Supercritical CO2 extraction to obtain Lippia thymoides Mart. &amp; Schauer (Verbenaceae) essential oil rich in thymol and evaluation of its antimicrobial activity

Silva, Sebastião Gomes; Oliveira, Mozaniel Santana de; Cruz, Jorddy Neves; Costa, Wanessa Almeida da; Silva, Sílvia Helena Marques da; Maia, Anabela; Sousa, Ronaldo Lopes de; Carvalho, Raul Nunes de; Andrade, Eloísa Helena de Aguiar

doi:10.1016/j.supflu.2020.105064

Cited by 37 publications

(23 citation statements)

References 55 publications

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“…These techniques depend on the part of the plants from which the oil will be extracted, the stability of the oil when faced with heat, and the susceptibility of the oil's constituents to undergoing chemical reactions. Some of the techniques commonly used for EO extraction are hydrodistillation, hydrodiffusion, enfleurage, cold pressing, steam distillation, solvent extraction, microwave-assisted process, and carbon dioxide extraction [16][17][18][19][20][21].…”

Section: Essential Oilsmentioning

confidence: 99%

Essential Oils from Annonaceae Species from Brazil: A Systematic Review of Their Phytochemistry, and Biological Activities

Cascaes

Carneiro

Nascimento

et al. 2021

IJMS

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The present work involves a systematic review of the chemical composition and biological effects of essential oils from the Annonaceae species collected in Brazil from 2011 to 2021. Annonaceae is one of the most important botanical families in Brazil, as some species have economic value in the market as local and international fruit. In addition, the species have useful applications in several areas—for instance, as raw materials for use in cosmetics and perfumery and as medicinal plants. In folk medicine, species such as Annona glabra L. and Xylopia sericea A. St.-Hil. are used to treat diseases such as rheumatism and malaria. The species of Annonaceae are an important source of essential oils and are rich in compounds belonging to the classes of mono and sesquiterpenes; of these compounds, α-pinene, β-pinene, limonene, (E)-caryophyllene, bicyclogermacrene, caryophyllene oxide, germacrene D, spathulenol, and β-elemene are the most abundant. The antimicrobial, anti-inflammatory, antileishmania, antioxidant, antiproliferative, cytotoxic, larvicidal, trypanocidal, and antimalarial activities of essential oils from the Annonaceae species in Brazil have been described in previous research, with the most studies on this topic being related to their antiproliferative or cytotoxic activities. In some studies, it was observed that the biological activity reported for these essential oils was superior to that of drugs available on the market, as is the case of the essential oil of the species Guatteria punctata (Aubl.) R. A. Howard., which showed a trypanocidal effect that was 34 times stronger than that of the reference drug benznidazol.

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Section: Essential Oilsmentioning

confidence: 99%

Essential Oils from Annonaceae Species from Brazil: A Systematic Review of Their Phytochemistry, and Biological Activities

Cascaes

Carneiro

Nascimento

et al. 2021

IJMS

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“…The chemical compositions of the EOs of E. florida (A and B), were analyzed using a Shimadzu QP-2010 plus (Kyoto, Japan) a gas chromatography system equipped with an Rtx-5MS capillary column (30 m × 0.25 mm; 0.25 µm film thickness) (Restek Corporation, Bellefonte, PA, USA) coupled to a mass spectrometer (GC/MS) (Shimadzu, Kyoto, Japan). The program temperature was maintained at 60-240 • C at a rate of 3 • C/min, with an injector temperature of 250 • C, helium as the carrier gas (linear velocity of 32 cm/s, measured at 100 • C) and a splitless injection (1 µL of a 2:1000 hexane solution), using the same operating conditions as described in the literature [67,68]. Except for the carrier hydrogen gas, the components were quantified using gas chromatography (CG) on a Shimadzu QP-2010 system (Kyoto, Japan), equipped with a flame ionization detector (FID) (Kyoto, Japan), under the same operating conditions as before.…”

Section: Chemical Composition Analysismentioning

confidence: 99%

Chemical Composition and Antioxidant Activity of Essential Oils from Leaves of Two Specimens of Eugenia florida DC.

et al. 2021

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Eugenia florida DC. belongs to the Myrtaceae family, which is present in almost all of Brazil. This species is popularly known as pitanga-preta or guamirim and is used in folk medicine to treat gastrointestinal problems. In this study, two specimens of Eugenia florida (Efl) were collected in different areas of the same region. Specimen A (EflA) was collected in an area of secondary forest (capoeira), while specimen B (EflB) was collected in a floodplain area. The essential oils (EOs) were extracted from both specimens of Eugenia florida by means of hydrodistillation. Gas chromatography coupled to mass spectrometry (GC/MS) was used to identify the volatile compounds present, and the antioxidant capacity of the EOs was determined by antioxidant capacity (AC-DPPH) and the Trolox equivalent antioxidant (TEAC) assay. For E. florida, limonene (11.98%), spathulenol (10.94%) and α-pinene (5.21%) were identified as the main compounds of the EO extracted from sample A, while sample B comprised selina-3,11-dien-6α-ol (12.03%), eremoligenol (11.0%) and γ-elemene (10.70%). This difference in chemical composition impacted the antioxidant activity of the EOs between the studied samples, especially in sample B of E. florida. This study is the first to report on the antioxidant activity of Eugenia florida DC. essential oils.

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“…The chemical compositions of the EOs of E. patrisii, E. punicifolia, and M. tomentosa, were analyzed using a Shimadzu QP-2010 (Kyoto, Japan) plus gas chromatography system equipped with an Rtx-5MS capillary column (Restek Corporation, Bellefonte, PA USA) (30 m × 0.25 mm; 0.25 µm film thickness) coupled to a mass spectrometer (GC/MS) (Shimadzu, Kyoto, Japan). The program temperature was maintained at 60-240 • C at a rate of 3 • C/min, with an injector temperature of 250 • C, helium as the carrier gas (linear velocity of 32 cm/s, measured at 100 • C) and a splitless injection (1 µL of a 2:1000 hexane solution) using the same operating conditions as described in the literature [59,60]. Except for the carrier hydrogen gas, the components were quantified using gas chromatography (CG) on a Shimadzu QP-2010 system (Kyoto, Japan), equipped with a flame ionization detector (FID), under the same operating conditions as before.…”

Section: Chemical Composition Analysismentioning

confidence: 99%

Chemical Composition and Antioxidant Activity of Essential Oils from Eugenia patrisii Vahl, E. punicifolia (Kunth) DC., and Myrcia tomentosa (Aubl.) DC., Leaf of Family Myrtaceae

et al. 2021

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Essential oils (EOs) were extracted from Eugenia patrisii, E. punicifolia, and Myrcia tomentosa, specimens A and B, using hydrodistillation. Gas chromatography coupled with mass spectrometry (GC/MS) was used to identify the volatile constituents present, and the antioxidant capacity of EOs was determined using diphenylpicryl-hydrazyl (DPPH) and trolox equivalent antioxidant capacity (TEAC) assays. For E. patrisii, germacrene D (20.03%), bicyclogermacrene (11.82%), and (E)-caryophyllene (11.04%) were identified as the major constituents of the EOs extracted from specimen A, whereas specimen B primarily comprised γ-elemene (25.89%), germacrene B (8.11%), and (E)-caryophyllene (10.76%). The EOs of E. punicifolia specimen A contained β-Elemene (25.12%), (E)-caryophyllene (13.11%), and bicyclogermacrene (9.88%), while specimen B was composed of (E)-caryophyllene (11.47%), bicyclogermacrene (5.86%), β-pinene (5.86%), and γ-muurolene (5.55%). The specimen A of M. tomentosa was characterized by γ-elemene (12.52%), germacrene D (11.45%), and (E)-caryophyllene (10.22%), while specimen B contained spathulenol (40.70%), α-zingiberene (9.58%), and γ-elemene (6.89%). Additionally, the chemical composition of the EOs was qualitatively and quantitatively affected by the collection period. Furthermore, the EOs of the studied specimens, especially specimen A of E. punicifolia, showed a greater antioxidant activity in DPPH rather than TEAC, as represented by a significantly high inhibition percentage (408.0%).

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Supercritical CO2 extraction to obtain Lippia thymoides Mart. & Schauer (Verbenaceae) essential oil rich in thymol and evaluation of its antimicrobial activity

Cited by 37 publications

References 55 publications

Essential Oils from Annonaceae Species from Brazil: A Systematic Review of Their Phytochemistry, and Biological Activities

Essential Oils from Annonaceae Species from Brazil: A Systematic Review of Their Phytochemistry, and Biological Activities

Chemical Composition and Antioxidant Activity of Essential Oils from Leaves of Two Specimens of Eugenia florida DC.

Chemical Composition and Antioxidant Activity of Essential Oils from Eugenia patrisii Vahl, E. punicifolia (Kunth) DC., and Myrcia tomentosa (Aubl.) DC., Leaf of Family Myrtaceae

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