Supercritical CO2: A novel environmentally friendly mutagen

Zhang, Qiao Y.; Qian, Junqing; Guo, Hui; Yang, Sheng

doi:10.1016/j.mimet.2008.04.007

Cited by 8 publications

(7 citation statements)

References 19 publications

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“…Although 0.5% HZH alone resulted in mutagenicity, it brought lower positive mutation rate (only 8.3%) than the case of HZH in supercritical CO 2 . Perhaps supercritical CO 2 facilitated transient permeability and induced mutation function of the additive (Zhang et al 2008). Other authors have demonstrated that the activity of some intracellular enzymes declined, or lost after supercritical treatment such as ATPase, phosphatase, peroxidase, etc (Bertoloni et al 2006;Gui et al 2006;Liu et al 2008).…”

Section: Resultsmentioning

confidence: 99%

“…The corresponding positive mutation rate was 45.6%. A mutant strain with about 44.2% increase in lipase yield than the wild strain was obtained by supercritical CO 2 and 1.0% DMSO (Zhang et al 2008). In order to obtain a mutant strain with more productive lipase, a better substitute for DMSO was considered to be explored.…”

Section: Resultsmentioning

confidence: 99%

“…It is known that stress is a disturbance to the normal functioning of a biological system and mutagenesis is often increased in the bacterial populations as a consequence of stress-induced genetic pathways (Tenaillon et al 2004;Foster 2005). A previous study had demonstrated that supercritical CO 2 was a promising environmentally-friendly mutagen, or co-mutagen owing to its distinctive stress (Zhang et al 2008). Carbon dioxide (CO 2 ) is an inert, inexpensive, easily available, odorless, tasteless, environmentfriendly, and GRAS (Generally Recognized As Safe) solvent.…”

Section: Introductionmentioning

confidence: 99%

“…CO 2 is frequently used in near-, or supercritical conditions, wherein it shows excellent mass transfer properties in terms of penetration into matrices (Valverde et al 2010). Possibly due to assist in the permeability, supercritical CO 2 had been found to enhance the sterilization effects and/or mutagenicity of the additives (co-solvents) such as hydrogen peroxide (H 2 O 2 ) (Zhang et al 2006), dimethylsulfoxide (DMSO) (Zhang et al 2008), etc. The solvent properties of supercritical CO 2 could also be drastically changed by the addition of a small amount of polar solvent as a modifier (Sicardi et al 2000).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Mutation breeding of lipase-producing strain Flavobacterium sp. by supercritical CO2 with hydrazine hydrate

Zhang

Qian

2013

Braz. arch. biol. technol.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Mutation breeding of lipase-producing strain Flavobacterium sp. by supercritical CO2 with hydrazine hydrate

Zhang

Qian

2013

Braz. arch. biol. technol.

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“…The application of non-thermal sterilization technologies has been increased recently in food and pharmaceutical industries due to its inactivation efficiency, non-toxic and inflammable [45][46][47]. The efficiency of SC-CO 2 for inactivating of fungi in different media such as culture media, physiological saline was demonstrated by several authors [48][49][50][51][52][53].…”

Section: Inactivation Of Fungal Spores By Sc-comentioning

confidence: 99%

Inactivation of Aspergillus Spores in Clinical Wastes by Supercritical Carbon Dioxide

Efaq

Rahman²,

Nagao³

et al. 2016

Arab J Sci Eng

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The present work investigated the inactivation of Aspergillus spp. spores by supercritical carbon dioxide (SC-CO 2 ) based on the destruction of enzyme activity and the alteration of spore morphology as documented using a scanning electron microscope (SEM). Six Aspergillus spp. included A. niger, Aspergillus sp. in section Nigri, A. fumigatus, A. tubingensis, A. hortai and Aspergillus sp. strain no 145 were isolated from clinical waste samples and inactivated by using SC-CO 2 at optimal conditions (35 MPa, 75 • C and 90 min). The inactivation of spores was determined by the culturing method. Aspergillus sp. strain no 145 and Aspergillus hortai were further analysed to quantify enzyme activity, and the morphology of spores was determined by SEM to demonstrate the inactivation mechanism. The results revealed that growth and enzyme activity of the SC-CO 2 treated fungal spores were not detected. The SEM observation demonstrated complete destruction of spore morphology and exsorption of protoplasmic contents. The findings proved the high efficiency of SC-CO 2 for the destruction of fungal contaminants in clinical wastes. B A. N. Efaq

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Microbial production of metabolites and associated enzymatic reactions under high pressure

Dong

Jiang

2016

World J Microbiol Biotechnol

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High environmental pressure exerts an external stress on the survival of microorganisms that are commonly found under normal pressure. In response, many growth traits alter, including cell morphology and physiology, cellular structure, metabolism, physical and chemical properties, the reproductive process, and defense mechanisms. The high-pressure technology (HP) has been industrially utilized in pressurized sterilization, synthesis of stress-induced products, and microbial/enzymatic transformation of chemicals. This article reviews current research on pressure-induced production of metabolites in normal-pressure microbes and their enzymatic reactions. Factors that affect the production of such metabolites are summarized, as well as the effect of pressure on the performance of microbial fermentation and the yield of flavoring compounds, different categories of induced enzymatic reactions and their characteristics in the supercritical carbon dioxide fluid, effects on enzyme activity, and the selection of desirable bacterial strains. Technological challenges are discussed, and future research directions are proposed. Information presented here will benefit the research, development, and application of the HP technology to improve microbial fermentation and enzymatic production of biologically active substances, thereby help to meet their increasing demand from the ever-expanding market.

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Supercritical CO2: A novel environmentally friendly mutagen

Cited by 8 publications

References 19 publications

Mutation breeding of lipase-producing strain Flavobacterium sp. by supercritical CO2 with hydrazine hydrate

Mutation breeding of lipase-producing strain Flavobacterium sp. by supercritical CO2 with hydrazine hydrate

Inactivation of Aspergillus Spores in Clinical Wastes by Supercritical Carbon Dioxide

Microbial production of metabolites and associated enzymatic reactions under high pressure

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