Abstract:htMPN uses chip-based fluorescence acquisition and is a simple and compact tool for automatic viable cell enumeration with applications in microbiological research. This method applies to a wide range of anaerobic or facultative anaerobic species and improves accuracy by reducing the number of pipetting steps.
“…To address this issue, Wang et al introduced a new approach for cell counting, called high-throughput MPN. 274 This method utilized a chip-based digital PCR instrument to rapidly and accurately quantify viable or suboptimal cells. Individual cells were cultured in individual wells of the chip at their optimal temperature until the cell density was sufficient to detect a fluorescent signal using a cell-permeable or cell-permeable DNA sandwich fluorescent dye.…”
Escherichia coli (E. coli) is a prevalent enteric bacterium and a necessary organism to monitor for food safety and environmental purposes. Developing efficient and specific methods is critical for detecting...
“…To address this issue, Wang et al introduced a new approach for cell counting, called high-throughput MPN. 274 This method utilized a chip-based digital PCR instrument to rapidly and accurately quantify viable or suboptimal cells. Individual cells were cultured in individual wells of the chip at their optimal temperature until the cell density was sufficient to detect a fluorescent signal using a cell-permeable or cell-permeable DNA sandwich fluorescent dye.…”
Escherichia coli (E. coli) is a prevalent enteric bacterium and a necessary organism to monitor for food safety and environmental purposes. Developing efficient and specific methods is critical for detecting...
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.