¹H and ¹³C NMR assignments of eight nitrogen containing compounds from Nocardia alba sp.nov (YIM 30243^T)

Abstract

An unprecedented new natural product named nocarsin A (1), 5H-4a,6,7a-triazacyclopenta[cd]indene-5,7(6H)-dione (1), together with seven known compounds lumichrome (2), cyclo (L-Leu-L-Tyr) (3), cyclo (L-Ala-L-Ile) (4), cyclo (L-Ala-L-Leu) (5), cyclo (L-Val-L-Ala) (6), 5-methyluracil (7) and uracil (8), was isolated from Nocardia alba sp.nov (YIM 30243(T)), which was isolated from a soil sample collected from Yunnan Province, P. R. China. NMR techniques including COSY, HSQC, ROESY, and HMBC were used to elucidate the structures of these compounds. We report the unambiguous assignments of the (1)H and (13)C NMR spectra of the new compound nocarsin A (1).

Alkaloids from the Mangrove-Derived Actinomycete Jishengella endophytica 161111

“…J. endophytica 161111 was found to produce alkaloids in a saline culture medium by thin layer chromatography (TLC) visualizing with Dragendorff’s reagent. Chemical investigations on the ethyl acetate (EtOAc) extract of fermentation broth of strain 161111 resulted in the isolation and identification of three pyrazine derivatives, 2-(furan-2-yl)-6-(2 S ,3 S ,4-trihydroxybutyl)pyrazine ( 1 ) and 2-(furan-2-yl)-5-(2 S ,3 S ,4-trihydroxybutyl) pyrazine ( 2 ) [6] and lumichrome ( 10 ) [7]; four pyrrololactones, ( S )-4-isobutyl-3-oxo-3,4-dihydro-1 H -pyrrolo[2,1- c ][1,4]oxazine-6-carbaldehyde ( 3 ) [8], ( S )-4-isopropyl-3-oxo-3,4-dihydro-1 H -pyrrolo[2,1- c ][1,4]oxazine-6-carbaldehyde ( 4 ) [8], (4 S )-4-(2-methylbutyl)-3-oxo-3,4-dihydro-1 H -pyrrolo[2,1- c ][1,4]oxazine-6-carbaldehyde ( 5 ) [8,9] and ( S )-4-benzyl-3-oxo-3,4-dihydro-1 H -pyrrolo[2,1- c ][1,4]oxazine-6-carbaldehyde ( 6 ) [10]; three β-carbolines, flazin ( 7 ) [11], perlolyrine ( 8 ) [12] and 1-hydroxy-β-carboline ( 9 ) [13]; along with 1 H -indole-3-carboxaldehyde ( 11 ) [14,15], 2-hydroxy-1-(1 H -indol-3-yl) ethanone ( 12 ) [14,15], and 5-(methoxymethyl)-1 H -pyrrole-2-carbaldehyde ( 13 ) [16] (Figure 1). Compounds 8 – 11 showed anti-influenza A (H1N1) virus activity with the half maximal inhibitory concentration ( IC 50 ) and selectivity index ( SI ) values of 38.3(±1.2)/25.0(±3.6)/39.7(±5.6)/45.9(±2.1) μg/mL and 3.0/16.1/3.1/11.4, respectively.…”

Antiprotozoal and Antimicrobial Compounds from the Plant Pathogen Septoria pistaciarum

“…8 In this study, the perylenequinones showed antileishmanial, antiplasmodial, and cytotoxic activities in addition to antibacterial and antifungal activities. Two more known compounds, lumichrome, 9 and brassicasterol 10 were also isolated and identified.…”

Natural Products from Diaporthe arecae with Anti‐Angiogenic Activity

“…Finally, a new compound, arecine (1), together with twenty known diketopiperazines, cordysinin A (2), [17] cyclo(l-Thr-l-Pro) (3), [18] cyclo(6-hydroxy-Pro-l-Leu) (4), [19] cyclo(l-Val-l-Pro) (5), [20] bacillusamide B (6), [21] cyclo(l-Leu-l-Pro) (7), [20] cyclo(l-Val-l-Ala) (8), [22] cyclo(l-Leu-l-Ala) (9), [23] cyclo(l-Ile-l-Ala) (10), [22] cyclo(Gly-l-Val) (11), [24] cyclo(Gly-l-Leu) (12), [25] cyclo(Gly-l-Ile) (13), [26] cyclo(l-Ile-d-Pro) (14), [27] staphyloamide A (15), [19] cyclo(l-Ala-l-Pro) (16), [20] cyclo(l-Ser-l-Pro) (17), [28] cyclo(l-Trp-l-Pro) (18), [29] cyclo(l-Tyr-l-Pro) (19), [30] cyclo(l-Phe-l-Ala) (20), [31] and cyclo(l-Ser-l-Phe) (21) [32] were identified. On the other hand, the fungus D. arecae was also cultured by the PDB condition with 10 % NaCl in room temperature for two weeks.…”