Super‐marathon race increases serum and urinary nitrotyrosine and carbonyl levels

Radák, Zsolt; Ogonovszky, Helga; Dubecz, J.; Pavlik, G.; Sasvári, Mária; Pucsok, József Márton; Berkes, István; Csont, Tamás; Ferdinándy, Péter

doi:10.1046/j.1365-2362.2003.01202.x

Cited by 56 publications

(46 citation statements)

References 28 publications

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“…Our results did not show basal MDA and AOPP changes after maximal exercise in normoxic conditions despite the number of studies reporting a rise of markers lipid oxidation (Sen, 1995) or protein oxidation (Radak et al, 2003) in the plasma after strenuous exercise. This discrepancy may be due to the higher training level of our subjects who might improve their pro-oxidant/antioxidant balance.…”

Section: Effects Of Maximal Exercise Under Normobaric Hypoxia and Norcontrasting

confidence: 99%

Effects of exercise and training in hypoxia on antioxidant/pro-oxidant balance

Pialoux¹,

Mounier²,

Ponsot

et al. 2006

Eur J Clin Nutr

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Objective: The aim was to investigate the effects of acute exercise under hypoxic condition and the repetition of such exercise in a 'living low-training high' training on the antioxidant/prooxidant balance. Design: Randomized, repeated measures design. Setting: Faculté de Médecine, Clermont-Ferrand, France. Subjects: Fourteen runners were randomly divided into two groups. A 6-week endurance training protocol integrated two running sessions per week at the second ventilatory threshold into the usual training. Intervention: A 6-week endurance training protocol integrated two running sessions per week at the second ventilatory threshold into the usual training. The first hypoxic group (HG, n ¼ 8) carried out these sessions under hypoxia (3000 m simulated altitude) and the second normoxic group (NG, n ¼ 6) in normoxia. In control period, the runners were submitted to two incremental cycling tests performed in normoxia and under hypoxia (simulated altitude of 3000 m). Plasma levels of advanced oxidation protein products (AOPP), malondialdehydes (MDA) and lipid oxidizability, ferric-reducing antioxidant power (FRAP), lipid-soluble antioxidants (a-tocopherol and b-carotene) normalized for triacyglycerols and cholesterol were measured before and after the two incremental tests and at rest before and after training. Results: No significant changes of MDA and AOPP level were observed after normoxic exercise, whereas hypoxic exercise induced a 56% rise of MDA and a 44% rise of AOPP. Plasma level of MDA and arterial oxygen hemoglobin desaturations after the acute both exercises were highly correlated (r ¼ 0.73). a-Tocopherol normalized for cholesterol and triacyglycerols increased only after hypoxic exercise (10-12%, Po0.01). After training, FRAP resting values (À21%, Po0.05) and a-tocopherol/ triacyglycerols ratio (À24%, Po0.05) were diminished for HG, whereas NG values remained unchanged. Conclusions: Intense exercise and hypoxia exposure may have a cumulative effect on oxidative stress. As a consequence, the repetition of such exercise characterizing the 'living low-training high' model has weakened the antioxidant capacities of the athletes. Sponsorship: International Olympic Committee and the Direction Régionale de la Jeunesse et des Sports de la Région Auvergne.

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Section: Effects Of Maximal Exercise Under Normobaric Hypoxia and Norcontrasting

confidence: 99%

Effects of exercise and training in hypoxia on antioxidant/pro-oxidant balance

Pialoux¹,

Mounier²,

Ponsot

et al. 2006

Eur J Clin Nutr

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“…An internal standard (American Type Culture Collection, Manassas, VA) was loaded into each gel to normalize activities between gels. After electrophoresis (150 V, 1.5 h), gels were washed with 2.5% Triton X-100 for 3 x 15 min and incubated for 20 h at 37°C in incubation buffer (50 mM Tris·HCl, 150 mM NaCl, 5 mM CaCl2, and 0.05% NaN3, To estimate systemic peroxynitrite formation, we have measured free nitrotyrosine by ELISA in serum samples as previously described [16,17]. Briefly, serum samples were deproteinized by addition of 4 volumes of ice-cold ethanol.…”

Section: Biochemical Analysesmentioning

confidence: 99%

Serum lipids and cardiac function correlate with nitrotyrosine and MMP activity in coronary artery disease patients

Bencsik

Sasi

Kiss

et al. 2015

Eur J Clin Investigation

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“…Os atletas em nosso estudo não necessariamente chegaram à exaustão, tinham experiência de treinamento maior que 12 semanas e provavelmente mantiveram-se no metabolismo aeróbio a maior parte da competição, o que poderia explicar a diferença entre os resultados encontrados. Ainda assim, Radák et al (25) observaram, após 93km de corrida, aumento nas carbonilas plasmáticas e que esse aumento estava correlacionado com as carbonilas urinárias, sugerindo um marcador não invasivo de EO.…”

Section: Discussionunclassified

Efeito do exercício de ultrarresistência sobre parâmetros de estresse oxidativo

Schneider

Silveira

Moreira

et al. 2009

Rev Bras Med Esporte

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Introdução: Exercícios de longa duração podem levar ao desequilíbrio entre os sistemas pró e antioxidante, acarretando dano a lipídeos, proteínas e DNA. Entretanto, alguns estudos avaliando triatlo Ironman observaram proteção aos lipídeos. Objetivo: Avaliar parâmetros de estresse oxidativo após uma competição de meio Ironman. Métodos: Participaram 11 sujeitos com idade de 31,1 ± 3,3 anos, massa corporal de 72,4 ± 5,4kg, estatura de 176,2 ± 4,8cm, gordura corporal de 9,8 ± 3,3 %, . VO 2máx na corrida de 60,7 ± 6,0mL/kg/min. Foram mensurados: dano a lipídeos através da quimiluminescência nos eritrócitos e TBARS no plasma, dano a proteínas através das carbonilas plasmáticas, ácido úrico e compostos fenólicos plasmáticos, assim como a atividade antioxidante enzimática da catalase, superóxido dismutase e glutationa peroxidase nos eritrócitos. Resultados: Houve redução na atividade da enzima superóxido dismutase (23,24 ± 1,49 para 20,77 ± 2,69U SOD/mg proteína, p = 0,045), e aumento no ácido úrico (40,81 ± 10,68 para 60,33 ± 6,71mg/L, p < 0,001) logo após a competição. Não houve diferença estatisticamente significativa na atividade das enzimas antioxidantes catalase e glutationa peroxidase e nos compostos fenólicos totais, assim como não foi observado dano a lipídeos (TBARS e quimiluminescência) e proteínas (carbonilas). Conclusão: Esse grupo de atletas não sofreu estresse oxidativo, provavelmente devido à liberação de ácido úrico e outros antioxidantes no plasma.Palavras-chave: radicais livres, ácido úrico, superóxido dismutase. ABSTRACTUltra-endurance exercises can cause imbalance between the pro and antioxidant systems, leading to lipid, protein and DNA damage. Nevertheless, some studies evaluating Ironman triathlon found protection to lipids. Purpose: To evaluate oxidative stress parameters after a half-Ironman competition. Methods: Eleven subjects aged 31.1 ± 3.3 yr, body weight 72.4 ± 5.4 kg, height 176.2 ± 4.8 cm, body fat 9.8 ± 3.3 %, . VO 2máx on run 60.7 ± 6.0 mL/kg/min, participated in this study. The following data were measured: lipid damage by chemoluminescence in erythrocyte and TBARS in plasma, protein damage by plasmatic carbonyls, uric acid and phenolic compounds, as well as the antioxidant enzymatic activity of catalase, superoxide dismutase and glutathione peroxidase in erythrocytes. Results: Reduction in superoxide dismutase (23.24 ± 1.49 to 20.77 ± 2.69 U SOD/mg protein, p = 0.045), and increase in uric acid (40.81 ± 10.68 to 60.33 ± 6.71 mg/L, p < 0.001) were found immediately after the competition. No statistically significant differences were found in catalase and glutathione peroxidase or phenolic compounds. Lipid (TBARS and chemoluminescence) or protein (carbonyl) damage was not observed either. Conclusion: These athletes did not suffer oxidative stress, probably due to uric acid and other plasmatic antioxidants release in the plasma.Keywords: free radicals, uric acid, superoxide dismutase. INTRODUÇÃOExercícios extenuantes ou de longa duração estão relacionados ao dano tecidual e podem caus...

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Super‐marathon race increases serum and urinary nitrotyrosine and carbonyl levels

Cited by 56 publications

References 28 publications

Effects of exercise and training in hypoxia on antioxidant/pro-oxidant balance

Effects of exercise and training in hypoxia on antioxidant/pro-oxidant balance

Serum lipids and cardiac function correlate with nitrotyrosine and MMP activity in coronary artery disease patients

Efeito do exercício de ultrarresistência sobre parâmetros de estresse oxidativo

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