<sup>31</sup>P spin–lattice and singlet order relaxation mechanisms in pyrophosphate studied by isotopic substitution, field shuttling NMR, and molecular dynamics simulation

Korenchan, David E.; Lu, Jun‐Sheng; Sabba, Mohamed; Dagys, Laurynas; Brown, Lynda J.; Levitt, Malcolm H.; Jerschow, Alexej

doi:10.1039/d2cp03801c

Cited by 9 publications

(6 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Their clinical efficacy and safety have been confirmed by clinical trials. The new DEB has been approved for clinical treatment and research in 2015 [ 37 – 39 ].…”

Section: Callisperes ® Of Drug-loaded Microspherementioning

confidence: 99%

Clinical research progress of callisperes® of drug-loaded microsphere arterial chemoembolisation in the treatment of solid tumors

Wang,

Zhu,

Sheng

2024

Discov Onc

View full text Add to dashboard Cite

The incidence and mortality of cancer is ever-increasing, which poses a significant challengesto human health and a substantial economic burden to patients. At present, chemotherapy is still a primary treatment for various cancers. However, chemotherapy kills tumors but also induces the related side effects, whichadversely impacting patient quality of life and exacerbating suffering. Therefore, there is an urgent need for new and effective treatments that can control tumor growth while reducing the side effects for patients. Arterial chemoembolization has been attracted much attentionwhich attributed to the advantage of ability to embolize tumor vessels to block blood and nutrition supplies. Thus, to achieve local tumor control, it has become an effective means of local tumor control and has been widely used in clinical practice. Despite its efficacy, conventional arterial chemoembolization techniques, limited by embolization materials, have been associated with incomplete embolization and suboptimal drug delivery outcomes. Gradually, researchers have shifted their attention to a new type of embolic material called CalliSperes® drug-eluting embolic bead (DEB). DEB can not only load high doses of drugs, but also has strong sustained drug release ability and good biocompatibility. The integration of DEBs with traditional arterial chemoembolization (DEB-TACE) promises targeted vascular embolization, mitigated tumor ischemia and hypoxia, and direct intravascular chemotherapy delivery. It can prevent cancer cell differentiation and accelerate their death, meanwhile, directly injecting chemotherapy drugs into the target blood vessels reduced the blood concentration of the whole body, thus reduced the toxic and side effects of chemotherapy. Furthermore, DEB-TACE's sustained drug release capability elevates local drug concentrations at the tumor site, amplifying its antitumor efficacy. Therefore, DEB-TACE has become a hot spot in clinical research worldwide. This review introduces the pathogenesis of solid tumors, the background of research and biological characteristics of DEB, and the action mechanism of DEB-TACE, as well as its clinical research in various solid tumors and future prospects. This review aims to provide new ideas for the treatment of DEB-TACE in various solid tumors.

show abstract

“…Their clinical efficacy and safety have been confirmed by clinical trials. The new DEB has been approved for clinical treatment and research in 2015 [ 37 – 39 ].…”

Section: Callisperes ® Of Drug-loaded Microspherementioning

confidence: 99%

Clinical research progress of callisperes® of drug-loaded microsphere arterial chemoembolisation in the treatment of solid tumors

Wang,

Zhu,

Sheng

2024

Discov Onc

View full text Add to dashboard Cite

show abstract

“…Fluorescence and nuclear magnetic resonance (NMR) spectroscopy have been used to study conformational exchange on time scales spanning several orders of magnitude by monitoring the lifetimes of nonequilibrium electronic or nuclear spin states. 15,16 Previous studies investigated the changes in time-resolved fluorescence emission 17−19 and/or the NMR spectral changes 20−23 of NAD + /NADH in pure solvents such as water or methanol.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Recent spectroscopic and structural evidence point toward a preference for the unfolded state when binding to the active sites of enzymes which allows for stronger host–guest interactions due to the increased molecular surface. − As a result, perturbations of the freely available, unbounded U/F species arising from temperature, , pH, , solvent, , or chemical gradients , can impact the global enzymatic rates of energy metabolism leading to cellular malfunction. Fluorescence and nuclear magnetic resonance (NMR) spectroscopy have been used to study conformational exchange on time scales spanning several orders of magnitude by monitoring the lifetimes of nonequilibrium electronic or nuclear spin states. , Previous studies investigated the changes in time-resolved fluorescence emission − and/or the NMR spectral changes − of NAD + /NADH in pure solvents such as water or methanol.…”

Section: Introductionmentioning

confidence: 99%

Conformational Landscape of NADH and Ion Binding in Water/DMSO Mixtures via ³¹P NMR Spectroscopy

Lu,

Teleanu,

Zou

et al. 2024

J. Phys. Chem. B

View full text Add to dashboard Cite

This study reports on the conformational states of nicotinamide adenine dinucleotide (NADH) in water/DMSO mixtures and examines the influence of ion binding. We observe evidence of conformational changes through a series of NMR techniques, including 31 P NMR relaxation (R 1 and R 2 ), chemical exchange saturation transfer (CEST), and diffusion-ordered spectroscopy (DOSY) experiments. The observed variation of the conformational states is indicative of the solvent's influence on NADH's structural flexibility. The experimental findings, in combination with viscosity data, are shown to be in line with findings from earlier molecular dynamics studies. The reported observations emphasize the critical role of the solvent environment in determining the conformational states of NADH and similar molecules with relevance for the biophysiological context. The results found herein can help in studying the biophysical behavior of NADH and similar biomolecules and their associated metabolic pathways under various solvent conditions.

show abstract

“…An upper bound of 37 min has been estimated for the entanglement lifetime of Posner molecules (Ca 9 (PO 4 ) 6 ) formed by the hydrolysis of pyrophosphate . In addition, pyrophosphate ions have been encoded to the singlet state using partially 18 O labeled samples . Therefore, real-time monitoring of the hydrolysis for the encoded pyrophosphate ions to clarify this conjecture is important for quantum physics, information, biology, and life sciences.…”

mentioning

confidence: 99%

“…26 In addition, pyrophosphate ions have been encoded to the singlet state using partially 18 O labeled samples. 27 Therefore, real-time monitoring of the hydrolysis for the encoded pyrophosphate ions to clarify this conjecture is important for quantum physics, information, biology, and life sciences. Phosphorus ( 31 P) spin using d-DNP has been studied such as in the pH imaging of phosphate solutions due to chemical shift changes 28 and the growth of calcium phosphate crystals.…”

mentioning

confidence: 99%

Real-Time Monitoring of Hydrolysis Reactions of Pyrophosphates with Dissolution Dynamic Nuclear Polarization

Fukazawa,

Mochizuki,

Kanai

et al. 2024

J. Phys. Chem. Lett.

View full text Add to dashboard Cite

Dissolution dynamic nuclear polarization (d-DNP) has enabled applications such as the real-time monitoring of chemical reactions. Such applications are mainly for 13 C and 15 N spins with long spin−lattice relaxation times in the molecules of interest. However, the only applications for phosphorus using d-DNP are pH imaging and nucleation during crystallization due to the short relaxation times. Here we show that it is possible to observe enzyme reactions using d-DNP with phosphorus. Hyperpolarized 31 P spins in pyrophosphate were obtained using bullet-DNP, which requires less dilution of highly polarized solid samples. Real-time monitoring of the hydrolysis reaction of pyrophosphate by inorganic pyrophosphatase from baker's yeast at physiological pH and was successfully achieved and the reaction rate was determined. This is an important reaction for a wide range of applications related to medicine, agriculture, and quantum life science.

show abstract

³¹P spin–lattice and singlet order relaxation mechanisms in pyrophosphate studied by isotopic substitution, field shuttling NMR, and molecular dynamics simulation

Abstract: Nuclear spin relaxation mechanisms are often difficult to isolate and identify, especially in molecules with internal flexibility. Here we combine experimental work with computation in order to determine the major...

Cited by 9 publications

References 53 publications

Clinical research progress of callisperes® of drug-loaded microsphere arterial chemoembolisation in the treatment of solid tumors

Clinical research progress of callisperes® of drug-loaded microsphere arterial chemoembolisation in the treatment of solid tumors

Conformational Landscape of NADH and Ion Binding in Water/DMSO Mixtures via ³¹P NMR Spectroscopy

Real-Time Monitoring of Hydrolysis Reactions of Pyrophosphates with Dissolution Dynamic Nuclear Polarization

Contact Info

Product

Resources

About

31P spin–lattice and singlet order relaxation mechanisms in pyrophosphate studied by isotopic substitution, field shuttling NMR, and molecular dynamics simulation

Abstract: Nuclear spin relaxation mechanisms are often difficult to isolate and identify, especially in molecules with internal flexibility. Here we combine experimental work with computation in order to determine the major...

Cited by 9 publications

References 53 publications

Clinical research progress of callisperes® of drug-loaded microsphere arterial chemoembolisation in the treatment of solid tumors

Clinical research progress of callisperes® of drug-loaded microsphere arterial chemoembolisation in the treatment of solid tumors

Conformational Landscape of NADH and Ion Binding in Water/DMSO Mixtures via 31P NMR Spectroscopy

Real-Time Monitoring of Hydrolysis Reactions of Pyrophosphates with Dissolution Dynamic Nuclear Polarization

Contact Info

Product

Resources

About

³¹P spin–lattice and singlet order relaxation mechanisms in pyrophosphate studied by isotopic substitution, field shuttling NMR, and molecular dynamics simulation

Conformational Landscape of NADH and Ion Binding in Water/DMSO Mixtures via ³¹P NMR Spectroscopy