¹³C-Nuclear Magnetic Resonance Spectroscopic Studies on Saturated, Mono-, Di- and Polyunsaturated Fatty Acids, Phospho- and Sphingo- lipids

Nuclear Magnetic Relaxation and the Biological Membrane

“…All six glycerol and choline carbon nuclei are well resolved, and in CD 3 0D solutions, where the spectral lines are generally sharper, most show coupling to l4N and/or 3lp nuclei. Our assignments for these carbons, supported by selective proton-decoupling experiments, do not agree with the partial assignments subsequently suggested by Stoffel et al (1972). The chemical shifts of many of the l3C nuclei depend significantly on solvent: the observed variations are consistent with variations in local diamagnetic susceptibility ).…”

“…In the l3C spectrum of dioleoyl lecithin, additional resonances appear at low field due to the olefinic carbons, and new resonances separate from the methylene envelope assigned to carbon nuclei near the olefinic carbons (Levine et al, 1972a). These new resonances have been shown by specific 13C-Iabeling experiments to be due to carbons 8 and 11 (Stoffel et al, 1972).…”

Magic angle spinning 13C-NMR spin-lattice relaxation study of the location and effects of ?-tocopherol, ubiquinone-10 and ubiquinol-10 in unsonicated model membranes

“…Some more resonances, belonging to the terpenoid molecules, could also be observed. The assignment of the different resonances of the phospholipid was made according to Stoffel et al (1972) and Lee et al (1974). Because of the highly resolved nature of the MAS 13C-NMR spectra it was possible to determine the T1 of most individual carbon atom sites of EYL.…”

Phospholipids of rabbit and bull sperm membranes: Structural order parameter and steady‐state fluorescence anistropy of membranes and membrane leaflets

“…These molecules usually consist of highly unsaturated acyl chains that affect the structural configuration of phospholipids and also greatly reduce the condensing effect of cholesterol (Stubbs and Smith, 1984). The diacyl components differ in their properties (Stoffel and Heimann, 1973;Clegg, 1983). Alkenyl-acyl phospholipids form more rigid lipid structures (Drenthe et al, 1980;Smith, 1954).…”