Super-paramagnetic iron oxide nanoparticles for use in extrapulmonary tuberculosis diagnosis

Lee, C. N.; Wang, Y. M.; Lai, Wen-FuThomas; Chen, T. J.; Yu, Meijuan; Fang, Chia Lang; Yu, Fu-gong; Tsai, Yu‐Hui; Chang, Walter; Chen, Zuo; Renshaw, P. F.

doi:10.1111/j.1469-0691.2012.03809.x

Cited by 33 publications

(12 citation statements)

References 41 publications

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“…Cell labeling and tracking experiments are commonly performed either by labeling immune cells in vitro and regrafting them or by systemic application of iron oxide particles which are then phagocytosed by macrophages [21]. By using negative iron contrast immunological reactions and bacteria-induced inflammatory dissemination have already been investigated in animal models of Staphylococcus aureus [22-26], Toxoplasma gondii [27], Mycobacterium tuberculosis [28] or LPS-induced sepsis conditions [29,30]. However, only BLI so far was capable of visualizing bacteria directly.…”

Section: Introductionmentioning

confidence: 99%

Bacteria tracking by in vivomagnetic resonance imaging

et al. 2013

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BackgroundDifferent non-invasive real-time imaging techniques have been developed over the last decades to study bacterial pathogenic mechanisms in mouse models by following infections over a time course. In vivo investigations of bacterial infections previously relied mostly on bioluminescence imaging (BLI), which is able to localize metabolically active bacteria, but provides no data on the status of the involved organs in the infected host organism. In this study we established an in vivo imaging platform by magnetic resonance imaging (MRI) for tracking bacteria in mouse models of infection to study infection biology of clinically relevant bacteria.ResultsWe have developed a method to label Gram-positive and Gram-negative bacteria with iron oxide nano particles and detected and pursued these with MRI. The key step for successful labeling was to manipulate the bacterial surface charge by producing electro-competent cells enabling charge interactions between the iron particles and the cell wall. Different particle sizes and coatings were tested for their ability to attach to the cell wall and possible labeling mechanisms were elaborated by comparing Gram-positive and -negative bacterial characteristics. With 5-nm citrate-coated particles an iron load of 0.015 ± 0.002 pg Fe/bacterial cell was achieved for Staphylococcus aureus. In both a subcutaneous and a systemic infection model induced by iron-labeled S. aureus bacteria, high resolution MR images allowed for bacterial tracking and provided information on the morphology of organs and the inflammatory response.ConclusionLabeled with iron oxide particles, in vivo detection of small S. aureus colonies in infection models is feasible by MRI and provides a versatile tool to follow bacterial infections in vivo. The established cell labeling strategy can easily be transferred to other bacterial species and thus provides a conceptual advance in the field of molecular MRI.

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Section: Introductionmentioning

confidence: 99%

Bacteria tracking by in vivomagnetic resonance imaging

et al. 2013

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“…10 Metal-based NPs, for example, have an important application as nano-sized sensors for biological labeling and as vectors for drug delivery. 11,12 However, many metal NPs, such as zinc oxide NPs, have adverse effects on human health because of their high bioreactivity.…”

Section: Introductionmentioning

confidence: 99%

Allergenicity and toxicology of inhaled silver nanoparticles in allergen-provocation mice models

Chuang¹,

Hsiao²,

Wu³

et al. 2013

IJN

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Silver nanoparticles (AgNP) have been associated with the exacerbation of airway hyperresponsiveness. However, the allergenicity and toxicology of AgNP in healthy and allergic individuals are unclear. We investigated the pathophysiological responses to AgNP inhalation in a murine model of asthma. Continuous and stable levels of 33 nm AgNP were maintained at 3.3 mg/m 3 during the experimental period. AgNP exposure concomitant with ovalbumin challenge increased the enhanced pause (Penh) in the control and allergic groups. AgNP evoked neutrophil, lymphocyte and eosinophil infiltration into the airways and elevated the levels of allergic markers (immunoglobulin E [IgE] and leukotriene E 4 [LTE 4 ]), the type 2 T helper (Th2) cytokine interleukin-13 (IL-13), and oxidative stress (8-hydroxy-2′-deoxyguanosine [8-OHdG]) in healthy and allergic mice. Bronchocentric interstitial inflammation was observed after AgNP inhalation. After inhalation, the AgNP accumulated predominantly in the lungs, and trivial amounts of AgNP were excreted in the urine and feces. Furthermore, the AgNP induced inflammatory responses in the peritoneum. The inhalation of AgNP may present safety concerns in healthy and susceptible individuals.

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“…Here the nanoparticles are conjugated to anti-tuberculosis antibodies and administered to the subject. The antibodies bind to the bacteria and the nanoparticles act as a contrast agent in an MRI scan, allowing the infected areas to be imaged 39. In cancer diagnosis nanoparticles are acting as contrast agents and have been used in studies of pancreatic cancer, again being conjugated to antibodies specific to cancer cell markers 40.…”

Section: Nanoparticlesmentioning

confidence: 99%

Nanoparticles Based Stem Cell Tracking in Regenerative Medicine

Edmundson¹,

Thanh²,

Song³

2013

Theranostics

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Stem cell therapies offer great potentials in the treatment for a wide range of diseases and conditions. With so many stem cell replacement therapies going through clinical trials currently, there is a great need to understand the mechanisms behind a successful therapy, and one of the critical points of discovering them is to track stem cell migration, proliferation and differentiation in vivo. To be of most use tracking methods should ideally be non-invasive, high resolution and allow tracking in three dimensions. Magnetic resonance imaging (MRI) is one of the ideal methods, but requires a suitable contrast agent to be loaded to the cells to be tracked, and one of the most wide-spread in stem cell tracking is a group of agents known as magnetic nanoparticles. This review will explore the current use of magnetic nanoparticles in developing and performing stem cell therapies, and will investigate their potential limitations and the future directions magnetic nanoparticle tracking is heading in.

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Super-paramagnetic iron oxide nanoparticles for use in extrapulmonary tuberculosis diagnosis

Cited by 33 publications

References 41 publications

Bacteria tracking by in vivomagnetic resonance imaging

Bacteria tracking by in vivomagnetic resonance imaging

Allergenicity and toxicology of inhaled silver nanoparticles in allergen-provocation mice models

Nanoparticles Based Stem Cell Tracking in Regenerative Medicine

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