Supercritical CO<sub>2</sub> extraction of pigment components with pharmaceutical importance from <i>Chlorella vulgaris</i>

Kitada, Kiwa; Machmudah, Siti; Sasaki, Mitsuru; Goto, Motonobu; Nakashima, Yuya; Kumamoto, Shoichiro; Hasegawa, Takashi

doi:10.1002/jctb.2096

Cited by 104 publications

(82 citation statements)

References 19 publications

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“…Generally, the yield of extract influenced by the increase of pressure or temperature at constant temperature or pressure when SCCO2 was applied as an extraction medium. This phenomena occurred due to direct change of density and hence solubility of SCCO2 [22,23]. In this work, it should be noted that the lipid extraction by SCCO2 was performed at pressure of 40 MPa with various extraction temperatures.…”

Section: Fatty Acid Profilementioning

confidence: 97%

Economical Wet Extraction of Lipid from labyrinthula Aurantiochytrium limacinum by Using Liquefied Dimethyl Ether

Hoshino

Murakami

Wahyudiono

et al. 2016

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Abstract. Recently, a simple method for the extraction of lipids from wet biomass using liquefied dimethyl ether (DME) without drying, cell disruption, or heating was proposed. Here, the versatility of this method was evaluated for labyrinthula Aurantiochytrium limacinum (A. limacinum). The liquefied DME was passed through the extractor that filled by A. limacinum at different time intervals. The extraction of lipids from A. limacinum of moisture-rich microorganism was successfully achieved, the yield of lipid was 46.1 wt% of the dry weight of the sample. In comparison, the yields of lipid were 21.3 wt%, 43.6 wt% and 50.7 wt% when supercritical carbon dioxide (SCCO2), hexane-Soxhlet and Bligh-Dyer (BD) extraction methods were applied as extractants, respectively. However, the drying and cell-disruption process were required in SCCO2, hexane-Soxhlet, and BD extraction methods.

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Section: Fatty Acid Profilementioning

confidence: 97%

Economical Wet Extraction of Lipid from labyrinthula Aurantiochytrium limacinum by Using Liquefied Dimethyl Ether

Hoshino

Murakami

Wahyudiono

et al. 2016

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“…In the present work, supercritical carbon dioxide (SCCO2) was employed as a solvent extraction [10][11][12][13][14] to extract phenolic compounds from grains of paradise seeds. The SCCO2 extraction technique is very advantageous and environmentally friendly compared with other conventional extraction technique, such as soxhlet extraction technique.…”

Section: Introductionmentioning

confidence: 99%

Extraction of Phytochemicals from Grains of Paradise Using Supercritical Carbon Dioxide

Ono¹,

Kawamoto²,

Uemori³

et al. 2017

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Abstract. Supercritical carbon dioxide (SCCO2) is one of the green methods to extract plant matters substances. In this study, SCCO2 has been applied as a media to extract the grains of paradise seeds substances at temperatures of 40 -80 o C and pressures of 20 -40 MPa using a semi-continuous flow-type system. Results of FT-IR spectra analysis showed that the grains of paradise seeds substances were extracted by SCCO2. The SEM images of the grains of paradise seeds residues also showed that the physical changes of the grains of paradise seeds surface occurred after SCCO2 extraction. The main compounds in extracts were gingerols, shogaols, and paradols. However, the GC-MS may only identify paradol compounds due to gingerols, shogaols, and paradols are homologous series of phenolic ketones and paradols was more stable than gingerols and shogaols. The yields of gingerols, shogaols, and paradols were 9.12, 3.90, and 4.42 mg/g sample, respectively and affected by extraction temperatures and/or extraction pressures.

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“…It is very commonly found in both fresh water and saline water. C. vulgaris has high photosynthetic efficiency as well as high biomass productivity and is strongly resistant against harsh conditions and invaders, which make it ideal as a source of proteins, enzyme, lipids, carbohydrates, pigments, vitamins and minerals [3][4][5][6][7]. To break the hard cell wall and release the internal components of C. vulgaris, multiple cell disruption techniques have been employed, the most frequently used techniques include high pressure homogenization, autoclaving, enzymatic lysis, bead milling, grinding, etc.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Cell Disruption of Chlorella Vulgaris by Pressure-Assisted Ozonation and Ultrasonication

et al. 2016

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This study evaluated the effectiveness of pressure-assisted ozonation (PAO) in Chlorella vulgaris (C. vulgaris) cell disruption, and compared the disruption result with that of the ultrasonication (US) by using four quantification indicators: cell counting, ultra violet (UV) absorbance, turbidity and visible light absorbance. It was found that under the condition of 0.8 MPa and 80 cycles, PAO treatment achieved cell rupture of 80.3%, with the power of 1080 W and treatment time of 60 min, US achieved cell rupture of 83.8%. Cell counting was a reliable indicator and applicable to both PAO and US treatments. Turbidity and visible light absorbance gave similar results and featured as the simplest operation. UV absorbance reflected the metabolite release due to cell breakage; however, it was less reproducible when it was applied to quantify the cell rupture by PAO. Its trend indicated that during cell disruption metabolite degradation occurred, especially after significant rupture in the case of excessive PAO treatment. The cellular morphology of C. vulgaris cells during PAO and US treatments was investigated by scanning electron microscope (SEM) which certified that the cells damage was caused by both physical and chemical attack.

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Supercritical CO₂ extraction of pigment components with pharmaceutical importance from Chlorella vulgaris

Cited by 104 publications

References 19 publications

Economical Wet Extraction of Lipid from labyrinthula Aurantiochytrium limacinum by Using Liquefied Dimethyl Ether

Economical Wet Extraction of Lipid from labyrinthula Aurantiochytrium limacinum by Using Liquefied Dimethyl Ether

Extraction of Phytochemicals from Grains of Paradise Using Supercritical Carbon Dioxide

Evaluation of Cell Disruption of Chlorella Vulgaris by Pressure-Assisted Ozonation and Ultrasonication

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Supercritical CO2 extraction of pigment components with pharmaceutical importance from Chlorella vulgaris

Cited by 104 publications

References 19 publications

Economical Wet Extraction of Lipid from labyrinthula Aurantiochytrium limacinum by Using Liquefied Dimethyl Ether

Economical Wet Extraction of Lipid from labyrinthula Aurantiochytrium limacinum by Using Liquefied Dimethyl Ether

Extraction of Phytochemicals from Grains of Paradise Using Supercritical Carbon Dioxide

Evaluation of Cell Disruption of Chlorella Vulgaris by Pressure-Assisted Ozonation and Ultrasonication

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Supercritical CO₂ extraction of pigment components with pharmaceutical importance from Chlorella vulgaris