<sup>31</sup>P NMR saturation transfer study of the creatine kinase reaction in human skeletal muscle at rest and during exercise

Goudemant, Jean-François; Francaux, Marc; Mottet, Isabelle; Demeure, Roger; Sibomana, M.; Sturbois, Xavier

doi:10.1002/mrm.1910370518

Cited by 29 publications

(34 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Relevant kinetic parameters characterizing the CK reaction in resting human skeletal muscle in both young, healthy volunteers and the patient are summarized in Table 3. Parameters describing flux through the forward CK reaction agree well with findings by other researchers, who did not assess the reverse reaction [17,18]. Consistent with animal studies, the ratio k −1 /k +1 is roughly three [14,15].…”

Section: Application To Human Skeletal Musclesupporting

confidence: 87%

“…Early examples include the adenylate kinase [10] and the ATPase reaction [11]. Flux through the CK reaction has been most extensively investigated by this approach including perfused organs [12,13], whole animals [14][15][16], and human subjects [7,[17][18][19][20][21][22]. The following discussion will focus on experiments designed to study the CK reaction in resting human skeletal muscle.…”

Section: Biochemical Considerationsmentioning

confidence: 99%

See 1 more Smart Citation

Magnetization‒transfer ³¹P NMR of biochemical exchange in vivo: Application to creatine kinase kinetics

Möller¹,

Wiedermann²

2002

Journal of Spectroscopy

View full text Add to dashboard Cite

Abstract. Phosphorus-31 saturation-transfer NMR spectroscopy provides an elegant means to study fluxes through the creatine kinase reaction in human skeletal muscle. To obtain reliable quantitative kinetic information, experimental imperfections, such as incomplete saturation and radiofrequency bleed over need to be addressed appropriately. In resting muscle, creatine kinase was near equilibrium both in normal controls and in a patient with impaired oxidative phosphorylation. Oral intake of high doses of creatine monohydrate for several days resulted in significantly increased concentrations of phosphocreatine but had no measurable effect on the phosphocreatine resynthesis rate in resting muscle.Abbreviations ADP = adenosine 5 -diphosphate; ATP = adenosine 5 -triphosphate; CK = creatine kinase; Cr = creatine; MEALS = mitochondrial myopathy, encephalopathy, lactic acidosis, and stroke-like episodes; NMR = nuclear magnetic resonance; PCr = phosphocreatine; PDE = phosphodiesters; P i = inorganic phosphate; PME = phosphomonoesters; PP = phosphorylation potential; RF = radiofrequency; ST = saturation transfer.

show abstract

Section: Application To Human Skeletal Musclesupporting

confidence: 87%

Section: Biochemical Considerationsmentioning

confidence: 99%

Magnetization‒transfer ³¹P NMR of biochemical exchange in vivo: Application to creatine kinase kinetics

Möller¹,

Wiedermann²

2002

Journal of Spectroscopy

View full text Add to dashboard Cite

show abstract

“…The [PC r ] values range from 17.8 to 37.7 mmol/kg [1,7,11,13,15,22,23]. The reasons for this great variability are discussed in [12].…”

Section: Data and Model: Facts And Theorymentioning

confidence: 95%

Energetics of muscular exercise at work onset: the steady-state approach

Prampero

Francescato

Cettolo

2003

Pflugers Arch - Eur J Physiol

View full text Add to dashboard Cite

The energetics of muscular exercise at steady state is tightly coupled to, and dependent on, the events that occurred during the transient phase, of which the steady-state is therefore the "memory". The aim of the present study is to show that it is possible to utilize data obtained at exercise steady state to gain information on variables traditionally assessed during exercise transients. A theoretical model based on the steady-state relationships between mechanical power, O(2) uptake (VO(2)) and phosphocreatine (PC) split allows us to highlight three interdependent parameters: the time constant of the VO(2) on response at the muscular level (tau), the mechanical equivalent of PC splitting and the P/O(2) ratio. The model was applied to experimental data obtained during moderate calf exercise in humans inside an MR unit. For a P/O(2) from 5.6 to 6.2, the obtained tau values range from 10.6 to 24.9 s (for PC concentrations from 17.8 to 37.7 mmol/kg fresh muscle), and the corresponding mechanical equivalents of PC from 9.6 to 10.6 J/mmol. The analysis also shows that the time constant of the O(2) uptake kinetics is strictly dependent on the PC concentration at rest, whereas the mechanical equivalent of PC is unaffected by its concentration.

show abstract

“…Notwithstanding species and experimental differences the degree to which compartmentalisation occurs in the fibre (e.g. Seraydarian et al 1962;Goudemant et al 1997) during maximal exercise may give rise to localised depletion of PCr.…”

Section: Discussionmentioning

confidence: 99%

Metabolic Changes in Single Human Muscle Fibres During Brief Maximal Exercise

Karatzaferi

Haan

Mechelen³

et al. 2001

Experimental Physiology

View full text Add to dashboard Cite

Changes in high‐energy phosphate levels in single human skeletal muscle fibres after 10 s of maximal (all‐out) dynamic exercise were investigated. Muscle biopsies from vastus lateralis of two volunteers were collected at rest and immediately post exercise. Single muscle fibres were dissected from dry muscle and were assigned into one of four groups according to their myosin heavy chain (MyHC) isoform content: that is type I, IIA, IIAx and IIXa (the latter two groups containing either less or more than 50% IIX MyHC). Fragments of characterised fibres were analysed by HPLC for ATP, inosine‐monophosphate (IMP), phosphocreatine (PCr) and creatine levels. After 10 s of exercise, PCr content ([PCr]) declined by approximately 46, 53, 62 and 59% in type I, IIA, IIAx and IIXa fibres, respectively (P < 0.01 from rest). [ATP] declined only in type II fibres, especially in IIAx and IIXa fibres in which [IMP] reached mean values of 16 ± 1 and 18 ± 4 mmol (kg dry mass)−1, respectively. While [PCr] was reduced in all fibre types during the brief maximal dynamic exercise, it was apparent that type II fibres expressing the IIX myosin heavy chain isoform were under a greatest metabolic stress as indicated by the reductions in [ATP].

show abstract

³¹P NMR saturation transfer study of the creatine kinase reaction in human skeletal muscle at rest and during exercise

Cited by 29 publications

References 49 publications

Magnetization‒transfer ³¹P NMR of biochemical exchange in vivo: Application to creatine kinase kinetics

Magnetization‒transfer ³¹P NMR of biochemical exchange in vivo: Application to creatine kinase kinetics

Energetics of muscular exercise at work onset: the steady-state approach

Metabolic Changes in Single Human Muscle Fibres During Brief Maximal Exercise

Contact Info

Product

Resources

About

31P NMR saturation transfer study of the creatine kinase reaction in human skeletal muscle at rest and during exercise

Cited by 29 publications

References 49 publications

Magnetization‒transfer 31P NMR of biochemical exchange in vivo: Application to creatine kinase kinetics

Magnetization‒transfer 31P NMR of biochemical exchange in vivo: Application to creatine kinase kinetics

Energetics of muscular exercise at work onset: the steady-state approach

Metabolic Changes in Single Human Muscle Fibres During Brief Maximal Exercise

Contact Info

Product

Resources

About

³¹P NMR saturation transfer study of the creatine kinase reaction in human skeletal muscle at rest and during exercise

Magnetization‒transfer ³¹P NMR of biochemical exchange in vivo: Application to creatine kinase kinetics

Magnetization‒transfer ³¹P NMR of biochemical exchange in vivo: Application to creatine kinase kinetics