Supercritical carbon dioxide extraction of lipids from Pythium irregulare

Abstract

Lipids that contain polyunsaturated fatty acids (PUFA) have therapeutic value. PUFA, however, degrade in high-temperature, oxygen-rich conditions typical of conventional hot solvent-extraction and distillation methods. Supercritical CO 2 extraction was chosen as an alternative method to recover these valuable compounds from the lower fungus, Pythium irregulare. Freeze-dried biomass was subjected to an aqueous phase and placed into a flow-through extraction apparatus. Extraction of oil from this biomass showed some success for moisture contents as high as 30% (wet basis). The addition of a novel CO 2 -philic surfactant to the wet biomass with moisture contents as high as 95% (wet basis) increased the extraction rate of fungal oil by more than an order of magnitude. For tests with extraction times of 5 to 6 h, data for the diffusioncontrolled region were modeled with an analytical solution to Fick's second law. Equilibrium data were also obtained for the fungal oil at two isotherms (40 and 60°C) over a pressure range of 13.7 to 27.5 MPa.Paper no. J8977 in JAOCS 76, 595-602 (May 1999).

Enzyme-assisted extraction of lipids directly from the culture of the oleaginous yeast Rhodosporidium toruloides

“…After heating to 95°C for 30 min, the sample was immediately mixed with n-hexane for 5 min, and the LEY was 77% (Balasubramanian et al, 2011). The SCF method has also been applied to lipid extraction from the wet cell mass, but was not applicable to high moisture content feed (Walker et al, 1999). Mixing of high moisture cell mass with a scCO 2 -soluble surfactant enabled better extraction of the lipids.…”

Application of supercritical CO2 in lipid extraction – A review

“…It was found that supercritical CO 2 extraction at 30 MPa and 50°C yielded greater than 110% of the c-linolenic acid that was obtained by chemical extraction. Walker et al (1999) also extracted x-3 fatty acids using SFE from P.…”

Techniques conventionelles et innovantes, et solvants alternatifs pour l’extraction des lipides de micro-organismes

“…L'extraction peut également être optimisée par une intensification des conditions de température et de pression permettant ainsi d'augmenter le pouvoir de solvatation du solvant mis en jeu (Walker et al, 1999 ;Chen et al, 2011).…”