¹H NMR quantification of cannabidiol (CBD) in industrial products derived from Cannabis sativa L. (hemp) seeds

Metabolomic Profile and Antioxidant/Anti-Inflammatory Effects of Industrial Hemp Water Extract in Fibroblasts, Keratinocytes and Isolated Mouse Skin Specimens

“…The presence of CBD was also confirmed by 1 H-NMR analysis. In this context, the water hemp spectrum was compared to CBD standard solution spectrum, giving, in particular, specific chemical shift signals at 4.546, 4.655 and 6.177 ppm, in CDCL 3 ( Figure 3 ), that are consistent with literature [ 28 ]. The phytochemical composition of the extract also agrees with antioxidant, anti-inflammatory and antimicrobial activities [ 8 ].…”

A Quantitative 1H-NMR Method for Screening Cannabinoids in CBD Oils

“…In addition, the chemical shifts of these signals were strongly temperature-dependent (see Figure 3). This observation has already been described by several research groups [9,20,35]. The effect of the broadening is attributed in the literature to restricted rotation of the single bond between the phenyl carbon C-1' and the C-1 carbon of the terpene moiety [9,35].…”

“…Prior to the NMR experiments, the samples were dissolved in either pure solvent (CD3OD, CDCl3) or solvent mixtures (CDCl3/CD3OD (3:2, 2:1, v:v) and CDCl3/DMSO-d6 (5:1, v:v)). These solvents and solvent mixtures were previously proposed in the literature for NMR measurement of hemp extracts or edible oils [9,19,20,[22][23][24][25]. To assign the cannabinoid signals in the 1 H NMR spectrum of CBD oils, reference spectra of different cannabinoids were recorded in CDCl3 and their compoundspecific coupling constants, multiplicities, and chemical shift were recorded and verified with literature data.…”

A Quantitative 1H NMR Method for Screening Cannabinoids in CBD Oils

“…In addition to shorter measurement times compared to conventional chromatographic analytical methods, direct analysis by NMR can avoid time-consuming chemical and physical processing. This avoids possible changes in sample composition and analyte losses during sample preparation [19,20]. Furthermore, NMR methods allow simultaneous determination of multiple analytes.…”

“…Furthermore, NMR methods allow simultaneous determination of multiple analytes. Despite these potential advantages, NMR methods have only been rarely employed in the determination of cannabinoids: beside the authentication of Cannabis sativa L. hemp varieties [21,22], 1 H NMR has currently been described so far by only a few working groups to quantify cannabinoids in extracts of the hemp plant and hemp flowers [20,23]. The less sensitive 13 C NMR was also used by Marchetti et al to quantify CBD, CBDA, CBG and cannabigerolic acid (CBGA) in hemp extracts [19].…”