Supercritical fluid chromatography/tandem mass spectrometric method for analysis of pharmaceutical compounds in metabolic stability samples

Hsieh, Yunsheng; Favreau, Leonard; Schwerdt, John; Cheng, Kui

doi:10.1016/j.jpba.2005.06.032

Cited by 34 publications

(14 citation statements)

References 21 publications

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“…Fast solvent gradients with short 5 cm chiral columns are currently being evaluated by SFC user groups to increase sample throughput in early discovery stage. Packed column SFC has been demonstrated to offer these advantages for analysis of a wide variety of pharmaceutical compounds [8][9][10][11]. * Corresponding author.…”

Section: Introductionmentioning

confidence: 99%

A feasibility study on direct assay of an aqueous formulation by chiral supercritical fluid chromatography (SFC)

Mukherjee¹,

Cook²

2006

Journal of Pharmaceutical and Biomedical Analysis

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Section: Introductionmentioning

confidence: 99%

A feasibility study on direct assay of an aqueous formulation by chiral supercritical fluid chromatography (SFC)

Mukherjee¹,

Cook²

2006

Journal of Pharmaceutical and Biomedical Analysis

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“…The recent developments in analytical supercritical fluid chromatography (SFC)-MS as a promising and robust approach for small molecule analysis or purification may provide solutions for some of these issues [32][33][34][35]. Still, further improvements are needed, especially for the analysis of enantiomeric purity and molecular identity.…”

Section: Open-access Analysismentioning

confidence: 99%

MS Applications in Support of Medicinal Chemistry Sciences

Honing¹,

Ingelse²,

Pramanik³

2013

Mass Spectrometry for Drug Discovery and Drug Development

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In the drug discovery process, medicinal chemistry plays a crucial role in the optimal design and synthesis (production) of new chemical entities (NCEs) or new biological entities (NBEs); this is true for a single entity as well as a complete compound library. A key requirement in this field of science is the optimization of both the chemical and the biological properties of these potential drug-like compounds. Important criteria are the design of efficient synthesis routes, the balancing of the physical chemical properties with optimal in vitro and in vivo drug metabolism and pharmacokinetic (DMPK) processes, and a profound understanding of the pharmacodynamics in the appropriate pharmacological models. In the lead optimization stage, an important step is scaling up the synthesis route as needed for the execution of toxicology studies and the screening of suitable drug formulations. In summary, medicinal chemistry support includes participating in a large variety of activities and decision making processes that play a key role in the search for either a "first in class" new drug or a "best in class" new drug [1].These "drug discovery" processes are continuously under pressure, as the research and development (R&D) costs continue to increase and more data are requested before new drug candidates are taken into (pre-)clinical development. Hence, the choice of "targeted" high-throughput screening (HTS) libraries, the process of hit appraisal, and the understanding of target-ligand interactions can be considered the starting points for a new drug discovery program [2]. Next, the main focus is on the discovery of a lead compound; typically, this is a compound showing promising data regarding target affinity, reasonable efficacy in cellular models, good physical chemical parameters (i.e., a "drug-like" compound), and acceptable oral bioavailability in Mass Spectrometry for Drug Discovery and Drug Development, First Edition. Edited by Walter A. Korfmacher.

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“…For HPLC-MS, higher mobile-phase flow rates normally result in appreciable negative impact on the ionization efficiency of the analytes. However, the tolerable input of mobile-phase flow rate into the APCI source was enhanced in the presence of supercritical fluid due to its high volatility which efficiently assists the nebulization process [30]. APCI normally generates both [M + H] + and [M -H] -ions to make mass spectral interpretation trivial.…”

Section: Integration Of Sfc-msmentioning

confidence: 99%

Supercritical fluid chromatography‐mass spectrometry for chemical analysis

Li¹,

Hsieh²

2008

J of Separation Science

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Chromatography with a supercritical fluid as the mobile phase was suggested more than four decades ago (Klesper, E., Corwin, A. H., Turner, D. A., J. Org. Chem. 1962, 27, 700-701). Supercritical fluid chromatography (SFC) is basically a hybrid of GC and LC that eases the resolution of a mixture of compounds not conveniently resolved by either GC or LC. The mobile phases for SFC have low viscosities and high diffusion coefficients compared to those for HPLC and allow for high efficiency separations. SFC uses supercritical fluid as the mobile phase, polar organic solvents as the modifiers in conjunction with acidic/basic compounds as additives to run the chromatographic process like in HPLC. In many applications, SFC-based methods are advantageous over HPLC-based methods as a separation tool in terms of efficiency and economical impact perspectives. Today, the availability of commercial hardware and API interfaces with a mass spectrometer makes SFC even more widely applicable for chemical analysis in many research fields. This review summarizes a variety of novel SFC-MS methods for chemical analysis that have been reported in the peer-reviewed publications.

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Supercritical fluid chromatography/tandem mass spectrometric method for analysis of pharmaceutical compounds in metabolic stability samples

Cited by 34 publications

References 21 publications

A feasibility study on direct assay of an aqueous formulation by chiral supercritical fluid chromatography (SFC)

A feasibility study on direct assay of an aqueous formulation by chiral supercritical fluid chromatography (SFC)

MS Applications in Support of Medicinal Chemistry Sciences

Supercritical fluid chromatography‐mass spectrometry for chemical analysis

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