<sup>13</sup>C nuclear magnetic resonance spectroscopy of glucose metabolism in human red blood cells

Ghai, Geetha; Gonnella, Nina C.

doi:10.1002/mrm.1910080311

Cited by 4 publications

(1 citation statement)

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“…13C-enrichment of C3-2,3-DPG was also detected (Pig 1. resonance at c 67.3 ppm). During the first 3 h following the addition of 1-1 3C-glucose, minor 3C-labelling of the C6-and C4-glucose and C3-glucose/-glucose-6phosphate peaks, near 61, 70 and 76ppm, respectively, were alsci observed, indicating that the samples were adequately oxygenated (see Ghai & Gonnella, 1988). Timecourse data of 1-1 3C-glucose consumption and 3-13C-lactate production for all three erythrocyte types are shown in Fig 2. Linear best fit analysis of the kinetics of 1-1 3C-glucose consumption showed that thalassaemia intermedia erythrocytes metabolized glucose almost 3 times faster than both normal and heterozygous red blood cells.…”

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confidence: 99%

In vivo metabolic studies of glucose, ATP and 2,3‐DPG in β‐thalassaemia intermedia, heterozygous β‐thalassaemic and normal erythrocytes: ¹³C and ³¹P MRS studies

et al. 1994

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13C and 31P magnetic resonance spectroscopy was used to characterize the in vivo kinetics of glucose metabolism and intracellular ATP and 2,3-DPG concentrations in erythrocytes obtained from beta-thalassaemia intermedia, heterozygous beta-thalassaemic and normal individuals and maintained in suspension. Except for an upfield chemical shift in the 2P and 3P resonance of 2,3-DPG in the thalassaemia intermedia erythrocytes, the 31P spectra were comparable between all three blood types, showing similar concentrations of ATP (from 4.5 to 5.2 mumol/g Hb) and 2,3-DPG (from 17.2 to 19.7 mumol/g Hb). However, the profile of glucose metabolism was quite different in beta-thalassaemia intermedia erythrocytes, whereas glucose was consumed at a rate of 0.089 +/- 0.035 fmol/cell/h, significantly higher than that of normal (0.032 +/- 0.018 fmol/cell/h; P = 0.01) and heterozygous (0.025 +/- 0.004 fmol/cell/h; P = 0.01) erythrocytes. This near 3-fold faster rate of glucose metabolism in the thalassaemia intermedia erythrocytes could not be accounted for by any increase in glucose flux via the Embden-Meyerhof pathway, since no significant difference in 3-13C-lactate synthesis was observed among the three blood types (in units of fmol/cell/h, normal, 0.021 +/- 0.013; heterozygous, 0.021 +/- 0.006; beta-thalassaemia intermedia 0.045 +/- 0.025). These results reflect an accelerated rate of glucose metabolism in thalassaemia intermedia erythrocytes because the contribution of reticulocytes to this altered pattern of metabolism could be excluded. As the only other route of glucose metabolism in erythrocytes is the pentose phosphate pathway (PPP), these results indicate that the PPP is more active in beta-thalassaemia intermedia erythrocytes, perhaps as a consequence of their elevated intracellular oxidative state.

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Section: Sample Preparationmentioning

confidence: 99%

In vivo metabolic studies of glucose, ATP and 2,3‐DPG in β‐thalassaemia intermedia, heterozygous β‐thalassaemic and normal erythrocytes: ¹³C and ³¹P MRS studies

et al. 1994

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Study on the erythrocytes from myotonic dystrophy with multi‐nuclear NMR

et al. 1991

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We have studied the water permeability through membranes, the function of the Na pump, and glucose metabolism of erythrocytes of patients with myotonic muscular dystrophy (MyD) using 1H--, 23Na, and 13C-NMR techniques. A significant decrease in water permeability was recognized in the MyD erythrocyte membrane, and impaired Na pumping was suspected to be correlated with the former biochemical abnormalities in band III protein of MyD erythrocyte membrane. Significant acceleration of glycolysis in the erythrocyte for the first 160 minutes was also recognized in MyD; however, the production of lactate showed no difference between MyD and controls. The increased glucose uptake in MyD may be compensatory to the diminished pumping mechanism, but further information, such as inorganic phosphate permeability and the activity of the rate-limiting enzyme of erythrocyte glycolysis, is needed.

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13C and 31P NMR studies of glucose and 2-deoxyglucose metabolism in normal and enzyme-deficient human erythrocytes

Ferretti¹,

Bozzi²,

Vito³

et al. 1992

Clinica Chimica Acta

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¹³C nuclear magnetic resonance spectroscopy of glucose metabolism in human red blood cells

Cited by 4 publications

References 10 publications

In vivo metabolic studies of glucose, ATP and 2,3‐DPG in β‐thalassaemia intermedia, heterozygous β‐thalassaemic and normal erythrocytes: ¹³C and ³¹P MRS studies

In vivo metabolic studies of glucose, ATP and 2,3‐DPG in β‐thalassaemia intermedia, heterozygous β‐thalassaemic and normal erythrocytes: ¹³C and ³¹P MRS studies

Study on the erythrocytes from myotonic dystrophy with multi‐nuclear NMR

13C and 31P NMR studies of glucose and 2-deoxyglucose metabolism in normal and enzyme-deficient human erythrocytes

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13C nuclear magnetic resonance spectroscopy of glucose metabolism in human red blood cells

Cited by 4 publications

References 10 publications

In vivo metabolic studies of glucose, ATP and 2,3‐DPG in β‐thalassaemia intermedia, heterozygous β‐thalassaemic and normal erythrocytes: 13C and 31P MRS studies

In vivo metabolic studies of glucose, ATP and 2,3‐DPG in β‐thalassaemia intermedia, heterozygous β‐thalassaemic and normal erythrocytes: 13C and 31P MRS studies

Study on the erythrocytes from myotonic dystrophy with multi‐nuclear NMR

13C and 31P NMR studies of glucose and 2-deoxyglucose metabolism in normal and enzyme-deficient human erythrocytes

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Product

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¹³C nuclear magnetic resonance spectroscopy of glucose metabolism in human red blood cells

In vivo metabolic studies of glucose, ATP and 2,3‐DPG in β‐thalassaemia intermedia, heterozygous β‐thalassaemic and normal erythrocytes: ¹³C and ³¹P MRS studies

In vivo metabolic studies of glucose, ATP and 2,3‐DPG in β‐thalassaemia intermedia, heterozygous β‐thalassaemic and normal erythrocytes: ¹³C and ³¹P MRS studies