Supercritical fluid chromatography coupled with tandem mass spectrometry: A high‐efficiency detection technique to quantify Taxane drugs in whole‐blood samples

Abstract

We present a technique to rapid determine taxane in blood samples by supercritical fluid chromatography together with mass spectrometry. The aim of this study was to develop a supercritical fluid chromatography with mass spectrometry method for the analysis of paclitaxel, cabazitaxel, and docetaxel in whole-blood samples of rats. Liquid-dry matrix spot extraction was selected in sample preparation procedure. Supercritical fluid chromatography separation of paclitaxel, cabazitaxel, docetaxel, and glyburide (internal standard) was accomplished within 3 min by using the gradient mobile phase consisted of methanol as the compensation solvent and carbon dioxide at a flow rate of 1.0 mL/min. The method was validated regarding specificity, the lower limit of quantification, repeatability, and reproducibility of quantification, extraction recovery, and matrix effects. The lower limit of quantification was found to be 10 ng/mL since it exhibited acceptable precision and accuracy at the corresponding level. All interday accuracies and precisions were within the accepted criteria of ±15% of the nominal value and within ±20% at the lower limit of quantification, implying that the method was reliable and reproducible. In conclusion, this method is a promising tool to support and improve preclinical or clinical pharmacokinetic studies with the taxanes anticancer drugs.

Application of Gold-Nanoparticle Colorimetric Sensing to Rapid Food Safety Screening

“…The main detection technologies for food quality and safety are instrumental analysis and rapid tests. The instrumental methods, such as gas chromatography [1], liquid chromatography [2], mass spectrometry (MS) [3], capillary electrophoresis [4], supercritical fluid chromatography [5], gas chromatography-MS [6], and liquid chromatography-MS [7], are undoubtedly powerful. All of these methods possess high sensitivity, good accuracy, and high stability, but they are often inconvenient, expensive, time-consuming, and require large, expensive analytical instruments and trained operators [8].…”

Development and optimization of a supercritical fluid chromatography tandem mass spectrometry method for the high‐throughput determination of nimodipine in beagle plasma

“…ACQUITY ultra‐performance convergence chromatography (UPC 2 ) is a significant and powerful analytical technology that is widely used in the separation and analysis of food and drugs . Compared with traditional LC, it is a low‐cost and environmentally friendly technique, especially suitable for non‐polar compound analysis that utilizes supercritical CO 2 as the primary mobile phase.…”