Supercritical CO2 foamed composite scaffolds incorporating bioactive lipids promote vascularized bone regeneration via Hif-1α upregulation and enhanced type H vessel formation

Li, Shuang; Song, Chaobo; Yang, Shengbing; Yu, Weijun; Zhang, Weiqi; Zhang, Guohua; Xi, Zhenhao; Lu, Eryi

doi:10.1016/j.actbio.2019.05.066

Cited by 56 publications

(46 citation statements)

References 76 publications

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“…PLGA has been used in locally implanted medical devices, including scaffolds for controlled drug release and to enhance drug bioavailability in tissue repairing processes [ 15 ]. The studies conducted have achieved the impregnation of gemcitabine in PLGA foams from ethyl lactate solutions of gemcitabine [ 16 ], mesoporous bioactive glass particles (MBGs) have been incorporated into PLGA [ 17 ], PLGA composite foams were produced using phosphate glass particles as filler [ 18 ], thymol has been impregnated into PLGA for controlled release [ 19 ] and bioactive lipids have been incorporated to PLGA scaffolds [ 20 ]. In some cases, such as bone tissue repair, the action of the drug is to be prolonged for as long as months.…”

Section: Introductionmentioning

confidence: 99%

Determining the Optimal Conditions for the Production by Supercritical CO2 of Biodegradable PLGA Foams for the Controlled Release of Rutin as a Medical Treatment

et al. 2021

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Poly(D,L,-lactide-co-glycolide) (PLGA) foam samples impregnated with rutin were successfully produced by supercritical foaming processes. A number of parameters such as pressure (80–200 bar), temperature (35–55 °C), depressurization rate (5–100 bar/min), ratio lactide:glycolide of the poly(D,L,-lactide-co-glycolide) (50:50 and 75:25) were studied to determine their effect on the expansion factor and on the glass transition temperature of the polymer foams and their consequences on the release profile of the rutin entrapped in them. The impregnated foams were characterized by scanning electron microscopy, differential scanning calorimetry, and mercury intrusion porosimetry. A greater impregnation of rutin into the polymer foam pores was observed as pressure was increased. The release of rutin in a phosphate buffer solution was investigated. The controlled release tests confirmed that the modification of certain variables would result in considerable differences in the drug release profiles. Thus, five-day drug release periods were achieved under high pressure and temperature while the depressurization rate remained low.

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Section: Introductionmentioning

confidence: 99%

Determining the Optimal Conditions for the Production by Supercritical CO2 of Biodegradable PLGA Foams for the Controlled Release of Rutin as a Medical Treatment

et al. 2021

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“…The cells were fixed and stained with 1% Alizarin red solution (Cyagen, Guangzhou, China). For the quantitation of calcium deposition, Alizarin red binding with calcium nodules was desorbed by 10% cetylpyridinium chloride (Aladdin, Shanghai, China), and OD measurements were obtained at a wavelength of 570 nm ( Li et al, 2019 ).…”

Section: Methodsmentioning

confidence: 99%

Macrophage-Mediated Bone Formation in Scaffolds Modified With MSC-Derived Extracellular Matrix Is Dependent on the Migration Inhibitory Factor Signaling Pathway

Deng

Tan

Dai

et al. 2021

Front. Cell Dev. Biol.

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The positive role of macrophages in the osteogenesis of mesenchymal stem cells (MSCs) has been a recent research focus. On the other hand, MSCs could carefully regulate the paracrine molecules derived from macrophages. Human umbilical cord mesenchymal stem cells (hucMSCs) can reduce the secretion of inflammatory factors from macrophages to improve injury healing. hucMSC-derived extracellular matrix (hucMSC-ECM) has the similar effect to hucMSCs, which could combat the inflammatory response of macrophages. Additionally, MSC-derived extracellular matrix also enhanced bone regeneration by inhibiting osteoclastic differentiation of monocyte/macrophage lineage. However, whether hucMSC-ECM could improve bone formation by guiding macrophage-induced osteogenic differentiation of MSCs is unknown. Here, we present decalcified bone scaffolds modified by hucMSC-derived extracellular matrix (DBM-ECM), which maintained multiple soluble cytokines from hucMSCs, including macrophage migration inhibitory factor (MIF). Compared with DBM, the DBM-ECM scaffolds induced bone formation in an improved heterotopic ossification model of severe combined immunodeficiency (SCID) mice in a macrophage-dependent manner. Macrophages cocultured with DBM-ECM expressed four osteoinductive cytokines (BMP2, FGF2, TGFβ3 and OSM), which were screened out by RNA sequencing and measured by qPCR and western blot. The conditioned medium from macrophages cocultured with DBM-ECM improved the osteogenic differentiation of hBMSCs. Furthermore, DBM-ECM activated CD74/CD44 (the typical MIF receptors) signal transduction in macrophages, including phosphorylation of P38 and dephosphorylation of c-jun. On the other side, the inhibitory effects of the DBM-ECM scaffolds with a deficient of MIF on osteogenesis in vitro and in vivo revealed that macrophage-mediated osteogenesis depended on MIF/CD74 signal transduction. The results of this study indicate that the coordinated crosstalk of macrophages and MSCs plays a key role on bone regeneration, with an emphasis on hucMSC-ECM constructing a macrophage-derived osteoinductive microenvironment.

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“…CO 2 foamed composite scaffold incorporating bioactive lipids promotes vascularization and bone regeneration via HIF1‐α upregulation and enhanced type H vessel formation. ( 211 ) Mechanobiologically optimized magnesium (Mg) scaffolds (CaP‐coated Mg‐Zn‐Gd scaffolds) enhance the ratio of type H ECs and osteogenic differentiation of bone mesenchymal stem cells (BMSCs) during repair of orbital bone defects. ( 212 ) Moreover, deferoxamine‐loaded 3D‐printed biodegradable scaffold fabricated via surface aminolysis and layer‐by‐layer assembly technique shows enhanced HIF1‐α, CD31 high expression ECs, and osteogenesis.…”

Section: Angiogenesis and Vascularization In Bone Tissue Engineeringmentioning

confidence: 99%

Bone Vasculature and Bone Marrow Vascular Niches in Health and Disease

Chen

Hendriks

Chatzis

et al. 2020

Journal of Bone and Mineral Research

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Bone vasculature and bone marrow vascular niches supply oxygen, nutrients, and secrete angiocrine factors required for the survival, maintenance, and self-renewal of stem and progenitor cells. In the skeletal system, vasculature creates nurturing niches for bone and blood-forming stem cells. Blood vessels regulate hematopoiesis and drive bone formation during development, repair, and regeneration. Dysfunctional vascular niches induce skeletal aging, bone diseases, and hematological disorders. Recent cellular and molecular characterization of the bone marrow microenvironment has provided unprecedented insights into the complexity, heterogeneity, and functions of the bone vasculature and vascular niches. The bone vasculature is composed of distinct vessel subtypes that differentially regulate osteogenesis, hematopoiesis, and disease conditions in bones. Further, bone marrow vascular niches supporting stem cells are often complex microenvironments involving multiple different cell populations and vessel subtypes. This review provides an overview of the emerging vascular cell heterogeneity in bone and the new roles of the bone vasculature and associated vascular niches in health and disease.

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Supercritical CO2 foamed composite scaffolds incorporating bioactive lipids promote vascularized bone regeneration via Hif-1α upregulation and enhanced type H vessel formation

Cited by 56 publications

References 76 publications

Determining the Optimal Conditions for the Production by Supercritical CO2 of Biodegradable PLGA Foams for the Controlled Release of Rutin as a Medical Treatment

Determining the Optimal Conditions for the Production by Supercritical CO2 of Biodegradable PLGA Foams for the Controlled Release of Rutin as a Medical Treatment

Macrophage-Mediated Bone Formation in Scaffolds Modified With MSC-Derived Extracellular Matrix Is Dependent on the Migration Inhibitory Factor Signaling Pathway

Bone Vasculature and Bone Marrow Vascular Niches in Health and Disease

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