Supercritical fluid extraction and high performance liquid chromatographic determination of benzopyrans and phloroglucinol derivative in Hypericum polyanthemum

Supercritical extraction of phloroglucinol and benzophenone derivatives from Hypericum carinatum: Quantification and mathematical modeling

“…It is well established that benzopyran, benzophenone and phloroglucinol derivatives present in some Hypericum species are soluble in acetone, while undesirable waxes and other compounds remain precipitated [22]. As it can be seen in Table 1, at 401C and 90 bar the yield of the acetonesoluble fraction was very high, representing ca.…”

“…Powdered plant material (100 g DW) was extracted at constant temperature (40, 50 or 601C) while the pressure was successively increased from 90 to 120, 120 to 150 and 150 to 200 bar. The extractions were performed following the experimental procedure defined by Cargnin et al [22], i.e., 100 min at 90 bar, 30 min at 120 bar, 30 min at 150 bar and 30 min at 200 bar, using the same plant material. However, instead of only one fraction for each pressure, in this study samples were collected every 10 min in order to evaluate yield and composition of the extracts versus extraction time.…”

“…The n-hexane and supercritical CO 2 extracts were treated with acetone and filtered off to remove waxes and insoluble impurities [22]. Then, the enriched fractions were evaporated to dryness, dissolved in HPLC-grade methanol, filtered (0.22 mm pore size, Merck) and analyzed by HPLC.…”

“…In all the experiments, at 90 bar, the end time was defined considering the plant exhaustion (100 min). For other pressures (120, 150 and 200 bar), the extraction time was defined as 30 min, following the same experimental procedure used by Cargnin et al [22]. The amounts of uliginosin B, cariphenone A and cariphenone B in the acetone soluble fraction of the n-hexane extract were respectively: 167.9473.76, 58.6071.72, and 26.4070.65 mg per 100 g of plant.…”

“…Nevertheless, until now it was employed only for H. perforatum [8,21] and H. polyanthemum [22]. Thus, this work investigated the effect of extraction conditions [23] such as temperature, pressure and time regarding the recovery of extracts and concentration of uliginosin B, cariphenone A and cariphenone B.…”