In-plane microwave penetration depth λ ab and quaiparticle conductivity at 28 GHz are measured in underdoped single crystals of the Fe-based superconductor PrFeAsO1−y (Tc ≈ 35 K) by using a sensitive superconducting cavity resonator. λ ab (T ) shows flat dependence at low temperatures, which is incompatible with the presence of nodes in the superconducting gap ∆(k). The temperature dependence of the superfluid density demonstrates that the gap is non-zero (∆/kBTc 1.6) all over the Fermi surface. The microwave conductivity below Tc exhibits an enhancement larger than the coherence peak, reminiscent of high-Tc cuprate superconductors.PACS numbers: 74.25. Nf, 74.20.Rp, 74.25.Fy Since the discovery of superconductivity in, high transition temperatures (T c ) up to 56 K have been reported in the doped Fe-based oxypnictides [2,3,4,5,6,7,8,9]. The nature of superconductivity and the pairing mechanism in this system are fundamental physical problem of crucial importance. The first experimental task to this problem is to elucidate the superconducting pairing symmetry, which is intimately related to the pairing interaction.The NMR Knight-shift measurements appear to indicate the spin-singlet pairing [10,11]. However, the superconducting gap structure, particulary the presence or absence of nodes in the gap, is highly controversial. The specific heat shows a nonlinear magnetic field dependence [12]. The NMR relaxation rate shows the absence of the coherence peak and the T 3 -dependence below T c [10,11,13,14]. The lower critical field exhibits a T -linear dependence at low temperatures [15]. The µSR experiments report an unusual field-dependence of the penetration depth [16]. In the point-contact spectroscopy, a zero-bias conductance peak is reported [17,18]. These results have been interpretated as an indication of unconvensional superconductivity with line nodes. On the other hand, the Andreev reflection data are found to be consistent with an isotropic gap [19]. All of these experiments have been performed by using polycrystalline samples. Definitely, measurements using single crystals are highly desired to obtain unambiguous conclusions on the superconducting gap structure.In this paper, we report on the measurements of the complex surface impedance in underdoped single crystals of the oxypnictide superconductor PrFeAsO 1−y (T c ≈ 35 K), from which properties of thermally excited quasiparticles can be directly deduced. Since the recent NMR experiments of the Pr-based iron oxypnictide suggest the non-magnetic state in the superconducting samples [11], PrFeAsO 1−y seems suitable for the penetration depth study [20,21]. Moreover, PrFeAsO 1−y has a higher T c than that of La-compounds, which enables the measurements in a wider temperature range. We observe flat temperature dependence of the in-plane penetration depth λ ab (T ) at low temperatures, indicating exponentially small quasiparticle excitations, which clearly contradicts the presence of nodes in the gap. The quasiparticle conductivity is enhanced compared with ...