SUPER STARCHY1/ONAC025 participates in rice grain filling

Mathew, Iny Elizebeth; Priyadarshini, Richa; Mahto, Arunima; Jaiswal, Praveen Kumar; Parida, Swarup K.; Agarwal, Pinky

doi:10.1002/pld3.249

Cited by 16 publications

(19 citation statements)

References 143 publications

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“…The same analysis has also been successfully done for rice transgenic plants with altered expression of another seed-specific gene ONAC025 . DEGs common to both transgenic plant and seed are the ones downstream to the transgene (Mathew et al, 2020 ). This analysis unveiled a correlation of DEGs in the OsGW2 _RNAi_7AP9 transgenic line with seed-related genes.…”

Section: Resultsmentioning

confidence: 99%

“…The confirmed clone was introduced into the Agrobacterium tumefaciens strain EHA105 . Transgenic plants were generated by transformation of the RNAi construct into rice calli (PB1 genotype IET-10364) through the Agrobacterium -mediated transformation method (Toki et al, 2006 ; Das et al, 2019 ; Mathew et al, 2020 ).…”

Section: Methodsmentioning

confidence: 99%

“…Transcriptome sequencing was performed on Illumina Hiseq TM 2000 platform with two independent biological replicates, each for WT and OsGW2 _RNAi_7AP9. After obtaining sequencing data, different tools were used for performing preprocessing of data, alignment with reference, expression estimation, and comparison analysis, as detailed previously (Mathew et al, 2020 ). The preprocessing tools used were Adapter Removal v2 (version 2.2.0) and bowtie2 (version 2.2.9).…”

Section: Methodsmentioning

confidence: 99%

“…Functional annotation of DEGs in metabolic pathways/processes was studied by using databases including the KEGG pathway analysis and MSU-RGAP 7 (Ouyang et al, 2006 ). Characterized differentially expressed genes were separated and analyzed using RICENCODE, and seed-related genes were extracted by comparing DEGs with the data available from previous studies in the lab and online sources, as done previously (Mathew et al, 2020 ). Heat maps were constructed using MeV Version 4.9.0 (Saeed et al, 2003 ).…”

Section: Methodsmentioning

confidence: 99%

“…RNA Isolation, cDNA Preparation, and Quantitative RT-PCR Isolation of RNA from the leaves and seeds (S1-S5 stages) of different rice genotypes (PB1, IR64, Sonasal, LGR, and Nipponbare) as well as transgenic plants was carried out using TRIzol R reagent (Invitrogen TM Life Technologies, USA) as detailed previously (Singh et al, 2003;Mathew et al, 2016Mathew et al, , 2020Das et al, 2019). The quantification of RNA samples was done by NANODROP 2000c Spectrophotometer (Thermo Scientific, USA).…”

Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%

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Silencing of an Ubiquitin Ligase Increases Grain Width and Weight in indica Rice

et al. 2021

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Many quantitative trait loci (QTLs) have been identified by molecular genetic studies which control grain size by regulating grain width, length, and/or thickness. Grain width 2 (GW2) is one such QTL that codes for a RING-type E3 ubiquitin ligase and increases grain size by regulating grain width through ubiquitin-mediated degradation of unknown substrates. A natural variation (single-nucleotide polymorphism at the 346th position) in the functional domain-coding region of OsGW2 in japonica rice genotypes has been shown to cause an increase in grain width/weight in rice. However, this variation is absent in indica rice genotypes. In this study, we report that reduced expression of OsGW2 can alter grain size, even though natural sequence variation is not responsible for increased grain size in indica rice genotypes. OsGW2 shows high expression in seed development stages and the protein localizes to the nucleus and cytoplasm. Downregulation of OsGW2 by RNAi technology results in wider and heavier grains. Microscopic observation of grain morphology suggests that OsGW2 determines grain size by influencing both cell expansion and cell proliferation in spikelet hull. Using transcriptome analysis, upregulated genes related to grain size regulation have been identified among 1,426 differentially expressed genes in an OsGW2_RNAi transgenic line. These results reveal that OsGW2 is a negative regulator of grain size in indica rice and affects both cell number and cell size in spikelet hull.

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Section: Resultsmentioning

confidence: 99%