¹H NMR study of cerebral development in the rat

Abstract

1H NMR spectroscopy of brain extracts was used to investigate the metabolic changes that take place during development of the neonatal rat brain. Data were obtained over the range 1-21 days. The concentration of N-acetylaspartate rose by a factor of 9 during this period, the most rapid rise occurring after day 9. The total creatine concentration rose from days 1-21, with a large increase between days 1 and 5. Taurine concentration rose until day 5, then fell from days 5-21. The concentration of choline-containing compounds fell during the 21 day period. The results are discussed in relation to brain development and conventional biochemical data. A major conclusion in relation to spectroscopy of children is that interpretation of changes seen in disease will require adequate data from age-matched controls.

Early and Sustained Alterations in Cerebral Metabolism after Traumatic Brain Injury in Immature Rats

“…It is a postulated source of acetate for lipid and myelin synthesis of oligodendrocytes (Satrustegui et al, 2007). As the immature rat brain develops, rapid increases in NAA are detected during the time of peak myelination (D'Adamo and Yatsu, 1966;Bates et al, 1989;Burri et al, 1990). Using purified rat brain mitochondria (Patel and Clark, 1979), the synthesis and transport of NAA out of mitochondria increased with brain development in concert with myelination.…”

Metabolic studies of human primitive neuroectodermal tumour cells by proton nuclear magnetic resonance spectroscopy

“…Additionally, Urenjak et al (1993) have found that the qualitative and quantitative differences between 'H-NMR spectra obtained from different purified populations of neural cells appears to be strongly correlated with the lineage of the cell type examined. In this respect, the higher levels of NAA found in less-differentiated PNETs and Miyake and Kakimoto (1981) and Bates et al (1989), the relative contribution of the neuronal and 0-2A lineages to this rise is unclear. Therefore, it is not possible to say whether the NAA levels observed in PNET cell lines are typical of dividing neuronal progenitors of the cerebellum.…”

In vivo detection of metabolic changes in a mouse model of scrapie using nuclear magnetic resonance spectroscopy

“…In vivo proton NMR spectroscopy methods have been used recently to investigate cerebral metabolism in human subjects, both in healthy adults (Frahm et al, 1990), and in patients presenting with focal lesions (Gill et al, 1990;Wolinsky et al, 1990) and diffuse disorders (Menon et al, 1990a, b). Metabolic processes have also been studied in a wide variety of animal models of the disease (Gadian et al, 1986;Bates et al, 1989b;Prichard & Shulman, 1986) and perfused organs (Cohen, 1983;Hollis, 1982), and in simpler systems such as isolated cell suspensions (Hollis, 1982;Navon et al, 1983), tissue extracts (Bates et al, 1989a;Gill et al, 1990) and body fluids (Bell et aL, 1989).…”