid chromatography–atmospheric pressure photoionization tandem mass spectrometry for the determination of polycyclic aromatic hydrocarbons in edible oils

Shi

Liu

et al. 2015

Anal Bioanal Chem

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A method for rapid analysis of 16 polycyclic aromatic hydrocarbons (PAHs) in edible oils has been developed on the basis of a simplified solvent extraction and liquid chromatography-atmospheric pressure photoionization tandem mass spectrometry performed in multiple reaction monitoring mode. The briefness of the experimental procedure, the use of milliliters of acetonitrile for extraction without any further cleanup process, the short analysis time, and the excellent sensitivity and selectivity demonstrated the advantages of this practical and environmentally friendly method. All the analytes exhibited satisfactory recoveries at three spiking levels (the recoveries ranged from 77.8 to 106.4%), and the relative standard deviations were lower than 10%. The limits of quantitation of this method for the 16 PAHs were in the range of 0.02-0.43 μg/kg. The validated method was successfully applied for the determination of PAHs in coconut oil reference material (BCR-458) and real edible oil samples. The results suggested that a large-scale investigation of the concentration of PAHs in vegetable oils in China is required.

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“…Entre os métodos utilizados para análise de HPAs em alimentos, a Cromatografia Líquida de Alta Eficiência tem sido amplamente utilizada em diferente matrizes [6,7,10,13,18,19].…”

Section: Determinação De Hpas Em Guaraná Em Pó Camargo Et Alunclassified

Determinação de hidrocarbonetos policíclicos aromáticos (HPAS) em guaraná em pó (Paullinia cupana)

Camargo¹,

Tfouni²,

Vitorino³

et al. 2006

Ciênc. Tecnol. Aliment.

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Supercritical fluid extraction and high-performance liquid chromatography–fluorescence detection method for polycyclic aromatic hydrocarbons investigation in vegetable oil

Cited by 79 publications

References 29 publications

Method Validation and Determination of Polycyclic Aromatic Hydrocarbons in Vegetable Oils by HPLC-FLD

Method Validation and Determination of Polycyclic Aromatic Hydrocarbons in Vegetable Oils by HPLC-FLD

One-step solvent extraction followed by liquid chromatography–atmospheric pressure photoionization tandem mass spectrometry for the determination of polycyclic aromatic hydrocarbons in edible oils

Determinação de hidrocarbonetos policíclicos aromáticos (HPAS) em guaraná em pó (Paullinia cupana)

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