SUN-102 Spexin Differentially Regulates Adipogenesis in Brown and White Adipose Tissue Depots

Abstract

In the last decade, neuropeptide Q or spexin (SPX) was implicated in central and peripheral functions including feeding suppression and lipid metabolism. To test whether spexin acts on brown and white adipose tissue, we performed subcutaneous micro-osmotic infusion of spexin in mice with diet-induced obesity. Our preliminary results indicate that spexin may differentially regulate biomarkers of adipogenesis and insulin sensitivity in brown and white adipose tissue. Spexin infusion increased interscapular brown adipose tissue mass and mRNA expression of adiponectin, fatty-acid binding protein 4, lipoprotein lipase, and C/EBP-⍺ in female C57BL/6 mice. Within gonadal white adipose tissue, spexin and PPAR-γ2 mRNA expression was upregulated while immunohistochemistry of gWAT revealed that β3-adrenergic receptor expression was significantly decreased while adipocyte diameter was increased. These results indicate that spexin infusion differentially regulates adipogenesis within brown adipose tissue by increasing BAT mass and increasing non-adrenergic regulation of adipogenesis within gonadal white adipose tissue.