¹H-NMR-based metabolomic studies of bisphenol A in zebrafish (Danio rerio)

Abstract

Proton nuclear magnetic resonance (H-NMR) spectroscopy was used to study the response of zebrafish (Danio rerio) to increasing concentrations of bisphenol A (4,4'-(propane-2,2-diyl)diphenol, BPA). Orthogonal partial least squares discriminant analysis (OPLS-DA) was applied to detect aberrant metabolomic profiles after 72 h of BPA exposure at all levels tested (0.01, 0.1, and 1.0 mg/L). The OPLS-DA score plots showed that BPA exposure caused significant alterations in the metabolome. The metabolomic changes in response to BPA exposure generally exhibited nonlinear patterns, with the exception of reduced levels of several metabolites, including glutamine, inosine, lactate, and succinate. As the level of BPA exposure increased, individual metabolite patterns indicated that the zebrafish metabolome was subjected to severe oxidative stress. Interestingly, ATP levels increased significantly at all levels of BPA exposure. In the present study, we demonstrated the applicability of H-NMR-based metabolomics to identify the discrete nature of metabolic changes.

Metabolomics Reveals That Bisphenol Pollutants Impair Protein Synthesis-Related Pathways in Daphnia magna

“…Acute impairment of these biochemical pathways may consequently alter protein synthesis [57,69]. Similar patterns of impairment to protein synthesis (via branched-chain amino acids and aromatic amino acid metabolism) were observed in zebrafish after exposure to BPA [70]. Our results, therefore, suggest that previously reported mechanisms for BPA exposure are also occurring with BPS sub-lethal exposure.…”

Multi-Omics Sequencing Provides Insights Into Age-Dependent Susceptibility of Grass Carp (Ctenopharyngodon idellus) to Reovirus

“…The downregulation of these pathways in FMO fish implies that the host translation machinery is hjjacked or shut down by GCRV to facilitate the replication and spread of the virus. Similarly, the nucleotide metabolism-related pathways were downregulated in classical swine fever virus-infected piglets (43), and purine metabolism was downregulated in bisphenol A-treated zebrafish (44,45). Collectively, these results show the vital role of nucleotide metabolism in response to virus infection or toxicity stimulation.…”

Perturbations in Amino Acid Metabolism in Reserpine-Treated Zebrafish Brain Detected by1H Nuclear Magnetic Resonance-Based Metabolomics

“…Open-field test was conducted, individually, on four groups of fish, comprising the control group and three reserpineinjected groups (20,40, and 80 mg/kg), to assess their movement and behavior. Each group consisted of 10 fish (n = 10, 5 male and 5 female) placed in a test tank measuring 20 • 8 • 18 cm (length • width • depth).…”

“…Furthermore, the sample size was based on previously reported studies. [19][20][21] The behavioral analysis was done according to Cachat et al with minor modifications. 22 The fish were video recorded and analyzed over a 6-min period using Zebralab Software (Viewpoint) and Smart v3.0, Harvard Apparatus behavioral analysis software.…”