Supercritical Fluid Chromatography

Taylor, Larry T.

doi:10.1021/ac101194x

Cited by 117 publications

(56 citation statements)

References 79 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…8 This practice is still used often, as practitioners find use for materials designed for HILIC use (amide and aminopropyl bondings, etc.) and other polar phases, such as diols and poly(vinyl alcohol)s. 9 Chiral separations are ideally performed using SFC with specially designed phases, 10 which have even been successfully used for achiral separations. 11−14 Much work has been done to expand the separation space by using mobile phase additives with traditional stationary phases.…”

mentioning

confidence: 99%

Chromatographic Evidence of Silyl Ether Formation (SEF) in Supercritical Fluid Chromatography

et al. 2015

View full text Add to dashboard Cite

In this article, we propose that silyl ether formation (SEF) is a major contribution to retention and selectivity variation over time for supercritical fluid chromatography (SFC). In the past, the variations were attributed to instrumentation, but high performance SFC systems have shed new light on the source of variation. As silyl ethers form on the particle surface, the hydrophilicity is decreased, significantly altering the retention and selectivity observed. SEF is expected to occur with any chromatographic particle containing silanols but is slowed on hybrid inorganic/organic particles. The SEF reaction is between alcohols on the particle surface and in the mobile phase solvent. We have found that storage conditions of a column are paramount, which can either prevent or accelerate the process. Because SEF exists as an equilibrium between the liquid phase and the particle surface, the process is also reversible. The silanols can be hydroxylated (regenerated) to their original state upon exposure to water. The next generation of stationary phases will either advantageously utilize SEF or effectively mitigate its effects. Mitigation of SEF would be a significant improvement in SFC that has the potential to vault their performance to levels of similar reproducibility and reliability observed for high performance liquid chromatography (HPLC). Further research in SEF may lead to a better understanding of the mechanism of interaction between the solutes and chromatographic surface.

show abstract

mentioning

confidence: 99%

Chromatographic Evidence of Silyl Ether Formation (SEF) in Supercritical Fluid Chromatography

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Coupled with its ability to extract and produce value-added components, CO 2 has great potential for biorefinery applications that take advantage of its inherent properties to promote more efficient, greener processes. It is also worth mentioning that CO 2 may have other uses, such as its use as a working fluid (33) or in supercritical fluid chromatography (34), which are beyond the scope of this work, but are highly interesting fields of study. The research applications for the utilization of compressed CO 2 as a solvent are numerous and highly varied due to CO 2 's high versatility.…”

Section: Co 2 and The Biorefinerymentioning

confidence: 99%

Carbon Dioxide Solvent Applications in a Biorefinery

Soh

2014

ACS Symposium Series

View full text Add to dashboard Cite

“…SFC involves three types of gradient elution methods: temperature gradient such as GC, modifier programming such as LC, and pressure programming. In the same way, two types of analytical columns can be used, that is, packed columns such as HPLC and capillary columns such as GC (Taylor, 2010). Although, during the early days of this technology, SFC was widely expected to replace HPLC, nowadays, it is clear that HPLC continues playing a pivotal role in separation science; however, SFC is an attractive alternative for some applications such as chiral separation, fatty acids, triglycerides, and lipids analyses, or in pharmaceutical industry.…”

Section: Other Separation Techniquesmentioning

confidence: 99%

Strategies in the Analysis of Plant Flavonoids

Santos‐Buelga

Gonzaléz-Paramás

2014

Encyclopedia of Analytical Chemistry

View full text Add to dashboard Cite

Flavonoids are one of the largest groups of plant secondary metabolites. They comprise several thousand compounds that share a phenylchromane skeleton and can be classified into different classes, namely flavones, flavonols, flavanones, flavanols, anthocyanins, dihydroflavonols, isoflavones, and chalcones. Flavonoids occur in their natural sources as aglycones or glycosylated forms and as monomers or constituting polymerized structures and can be found both as free and matrix‐bound compounds. This structural diversity affects their physicochemical behavior, and different flavonoid classes and compounds may have different requisites for their extraction and analysis, so that there is not a unique analytical strategy that applies in all situations. In this chapter, the main methodological approaches to the analysis of flavonoids in plant materials are revised paying particular attention to more recent extraction techniques and high performance liquid chromatography‐based methodologies that currently dominate the field of flavonoid analysis.

show abstract

Supercritical Fluid Chromatography

Cited by 117 publications

References 79 publications

Chromatographic Evidence of Silyl Ether Formation (SEF) in Supercritical Fluid Chromatography

Chromatographic Evidence of Silyl Ether Formation (SEF) in Supercritical Fluid Chromatography

Carbon Dioxide Solvent Applications in a Biorefinery

Strategies in the Analysis of Plant Flavonoids

Contact Info

Product

Resources

About