Super-resolution fluorescence polarization microscopy

Zhanghao, Karl; Gao, Juntao; Jin, Dayong; Zhang, Xuedian; Xi, Peng

doi:10.1142/s1793545817300026

Cited by 23 publications

(15 citation statements)

References 70 publications

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“… a Stokes vector based approaches: (i) DOP, DOLP and DOCP 2 – 5 ; (ii) Stokes vector projection approach 158 ; (iii) Stokes vector location approach 159 ; (iv) Dipole orientation differentiation approach. Polarisation angle (PA) and intensity are used as main parameters 154 , 155 . b MM based approaches: (i) MMPD method 52 : diattenuation (D), retardance (R) and depolarisation (Δ) (all of them maintain linear/circular components); and (ii) MMT method 57 : depolarisation (1-b; associated with small molecule scattering), level of linear anisotropy (t1), azimuth orientation of the anisotropy (α1), and more 179 ; (iii) PFP method 95 ; (iv) Property of symmetry and asymmetry 168 , 169 .…”

Section: Vectorial Information Extraction Methods For Biomedical Applicationsmentioning

confidence: 99%

Polarisation optics for biomedical and clinical applications: a review

et al. 2021

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Many polarisation techniques have been harnessed for decades in biological and clinical research, each based upon measurement of the vectorial properties of light or the vectorial transformations imposed on light by objects. Various advanced vector measurement/sensing techniques, physical interpretation methods, and approaches to analyse biomedically relevant information have been developed and harnessed. In this review, we focus mainly on summarising methodologies and applications related to tissue polarimetry, with an emphasis on the adoption of the Stokes–Mueller formalism. Several recent breakthroughs, development trends, and potential multimodal uses in conjunction with other techniques are also presented. The primary goal of the review is to give the reader a general overview in the use of vectorial information that can be obtained by polarisation optics for applications in biomedical and clinical research.

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Section: Vectorial Information Extraction Methods For Biomedical Applicationsmentioning

confidence: 99%

Polarisation optics for biomedical and clinical applications: a review

et al. 2021

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“…The electromagnetic radiation from a molecular chromophore is proportional to sine-squared (sin 2 ) of the intersecting angle between emission dipole orientation and direction of observation. Mostly the absorption dipole moment orientation and emission dipole moment orientation of fluorophores can be regarded as parallel [4] , [24] , [25] .…”

Section: Principles Of Fpmmentioning

confidence: 99%

Advances of super-resolution fluorescence polarization microscopy and its applications in life sciences

Chen

Yang

et al. 2020

Computational and Structural Biotechnology Journal

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Fluorescence polarization microscopy (FPM) analyzes both intensity and orientation of fluorescence dipole, and reflects the structural specificity of target molecules. It has become an important tool for studying protein organization, orientational order, and structural changes in cells. However, suffering from optical diffraction limit, conventional FPM has low orientation resolution and observation accuracy, as the polarization information is averaged by multiple fluorescent molecules within a diffraction-limited volume. Recently, novel super-resolution FPMs have been developed to break the diffraction barrier. In this review, we will introduce the recent progress to achieve sub-diffraction determination of dipole orientation. Biological applications, based on polarization analysis of fluorescence dipole, are also summarized, with focus on chromophore-target molecule interaction and molecular organization.

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“…Nevertheless, an enhancement technique has been demonstrated on a monitor where the screen is physically moved in a circular path by employing a spinning weight [4]. Super-resolution in microscopy is described in [5]. In [6], a projector that uses native resolution light modulator panels to enhance projection resolution at selected regions is presented, which is achieved by decomposing a target high resolution image into a sparse edge image and a complementary lower resolution non-edge image, and then projecting these images in a time sequential manner at a high frame rate.…”

Section: Introductionmentioning

confidence: 99%

A Resolution-Enhanced Digital Micromirror Device (DMD) Projection System

Zhang

Surman

2021

IEEE Access

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In this paper we present a resolution-enhanced system for digital micromirror device (DMD) projected images by utilizing time multiplexing and birefringence in order to achieve this with static components only. This provides a less expensive solution than reducing the size of the hardware pixels and can also be applied to higher resolution displays, as and when, these become available. Birefringent materials have different refractive indices for linearly polarized light with orthogonal polarization directions. A highresolution projected image can be observed by overlapping two or three native resolution images at shifts of one half or one third of a DMD pixel diagonal respectively on successive frames. The optical components comprise a custom-made quartz plate as the birefringent component and an off-the-shelf twisted nematic liquid crystal display (TN-LCD) screen as a polarization rotator that is synchronized with the DMD frame rate. This paper covers the principle of operation and the system design. The functioning of the display is verified using enlarged images of the letter 'R' and a checkerboard pattern that are formed from a small number of DMD pixels.

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Super-resolution fluorescence polarization microscopy

Cited by 23 publications

References 70 publications

Polarisation optics for biomedical and clinical applications: a review

Polarisation optics for biomedical and clinical applications: a review

Advances of super-resolution fluorescence polarization microscopy and its applications in life sciences

A Resolution-Enhanced Digital Micromirror Device (DMD) Projection System

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