Supercharged MPNs? Automated Determination of High-Throughput Most Probable Number (htMPN) Using Chip-Based 3D Digital PCR

Abstract

htMPN uses chip-based fluorescence acquisition and is a simple and compact tool for automatic viable cell enumeration with applications in microbiological research. This method applies to a wide range of anaerobic or facultative anaerobic species and improves accuracy by reducing the number of pipetting steps.

Progress in methods for the detection of viable Escherichia coli

“…To address this issue, Wang et al introduced a new approach for cell counting, called high-throughput MPN. 274 This method utilized a chip-based digital PCR instrument to rapidly and accurately quantify viable or suboptimal cells. Individual cells were cultured in individual wells of the chip at their optimal temperature until the cell density was sufficient to detect a fluorescent signal using a cell-permeable or cell-permeable DNA sandwich fluorescent dye.…”