Supercritical CO2 technology for one-pot foaming and sterilization of polymeric scaffolds for bone regeneration

Santos‐Rosales, Víctor; Magariños, Beatríz; Starbird, Ricardo; Suárez-González, Javier; Fariña, José B.; García‐González, Carlos A.

doi:10.1016/j.ijpharm.2021.120801

Cited by 19 publications

(10 citation statements)

References 56 publications

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“…On the one hand, FDA regulatory criteria have put a maximum 0.5 EU/ml threshold of endotoxin contamination for medical devices, including dECM scaffolds [75]. On the other hand, implantable medical devices are not allowed to have pathogen contamination with a probability of higher than one in a million [216]. Accordingly, the utilization of suitable sterilization techniques in manufacturing dECM scaffolds is of the highest importance to avoid infection and rejection [217].…”

Section: Sterilization To Eliminate Pathogen-related Immunogenicity O...mentioning

confidence: 99%

Immunogenicity of decellularized extracellular matrix scaffolds: a bottleneck in tissue engineering and regenerative medicine

et al. 2023

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Tissue-engineered decellularized extracellular matrix (ECM) scaffolds hold great potential to address the donor shortage as well as immunologic rejection attributed to cells in conventional tissue/organ transplantation. Decellularization, as the key process in manufacturing ECM scaffolds, removes immunogen cell materials and significantly alleviates the immunogenicity and biocompatibility of derived scaffolds. However, the application of these bioscaffolds still confronts major immunologic challenges. This review discusses the interplay between damage-associated molecular patterns (DAMPs) and antigens as the main inducers of innate and adaptive immunity to aid in manufacturing biocompatible grafts with desirable immunogenicity. It also appraises the impact of various decellularization methodologies (i.e., apoptosis-assisted techniques) on provoking immune responses that participate in rejecting allogenic and xenogeneic decellularized scaffolds. In addition, the key research findings regarding the contribution of ECM alterations, cytotoxicity issues, graft sourcing, and implantation site to the immunogenicity of decellularized tissues/organs are comprehensively considered. Finally, it discusses practical solutions to overcome immunogenicity, including antigen masking by crosslinking, sterilization optimization, and antigen removal techniques such as selective antigen removal and sequential antigen solubilization.

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Section: Sterilization To Eliminate Pathogen-related Immunogenicity O...mentioning

confidence: 99%

Immunogenicity of decellularized extracellular matrix scaffolds: a bottleneck in tissue engineering and regenerative medicine

et al. 2023

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“…While discussing the crucial properties of a scaffold for bone tissue regeneration, it is mandatory to highlight: (1) biocompatibility, or biomimetic properties [ 28 , 44 , 45 ], (2) mechanical properties [ 22 , 46 ] and (3) biodegradability [ 47 , 48 ]. Some authors among the important requirements for an ideal scaffold emphasize the porosity and pore size [ 49 ], cell attachment properties [ 46 , 50 ], ability to visualize, histologically investigate, and sterilize the tissue-engineered construct [ 51 , 52 , 53 , 54 ].…”

Section: Recent Developments In Bone Tissue Engineeringmentioning

confidence: 99%

In Vivo Bone Tissue Engineering Strategies: Advances and Prospects

et al. 2022

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Reconstruction of critical-sized bone defects remains a tremendous challenge for surgeons worldwide. Despite the variety of surgical techniques, current clinical strategies for bone defect repair demonstrate significant limitations and drawbacks, including donor-site morbidity, poor anatomical match, insufficient bone volume, bone graft resorption, and rejection. Bone tissue engineering (BTE) has emerged as a novel approach to guided bone tissue regeneration. BTE focuses on in vitro manipulations with seed cells, growth factors and bioactive scaffolds using bioreactors. The successful clinical translation of BTE requires overcoming a number of significant challenges. Currently, insufficient vascularization is the critical limitation for viability of the bone tissue-engineered construct. Furthermore, efficacy and safety of the scaffolds cell-seeding and exogenous growth factors administration are still controversial. The in vivo bioreactor principle (IVB) is an exceptionally promising concept for the in vivo bone tissue regeneration in a predictable patient-specific manner. This concept is based on the self-regenerative capacity of the human body, and combines flap prefabrication and axial vascularization strategies. Multiple experimental studies on in vivo BTE strategies presented in this review demonstrate the efficacy of this approach. Routine clinical application of the in vivo bioreactor principle is the future direction of BTE; however, it requires further investigation for to overcome some significant limitations.

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“…The results draw attention because gamma radiation is a common tool used for sterilization [1] and it may change cellulose properties including viscosity [2] , mechanical and barrier properties [3] , molecular weight [4] , surface area [5] and crystallinity [5] . Accordingly, the gamma effect on the physical and chemical properties may provide a feasible method to tailor the properties of the cellulose for the particular application [6] . The experimental conditions and radiation dosage induce scission or crosslinking reactions in polymers during the ionizing treatment [7] .…”

Section: Data Descriptionmentioning

confidence: 99%

Characterization data of cellulose modified by gamma irradiation to be used as template in the synthesis of a photoactive composite for environmental applications

et al. 2022

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Supercritical CO2 technology for one-pot foaming and sterilization of polymeric scaffolds for bone regeneration

Cited by 19 publications

References 56 publications

Immunogenicity of decellularized extracellular matrix scaffolds: a bottleneck in tissue engineering and regenerative medicine

Immunogenicity of decellularized extracellular matrix scaffolds: a bottleneck in tissue engineering and regenerative medicine

In Vivo Bone Tissue Engineering Strategies: Advances and Prospects

Characterization data of cellulose modified by gamma irradiation to be used as template in the synthesis of a photoactive composite for environmental applications

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