Supercritical angle fluorescence (SAF) microscopy

Ruckstuhl, Thomas; Verdes, Dorinel

doi:10.1364/opex.12.004246

Cited by 106 publications

(96 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The optical set-up is inverse to that of TIRF with an excitation beam illuminating the sample perpendicularly and a collection of fluorescence light emitted into the supercritical angle of the substrate using a parabolic mirror objective. The SAF technology itself (2000) [238,239], a SAF sensing instrument (2003) [240], and also a SAF imaging instrument (2007) [241,242] were recently developed. The strength of the SAF technique to study protein adsorption phenomena was demonstrated in some recent publications [44,102,118,187] or TIRF set-ups and, more recently, also with the SAF optics [244].…”

Section: Fluorescence Detection Techniquesmentioning

confidence: 99%

Understanding protein adsorption phenomena at solid surfaces

Rabe

Verdes

Seeger

2011

Advances in Colloid and Interface Science

Self Cite

1,345

1,239

View full text Add to dashboard Cite

Protein adsorption at solid surfaces plays a key role in many natural processes and has therefore promoted a widespread interest in many research areas. Despite considerable progress in this field there are still widely differing and even contradictive opinions on how to explain the frequently observed phenomena such as structural rearrangements, cooperative adsorption, overshooting adsorption kinetics, or protein aggregation. In this review recent achievements and new perspectives on protein adsorption processes are comprehensively discussed. The main focus is put on commonly postulated mechanistic aspects and their translation into mathematical concepts and model descriptions. Relevant experimental and computational strategies to practically approach the field of protein adsorption mechanisms and their impact on current successes are outlined.

show abstract

Section: Fluorescence Detection Techniquesmentioning

confidence: 99%

Understanding protein adsorption phenomena at solid surfaces

Rabe

Verdes

Seeger

2011

Advances in Colloid and Interface Science

Self Cite

1,345

1,239

View full text Add to dashboard Cite

show abstract

“…This is commonly achieved by an evanescent illumination of the sample by TIR of the excitation light at the cover slip-sample interface [15]. TIR fluorescence microscopy (TIRFM) is receiving much attention, both because of its simple implementation using either lenses of high numerical aperture or prism-based illumination, and its exceptional performance with respect to the contrast of the imaged structures at the interface versus structures in the volume (see for example [16][17][18][19][20][21][22][23]). TIRFM only detects the fluorophores near the cover glass-sample interface because of the finite penetration depth of the evanescent excitation field of typically <100 nm.…”

Section: Introductionmentioning

confidence: 99%

Fluorescence correlation spectroscopy with a total internal reflection fluorescence STED microscope (TIRF-STED-FCS)

Leutenegger¹,

Ringemann²,

Lasser³

et al. 2012

Opt. Express

View full text Add to dashboard Cite

We characterize a novel fluorescence microscope which combines the high spatial discrimination of a total internal reflection epi-fluorescence (epi-TIRF) microscope with that of stimulated emission depletion (STED) nanoscopy. This combination of high axial confinement and dynamic-active lateral spatial discrimination of the detected fluorescence emission promises imaging and spectroscopy of the structure and function of cell membranes at the macro-molecular scale. Following a full theoretical description of the sampling volume and the recording of images of fluorescent beads, we exemplify the performance and limitations of the TIRF-STED nanoscope with particular attention to the polarization state of the laser excitation light. We demonstrate fluorescence correlation spectroscopy (FCS) with the TIRF-STED nanoscope by observing the diffusion of dye molecules in aqueous solutions and of fluorescent lipid analogs in supported lipid bilayers in the presence of background signal. The nanoscope reduced the out-of-focus background signal. A lateral resolution down to 40-50 nm was attained which was ultimately limited by the low lateral signal-tobackground ratio inherent to the confocal epi-TIRF scheme. Together with the estimated axial confinement of about 55 nm, our TIRF-STED nanoscope achieved an almost isotropic and less than 1 attoliter small all-optically induced measurement volume.

show abstract

“…at the interface. Introduced either with a scanning approach [4] or with full-field imaging [5,6] the same axial resolution as TIRF can be obtained. Although fluorescence is a useful tool for biological study, this technique suffers from limitations.…”

Section: Biophotonicsmentioning

confidence: 99%

Label‐free evanescent microscopy for membrane nano‐tomography in living cells

Bon

Barroca

Lévêque‐Fort

et al. 2013

Journal of Biophotonics

View full text Add to dashboard Cite

We show that through‐the‐objective evanescent microscopy (epi‐EM) is a powerful technique to image membranes in living cells. Readily implementable on a standard inverted microscope, this technique enables full‐field and real‐time tracking of membrane processes without labeling and thus signal fading. In addition, we demonstrate that the membrane/interface distance can be retrieved with 10 nm precision using a multilayer Fresnel model. We apply this nano‐axial tomography of living cell membranes to retrieve quantitative information on membrane invagination dynamics. (© 2014 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)

show abstract

Supercritical angle fluorescence (SAF) microscopy

Cited by 106 publications

References 0 publications

Understanding protein adsorption phenomena at solid surfaces

Understanding protein adsorption phenomena at solid surfaces

Fluorescence correlation spectroscopy with a total internal reflection fluorescence STED microscope (TIRF-STED-FCS)

Label‐free evanescent microscopy for membrane nano‐tomography in living cells

Contact Info

Product

Resources

About