Supercomplex formation between Mlh1–Mlh3 and Sgs1–Top3 heterocomplexes in meiotic yeast cells

Wang, Ting-Fang; Kung, Wen-Mei

doi:10.1016/s0006-291x(02)02034-x

Cited by 36 publications

(33 citation statements)

References 32 publications

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“…Furthermore, Sgs1 and its helicase activity is part of the crossover-specific pathway together with Mlh1-Mlh3 3 (29,32). As Sgs1 shows a preference for unwinding HJs and it interacts with Mlh3 during meiosis (37,62), it is possible also that the Sgs1 helicase may act in complex with Mlh1-Mlh3 to melt the HJ structure. Finally, Exo1 has a non-catalytic role in promoting joint molecule resolution by Mlh1-Mlh3.…”

Section: Discussionmentioning

confidence: 99%

The Saccharomyces cerevisiae Mlh1-Mlh3 Heterodimer Is an Endonuclease That Preferentially Binds to Holliday Junctions

Ranjha

Anand

Ćejka

2014

Journal of Biological Chemistry

116

185

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Background: Mlh1-Mlh3 is required for meiotic interference-dependent crossovers. Results: We produced recombinant Mlh1-Mlh3 and show that it is an endonuclease that binds specifically Holliday junctions. Conclusion: Mlh1-Mlh3 prefers to bind the open conformation of Holliday junctions, which infers that it acts as part of a larger complex to process Holliday junctions in meiosis. Significance: Recombinant Mlh1-Mlh3 complexes will be invaluable for further studies.

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Section: Discussionmentioning

confidence: 99%

The Saccharomyces cerevisiae Mlh1-Mlh3 Heterodimer Is an Endonuclease That Preferentially Binds to Holliday Junctions

Ranjha

Anand

Ćejka

2014

Journal of Biological Chemistry

116

185

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“…It was, therefore, suggested that Mlh3 could either exist alone or interact with a different partner (11). Immunoprecipitation experiments revealed a direct interaction of scMlh3p with Sgs1 helicase in meiotic S. cerevisiae cells (34) and hMLH3 was shown to bind hMSH4 in meiotic human cells (14); however, the identification of the putative hMLH3 partners that might help stabilize it in mitotic cells must await the results of future experiments.…”

Section: Discussionmentioning

confidence: 99%

Expression of the MutL Homologue hMLH3 in Human Cells and its Role in DNA Mismatch Repair

et al. 2005

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The human mismatch repair (MMR) proteins hMLH1 and hPMS2 function in MMR as a heterodimer. Cells lacking either protein have a strong mutator phenotype and display microsatellite instability, yet mutations in the hMLH1 gene account for f50% of hereditary nonpolyposis colon cancer families, whereas hPMS2 mutations are substantially less frequent and less penetrant. Similarly, in the mouse model, Mlh1

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“…All strains are derivatives of SK1 (30). Yeast culture and sporulation techniques have been described (31,32). All deletion mutant strains and the Mnd1-His-6 strain were constructed by a PCR-mediated method using a KanMX6 module (33).…”

Section: Methodsmentioning

confidence: 99%

Heterodimeric complexes of Hop2 and Mnd1 function with Dmc1 to promote meiotic homolog juxtaposition and strand assimilation

Chen

Leng

Olivares

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

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108

164

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Saccharomyces cerevisiae Hop2 and Mnd1 are abundant meiosisspecific chromosomal proteins, and mutations in the corresponding genes lead to defects in meiotic recombination and in homologous chromosome interactions during mid-prophase. Analysis of various double mutants suggests that HOP2, MND1, and DMC1 act in the same genetic pathway for the establishment of close juxtaposition between homologous meiotic chromosomes. Biochemical studies indicate that Hop2 and Mnd1 proteins form a stable heterodimer with a higher affinity for double-stranded than single-stranded DNA, and that this heterodimer stimulates the strand assimilation activity of Dmc1 in vitro. Together, the genetic and biochemical results suggest that Hop2, Mnd1, and Dmc1 are functionally interdependent during meiotic DNA recombination.

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Supercomplex formation between Mlh1–Mlh3 and Sgs1–Top3 heterocomplexes in meiotic yeast cells

Cited by 36 publications

References 32 publications

The Saccharomyces cerevisiae Mlh1-Mlh3 Heterodimer Is an Endonuclease That Preferentially Binds to Holliday Junctions

The Saccharomyces cerevisiae Mlh1-Mlh3 Heterodimer Is an Endonuclease That Preferentially Binds to Holliday Junctions

Expression of the MutL Homologue hMLH3 in Human Cells and its Role in DNA Mismatch Repair

Heterodimeric complexes of Hop2 and Mnd1 function with Dmc1 to promote meiotic homolog juxtaposition and strand assimilation

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