Super determinant1A, a RAWULdomain-containing protein, modulates axillary meristem formation and compound leaf development in tomato

López, Hernán Peredo; Schmitz, Gregor; Thoma, Rahere; Theres, Klaus

doi:10.1093/plcell/koab121

Cited by 9 publications

(8 citation statements)

References 70 publications

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“…Similarly, they also establish the repressive environment around WUS gene locus (Figure 1; Wang et al, 2017). By contrast, in tomato, PRC2 component Super Determinant 1A (Sde1A) and PRC1 component B cellspecific Moloney murine leukemia virus integration site 1 (Bmi1) collaborate synergistically in promoting but not suppressing AM formation, through impacting associated histone marks (Lopez et al, 2021). This finding also implies a complicated constitution and diverged roles of PRC1 and PRC2 complexes.…”

Section: Gene Regulatory Network Composed Of Transcription Factors and Epigenetic Regulatorsmentioning

confidence: 99%

Stem Cell Basis for Fractal Patterns: Axillary Meristem Initiation

Wang

2021

Front. Plant Sci.

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Whereas stem cell lineages are of enormous importance in animal development, their roles in plant development have only been appreciated in recent years. Several specialized lineages of stem cells have been identified in plants, such as meristemoid mother cells and vascular cambium, as well as those located in the apical meristems. The initiation of axillary meristems (AMs) has recently gained intensive attention. AMs derive from existing stem cell lineages that exit from SAMs and define new growth axes. AMs are in fact additional rounds of SAMs, and display the same expression patterns and functions as the embryonic SAM, creating a fractal branching pattern. Their formation takes place in leaf-meristem boundaries and mainly comprises two key stages. The first stage is the maintenance of the meristematic cell lineage in an undifferentiated state. The second stage is the activation, proliferation, and re-specification to form new stem cell niches in AMs, which become the new postembryonic “fountain of youth” for organogenesis. Both stages are tightly regulated by spatially and temporally interwound signaling networks. In this mini-review, I will summarize the most up-to-date understanding of AM establishment and mainly focus on how the leaf axil meristematic cell lineage is actively maintained and further activated to become CLV3-expressed stem cells, which involves phytohormonal cascades, transcriptional regulations, epigenetic modifications, as well as mechanical signals.

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Section: Gene Regulatory Network Composed Of Transcription Factors and Epigenetic Regulatorsmentioning

confidence: 99%

Stem Cell Basis for Fractal Patterns: Axillary Meristem Initiation

Wang

2021

Front. Plant Sci.

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“…A BLASTP search identified 138 homologs. Whereas Marchantia polymorpha and Selaginella moellendorffii contain a single gene, Physcomitrella patens , Amborella trichopoda , Pinus pinaster , and most dicots contain two genes, and monocots usually contain three ( López et al., 2021 ). The tomato, rice, and maize homologs are involved in shoot apical meristem and axillary meristem development ( Tabuchi et al., 2011 ; Yao et al., 2019 ; López et al., 2021 ).…”

Section: Introductionmentioning

confidence: 99%

“…Whereas Marchantia polymorpha and Selaginella moellendorffii contain a single gene, Physcomitrella patens , Amborella trichopoda , Pinus pinaster , and most dicots contain two genes, and monocots usually contain three ( López et al., 2021 ). The tomato, rice, and maize homologs are involved in shoot apical meristem and axillary meristem development ( Tabuchi et al., 2011 ; Yao et al., 2019 ; López et al., 2021 ). One of the tomato homologs, Super determinant1A (Sde1A), and the Marchatia polymorpha homolog, MpBMI1-L, have been shown to genetically interact with the BMI1 proteins ( Liu et al., 2021 ; López et al., 2021 ).…”

Section: Introductionmentioning

confidence: 99%

“…The tomato, rice, and maize homologs are involved in shoot apical meristem and axillary meristem development ( Tabuchi et al., 2011 ; Yao et al., 2019 ; López et al., 2021 ). One of the tomato homologs, Super determinant1A (Sde1A), and the Marchatia polymorpha homolog, MpBMI1-L, have been shown to genetically interact with the BMI1 proteins ( Liu et al., 2021 ; López et al., 2021 ). These findings raise the possibility that these genes may be involved in the regulation of PRC1 activity by, for instance, competing with BMI1 for interaction partners or, alternatively, in the formation of non-enzymatically active vPRC1s.…”

Section: Introductionmentioning

confidence: 99%

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Roles of Polycomb complexes in regulating gene expression and chromatin structure in plants

Baile

Gómez-Zambrano

Calonje

2022

Plant Communications

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The evolutionary conserved Polycomb Group (PcG) repressive system comprises two central protein complexes, PcG repressive complex 1 (PRC1) and PRC2. These complexes, through the incorporation of histone modifications on chromatin, have an essential role in the normal development of eukaryotes. In recent years, a significant effort has been made to characterize these complexes in the different kingdoms, and despite there being remarkable functional and mechanistic conservation, some key molecular principles have diverged. In this review, we discuss current views on the function of plant PcG complexes. We compare the composition of PcG complexes between animals and plants, highlight the role of recently identified plant PcG accessory proteins, and discuss newly revealed roles of known PcG partners. We also examine the mechanisms by which the repression is achieved and how these complexes are recruited to target genes. Finally, we consider the possible role of some plant PcG proteins in mediating local and long-range chromatin interactions and, thus, shaping chromatin 3D architecture.

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“…, SlySdeA1(Lopez et al, 2021), SlyHD8(Hua et al, 2021), SlyNOR(Gao et al, 2019), SlyTAGL1 (Liu et al, 2020), SlyLOL1 (Borovsky et al, 2019), SlyRIN (Li et al, 2020), SlyMLO1 (Nekrasov et al, 2017), SlyCPK28 (Hu et al, 2021), SlySp5G (Soyk et al, 2017), SlyRCM1 (Liu et al, 2021) and SlyPIF4 (Pan et al, 2021). Target site sequences were amplified from 10 T-DNA positive lines and analysed by Sanger sequencing.…”

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confidence: 99%

Guide RNA scaffold variants enabled easy cloning of large gRNA cluster for multiplexed gene editing

Wang,

Li,

Liu

et al. 2023

Plant Biotechnology Journal

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SummaryCas9 protein‐mediated gene editing has revolutionized genetic manipulation in most organisms. There are many cases where multiplexed gene editing is needed. Cas9 is capable of multiplex gene editing when expressed with multiple guide RNAs. Conventional cloning methods for multiplexed gene editing vector is not efficient due to repeated use of a single‐guide RNA scaffold and inefficient ligation. In this study, we conducted structure‐guided mutagenesis and random mutagenesis on the original sgRNA scaffold and identified a large number of functional sgRNA scaffold variants. With these scaffold variants and different tRNAs, fusion polymerase chain reaction protocol was developed to rapidly synthesize spacer‐scaffold‐tRNA‐spacer units with up to 9 targets. In conjunction with golden gate cloning, gene editing vectors with up to 24 target sites were efficiently cloned in one‐step cloning. One such gene editing vector targeting 12 genes in tomato were tested in stable transformation and 10 out of the 12 genes were found mutated in a single transgenic line. To facilitate the application of multiplexed gene editing using these scaffold variants and tRNAs from different species, a webserver was created to generate primer sets and provide template sequences for the synthesis of large sgRNA expression units based on the user‐supplied target sequences and species.

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Super determinant1A, a RAWULdomain-containing protein, modulates axillary meristem formation and compound leaf development in tomato

Cited by 9 publications

References 70 publications

Stem Cell Basis for Fractal Patterns: Axillary Meristem Initiation

Stem Cell Basis for Fractal Patterns: Axillary Meristem Initiation

Roles of Polycomb complexes in regulating gene expression and chromatin structure in plants

Guide RNA scaffold variants enabled easy cloning of large gRNA cluster for multiplexed gene editing

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