Supercritical Fluid Chromatography–Mass Spectrometry

Thite, Mohini A.

doi:10.1201/b15594-12

Cited by 2 publications

(3 citation statements)

References 42 publications

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“…SFC has several advantages compared to the conventional liquid and gas chromatography [29]. Supercritical carbon-dioxide has lower viscosity and higher diffusion rate (thus faster mass transfer) than the liquids.…”

Section: Introductionmentioning

confidence: 99%

Identification of Triterpenes and β-sitosterol in the Bark of Plane Tree Extracts

Plánder

Simon

Béni

et al. 2019

Period. Polytech. Chem. Eng.

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Plane tree is planted as ornamental tree in urban areas. This tree naturally sheds its bark during the spring; however, the shed bark is commonly regarded as a waste material without any significant application.On the other hand, the bark of plane tree may be an important source of industrially relevant compounds, most notably betulinic acid. In our study a Supercritical Fluid Ultra Performance Convergence Chromatography (UPC2) system coupled with Evaporative Light Scattering Detector (ELSD), along with conventional HPLC, GC-MS and NMR were successfully utilized to analyze triterpenes in the extracts from the bark of plane tree. We show that not only betulinic acid, but other important triterpenes: betulin, betulinic aldehyde and β-sitosterol are also present in the extract of the plane tree bark. Among these the main compound is betulinic acid, with up to an order of magnitude larger concentration than the other constituents. The applied extraction method has a significant role on the concentration of the different compounds in the extracts. Most notably, neat scCO2 is not suitable to extract the polar betulinic acid, however betulin and betulinic aldehyde can be extracted selectively.

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Section: Introductionmentioning

confidence: 99%

Identification of Triterpenes and β-sitosterol in the Bark of Plane Tree Extracts

Plánder

Simon

Béni

et al. 2019

Period. Polytech. Chem. Eng.

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“…The wide chemical diversity of natural products has always challenged analysts. Therefore, the availability of such highly efficient analytical technologies is of tremendous interest [4]. Initially, the application field of SFC in natural product analysis was relatively modest, focusing mainly on nonpolar compounds [5,6].…”

Section: Introductionmentioning

confidence: 99%

“…▶ Fig. 1, the range of applications is broadly diversified and extends from the separation of nonpolar lipids to the analysis of highly polar triterpene saponins with several sugar residues [3,4,7,8].…”

Section: Introductionmentioning

confidence: 99%

Supercritical Fluid Chromatography in Natural Product Analysis – An Update

2017

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The wide chemical diversity of natural products has challenged analysts all over the world and has been a driving force for the development of innovative technologies since decades. In the last years, supercritical fluid chromatography (SFC) has finally emerged from the shadow of liquid chromatography (LC) and gas chromatography (GC) and has become a powerful tool in modern natural product analysis. Whereas in the past the technique had mainly been restricted to a small group of nonpolar compounds, it has largely expanded its suitability in the last years and has demonstrated possibilities without boundaries. This mini-review, focused on the latest applications, provides a brief update on the current status of SFC in natural product analysis with the aim to demonstrate its applicability for both polar and nonpolar plant constituents. The approaches cover the whole range of polarity, including carotenoids, flavonoids, water-unstable ginkgolides, and even highly polar triterpene saponins with several sugar residues.

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Supercritical Fluid Chromatography–Mass Spectrometry

Abstract: When referring to this work, full bibliographic details including the author, title, awarding institution and date of the thesis must be given e.g. AUTHOR (year of submission) "Full thesis title",

Cited by 2 publications

References 42 publications

Identification of Triterpenes and β-sitosterol in the Bark of Plane Tree Extracts

Identification of Triterpenes and β-sitosterol in the Bark of Plane Tree Extracts

Supercritical Fluid Chromatography in Natural Product Analysis – An Update

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