Supercritical CO2 extraction of carotenoids and other lipids from Chlorella vulgaris

Mendes, Rui L.; Fernandes, Helena; Coelho, José Augusto Paixão; Reis, Elsa C.; Cabral, Joaquim M. S.; Novais, J. M.; Palavra, A. M. F.

doi:10.1016/0308-8146(95)95794-7

Cited by 195 publications

(128 citation statements)

References 11 publications

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“…The amount of lutein dramatically increased during the first 30 minutes of extraction time and then continued to increase gradually to about 45 minutes, a point which offers the most efficient extractable amount of lutein of around 1.78 ± 0.01 % at 40 MPa; only a small increase in lutein amount was observed after 45 minutes of extraction time. The high lutein extraction rate achieved at the initial extraction was due to the high driving force of lutein mass transfer between the inside and outside the algal cell [8,13]. Fig.…”

Section: Resultsmentioning

confidence: 97%

Selective Extraction of Lutein from Alcohol Treated Chlorella vulgaris by Supercritical CO₂

et al. 2012

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A pretreatment process using alcohol for the removal of chlorophyll a, b and b-carotene from Chlorella vulgaris was developed to improve the yield and selectivity of lutein in the extract obtained by supercritical carbon dioxide extraction. Supercritical carbon dioxide extraction was carried out after pretreatment in the pressure range of 20 to 40 MPa and the temperature range of 40 to 80°C. Ethanol and methanol were selected as elution solvents, of which ethanol was found most suitable for the elution, or pretreatment, process. The amounts of lutein and other compounds were analyzed by HPLC with the mixture of methanol and THF as the mobile phase. The amount of lutein in the extract increased with pressure, but decreased with extraction temperature. The highest recovery percentage and the selectivity of lutein were around 52.9 ± 0.02 % and 43.1 ± 0.02 %, respectively, obtained from supercritical carbon dioxide extraction with pretreatment and ethanol entrainer at 40 MPa and 40°C.

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Section: Resultsmentioning

confidence: 97%

Selective Extraction of Lutein from Alcohol Treated Chlorella vulgaris by Supercritical CO₂

et al. 2012

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“…The extraction and fractionation of fats (Bhaskar et al 1993 ;Buening-Pfaue et al 1993) and of volatile oil from various materials (Vuorela et al 1990 ;Revechon et al 1994), the removal of cholesterol from beef (Wehling et al 1992), milk fat (Bradley 1989) and egg yolk (Froning et al 1990) have been investigated. The extraction of natural pigments from plant materials (Spanos et al 1993 ;Mendes et al 1995), and of the Ñavour from hops and spices (Huber and Vitzthun 1980 ;Sharpe and Crab 1980) have also been reported in literature. The deca †eination of co †ee (Zosel 1989) has already been developed in the food industry.…”

Section: Introductionmentioning

confidence: 94%

Study on the pilot-scale extraction of onion oleoresin using supercritical CO2

Sass-Kiss

Simándi

Gao

et al. 1998

J. Sci. Food Agric.

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“…The main constituents of the lipid fraction of the fresh water microalga Chlorella vulgaris are oleic (18:1 n-9), palmitic (16:0) and linolenic (18:3 n-3) acids, accounting for 41, 22 and 9% of the total amount, respectively (Mendes et al, 1995). In another species, Dunaliella salina, these fatty acids account for more than 80% of the total of fatty acids (Herrero et al, 2006).…”

Section: Microalgae As Lipid and Pufa Sourcesmentioning

confidence: 99%

Marine microalgae used as food supplements and their implication in preventing cardiovascular diseases

Mimouni

Ulmann

Haimeur

et al. 2015

OCL

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-Marine microalgae are photosynthetic microorganisms producing numerous bioactive molecules of interest for health and disease care such as lipids rich in omega-3 fatty acids -as eicosapentaenoic acid (EPA, 20:5 n-3) and docosahexaenoic acid (DHA, 22:6 n-3)-and carotenoids (e.g., β-carotene, fucoxanthin, astaxanthin). It has already been shown that these molecules, individually used, are benefic in the prevention of diseases such as those associated with the cardiovascular risks, but also in some carcinomas. When these molecules are combined, synergistic effects may be observed. Microalgae, as a dietary supplement, can be used to study these synergistic effects in animal models in which dyslipidemia can be induced by a nutrition treatment. Different marine microalgae of interest are studied in this context to determine their potential effect as an alternative source to marine omega-3 rich fish oils, actually widely used for human health. Actually, the pharmaceutical and nutrition industries are developing health research programs involving microalgae, trying to limit the dramatic reduction of fish stocks and the associated pollution in the marine environment. The aim of this review is threefold: (1) to present research on lipids, particularly long chain polyunsaturated fatty acids, as components of marine microalgae used as food supplements; (2) to present the health benefits of some microalgae or their extracts, in particular in the prevention of cardiovascular diseases and (3) to highlight the role of Odontella aurita, a marine microalga rich in EPA used as food supplement with the aim of preventing cardiovascular diseases.Keywords: Microalgae / Odontella aurita / omega-3 polyunsaturated fatty acids / food supplement / cardiovascular diseases Résumé -Microalgues marines utilisées comme compléments alimentaires et leur rôle dans la prévention des maladies cardiovasculaires. Les microalgues marines sont des micro-organismes photosynthétiques produisant de nombreuses molécules bioactives d'intérêt dans le domaine de la santé, tels que des lipides riches en acides gras oméga-3, comme l'acide eicosapentaénoïque (EPA, 20 :5 n-3) et l'acide docosahexaénoïque (DHA, 22 :6, n-3), et des caroténoïdes (par exemple, le β-carotène, la fucoxanthine, l'astaxanthine). Il a déjà été démontré que ces molécules ont un effet positif dans la prévention de maladies notamment celles qui sont associées au système cardiovasculaire, mais aussi dans certains carcinomes. Lorsque ces molécules sont combinées, des effets synergiques peuvent être observés. Les microalgues, utilisées en tant que compléments alimentaires, peuvent contribuer à étudier les effets synergiques de ces composés chez des modèles animaux pour lesquels apparaît une dyslipidémie d'origine nutritionnelle. Différentes microalgues marines sont utilisées dans ce contexte afin de déterminer leur potentiel d'action et de rechercher une source de lipides alternative aux huiles de poissons, ces dernières étant largement utilisées dans le domaine de la santé humaine en ...

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Supercritical CO2 extraction of carotenoids and other lipids from Chlorella vulgaris

Cited by 195 publications

References 11 publications

Selective Extraction of Lutein from Alcohol Treated Chlorella vulgaris by Supercritical CO₂

Selective Extraction of Lutein from Alcohol Treated Chlorella vulgaris by Supercritical CO₂

Study on the pilot-scale extraction of onion oleoresin using supercritical CO2

Marine microalgae used as food supplements and their implication in preventing cardiovascular diseases

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Supercritical CO2 extraction of carotenoids and other lipids from Chlorella vulgaris

Cited by 195 publications

References 11 publications

Selective Extraction of Lutein from Alcohol Treated Chlorella vulgaris by Supercritical CO2

Selective Extraction of Lutein from Alcohol Treated Chlorella vulgaris by Supercritical CO2

Study on the pilot-scale extraction of onion oleoresin using supercritical CO2

Marine microalgae used as food supplements and their implication in preventing cardiovascular diseases

Contact Info

Product

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Selective Extraction of Lutein from Alcohol Treated Chlorella vulgaris by Supercritical CO₂

Selective Extraction of Lutein from Alcohol Treated Chlorella vulgaris by Supercritical CO₂