<sup>13</sup>C‐nmr spectroscopic investigation of two yeast cell wall β‐<scp>D</scp>‐glucans

Kogan, Grigorij; Alföldi, Juraj; Masler, L.

doi:10.1002/bip.360270702

Cited by 57 publications

(19 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The disordered conformation of the L-FV-IA in DMSO is also similar to schizophyllan and Lentinan in 0.38 M NaOH aqueous solution. 10 15 and Schzophyllan in 0.06 M NaOD/D 2 O. 10 Moreover, with an increase of water content the relative intensities of C6 signal at 61 ppm significantly decreased and shifted to downfield by 1.2 ppm.…”

Section: Resultsmentioning

confidence: 95%

“…14 The gel-forming ability of polysaccharide is of considerable importance in view of their biological functions. 15 Moreover, helixcoil transitions are important in biology, as they appear in proteins and DNA. 16 In this work, the conformation transition for the β-(1 → 3)-D-glucan (Lentinan) from Lentinus edodes in water/DMSO mixture solution was investigated by laser light scattering, size exclusion chromatography, viscometry and 13 C NMR.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Transition from Triple Helix to Coil of Lentinan in Solution Measured by SEC, Viscometry, and 13C NMR

Zhang

Zhou

et al. 2002

Polym J

View full text Add to dashboard Cite

ABSTRACT:Lentinan, β-(1 → 3)-D-glucan with (1 → 6) branching, was isolated from Lentinus edodes. Weightaverage molecular weight M w , radius of gyration < s 2 > 1/2 z and intrinsic viscosity [η] of Lentinan in 0.2 M NaCl aqueous solution, dimethylsulfoxide (DMSO) and water/DMSO mixtures were measured by light scattering (LS), size exclusion chromatography (SEC) combined with LS, and viscometry. The results indicated that the glucan exists mainly as triple-helical chains in 0.2 M NaCl aqueous solution and water/DMSO mixtures with over 20 wt% water content, and as single-flexible chain in DMSO. The data from SEC-LS, viscosity and 13 C NMR measurements proved strongly that the helix-coil conformation transition occurred in a narrow range from 80 to 85 wt% DMSO aqueous solution, accompanying with obvious changes of M w , < s Chihara et al. have reported that the β-(1 → 3)-Dglucan, Lentinan, obtained from Lentinus edodes has a strong antitumour activity against sarcoma 180 implanted subcutaneous in mice. 1 Thereby, the importance of the polysaccharides has provided a major impetus for increasing attention. Particularly mushroom polysaccharides have been used as functional food, and are developed in the field of new drugs. 2 The structural analysis may offer the most fundamental knowledge to understand the functions of polysaccharide, but the diversity and irregularity of polysaccharide chain make the structural analysis a formidable task. 3 Although a monosaccharide unit is common to many polysaccharides, its linkage mode varies and characteristic functions properties will appear accordingly. It is worth noting that polysaccharides in aqueous solution exhibit different chain conformation such as single helix, 4 triple helix, 5 random coil, 6 and aggregate, 7 even if their backbone all are β-(1 → 3)-D-glucan. However, conformation and its transition from an ordered state to a disordered for β-(1 → 3)-D-glucan in solution have not been fully investigated.Helix conformations of β-(1 → 3)-D-glucan with two (1 → 6)-glucosyl side groups for every five residues isolated from Lentinus edodes have been reported, based on analysis from the results of X-Ray diffraction 8 and 13 C NMR. 9-12 However, it has long been suspected to be a triple helix of the β-D-glucan in aqueous solution without strong evidence. In our recent work, 13 experimental results from light scattering have indicated that the predominant species of the β-D-glucan in 0.5 M NaCl aqueous solution exist as triple-helical chains with high rigidity, and in dimethylsulfoxide (DMSO) as single-flexible chains. The single or multiple helices of biopolymers in aqueous solution aggregate easily to form gel. The gelation and the helical state may be induced by lowering the temperature, by changing the solvent, or by adding salt. 14 The gel-forming ability of polysaccharide is of considerable importance in view of their biological functions. 15 Moreover, helixcoil transitions are important in biology, as they appear in proteins and DNA. 16 In this work, the conformation ...

show abstract

Section: Resultsmentioning

confidence: 95%

mentioning

confidence: 99%

Transition from Triple Helix to Coil of Lentinan in Solution Measured by SEC, Viscometry, and 13C NMR

Zhang

Zhou

et al. 2002

Polym J

View full text Add to dashboard Cite

show abstract

“…2B). The spectrum is characteristic of a strictly linear (133)-␤-D-glucan for which resonance peaks are expected at 61.0, 68.5, 72.9, 76.4, 86.3, and 103.1 ppm for C6, C4, C2, C5, C3, and C1, respectively (33,54,62). It is comparable to those obtained for wellcharacterized (133)-␤-D-glucans (33,62) and for in vitro (133)-␤-D-glucans synthesized by enzymes from S. monoica (54) and plants (14,36,37).…”

Section: Results (133)-␤-d-glucan and Chitin Synthases Are Located Inmentioning

confidence: 99%

Cell Wall Polysaccharide Synthases Are Located in Detergent-Resistant Membrane Microdomains in Oomycetes

Briolay

Bouzenzana

Guichardant

et al. 2009

Appl Environ Microbiol

View full text Add to dashboard Cite

“…Fungal glucan was isolated from the cell wall of Saccharomyces cerevisiae as previously described (Kogan et al 1988, Chorvatovičová et al 1993. Candidate antagonistic Trichoderma strains from the collection of ISPA, Bari, Italy were tested against pathogenic Fusarium species isolated from maize ears and roots in dual cultures grown on potato-dextrose-agar (Sigma) for 7-21 days.…”

Section: Methodsmentioning

confidence: 99%

Trichoderma and sulphoethyl glucan reduce maize root rot infestation and fusaric acid content

Śrobárová¹,

Eged²

2005

PLANT SOIL ENVIRON

View full text Add to dashboard Cite

Roots of maize seedlings (cv. Pavla) infested by Fusarium verticillioides (10 5 /ml) were cultivated on Murashige-Skoog medium (MSM, Sigma, USA) containing CaCl 2 , IAA and kinetin. Simultaneously, a strain of the antagonistic fungus Trichoderma sp. and a sulphoethyl glucan (SEG) isolated from the cell walls of Saccharomyces cerevisiae, were added. Two evaluations (on 7 and 14 days) were done. Productivity parameters of leaves and roots (fwt, dwt, and length), disease severity index (DSI) and fusaric acid (FA) concentration were evaluated. Both Trichoderma sp. and SEG increased productivity parameters of plants in infested variants and maintained it on the level of control plants during 14 days of experiment. Trichoderma reduced the DSI, while SEG increased it. DSI correlated with FA concentration. A�er seven days of cultivation concentration of FA was lower in all infected variants cultivated concomitantly with agents, compared with the one without them. A�er 14 days of cultivation both agents reduced the concentration of FA up to 50% to the non-measurable concentration in variant with Trichoderma. In variant with positive control, where FA was added to SEG, its concentration decreased up to 30%. 1. growth medium, control 2. F. verticillioides 10 5 /ml spore concentration 3. F. verticillioides 10 5 /ml spore concentration with Trichoderma sp.10 6 /ml 4. F. verticillioides 10 5 /ml spore concentration with 0.025% SEG 5. Trichoderma sp. 10 6 /ml 6. 0.025% SEG After 7 days of cultivation as a toxin positive control 7) variant with 0.025% SEG and fusaric acid (FA) in a concentration 8.40 µg/ml was added according to the average level evaluated in situ samples (Nadubinská et al. 2002).In each variant 15 three-days-old seedlings were inserted, in two repetitions. All variants had volume of 200 ml and after 7 days of cultivation a fresh solution of MSM was added to maintain constant volume. We used the strain of F. verticillioides which produced FA in vitro. Plants were cultivated hydroponically in a special room under controlled conditions at temperature of 21/15°C day/night and 16/8 h photoperiod provided by white fluorescent lights, irradiance 180 µmol/m 2 /s, day/night relative humidity 80/60%.Evaluation of experimental results. After 7 and 14 days, the roots were separated from the leaves and evaluated for the degree of infestation, the content of FA, and productivity parameters: fresh weight (fwt), dry weight (dwt), and length. The longest roots and leaves were measured in each plant. To determine the dry weight, leaves and roots were dried separately at 105°C to constant weight. The data were subjected to standard statistical analysis, using SAS program 6. 08 (1990). The means where applicable were tested for significant differences, using the LSD (P < 0.05).Disease severity. The disease severity index (DSI) was rated on the 14 th day using a scale according to Kroon and Elgersma (1993) in plants of each treatment, using a 0 -5 disease index, where 0 indicated a healthy plant; 1 implied penalty of leave...

show abstract

¹³C‐nmr spectroscopic investigation of two yeast cell wall β‐D‐glucans

Cited by 57 publications

References 18 publications

Transition from Triple Helix to Coil of Lentinan in Solution Measured by SEC, Viscometry, and 13C NMR

Transition from Triple Helix to Coil of Lentinan in Solution Measured by SEC, Viscometry, and 13C NMR

Cell Wall Polysaccharide Synthases Are Located in Detergent-Resistant Membrane Microdomains in Oomycetes

Trichoderma and sulphoethyl glucan reduce maize root rot infestation and fusaric acid content

Contact Info

Product

Resources

About

13C‐nmr spectroscopic investigation of two yeast cell wall β‐D‐glucans

Cited by 57 publications

References 18 publications

Transition from Triple Helix to Coil of Lentinan in Solution Measured by SEC, Viscometry, and 13C NMR

Transition from Triple Helix to Coil of Lentinan in Solution Measured by SEC, Viscometry, and 13C NMR

Cell Wall Polysaccharide Synthases Are Located in Detergent-Resistant Membrane Microdomains in Oomycetes

Trichoderma and sulphoethyl glucan reduce maize root rot infestation and fusaric acid content

Contact Info

Product

Resources

About

¹³C‐nmr spectroscopic investigation of two yeast cell wall β‐D‐glucans