Sumoylation Plays Fundamental Roles During Eye Development and Pathogenesis

Liu, Yizhi; Li, David W

doi:10.2174/156652401808190130160225

Cited by 1 publication

(1 citation statement)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Being a member of the SUMO family, SUMO1 participates in growth, development, and disease in mammals (14)(15)(16). SUMOylation plays fundamental roles during eye development and pathogenesis and involved in multiple cellular processes, such as regulation of transcription, apoptosis, transport to the nucleus, protein stability, cellular stress response and cell cycle progression (17)(18)(19). Recently an increasing number of studies have connected SUMOylation to the pathological changes of eye diseases, including diabetic retinopathy, UVB-induced retinal diseases, retinal dystrophies and so on (20)(21)(22).…”

Section: Introductionmentioning

confidence: 99%

SUMOylation of GMFB regulates the stability and function of GMFB in RPE cells under oxidative stress and inflammation

Sun¹,

Wang²,

Zhang³

et al. 2021

Preprint

View full text Add to dashboard Cite

Glia maturation factor beta (GMFB) is a growth and differentiation factor that act as an intracellular regulator of signal transduction pathways. The SUMOylation is a post-translational modification (PTM) that plays a key role in protein subcellular localization, stability, transcription, and enzymatic activity. Recent studies have highlighted the importance of SUMOylation in the inflammation and progression of numerous diseases. But little is known about the relationship between GMFB and SUMOylation. Here we first report that GMF can be mono-SUMOylated at multiple sites by the covalent addition of a single SUMO1 protein, and identified K20, K35, K58, and K97 as major SUMO acceptor sites. We also found that SUMOylation leading to increased stability and trans-localization of GMF. Furthermore, RNA-seq data and Real-time quantitative polymerase chain reaction (rt-qPCR) also indicated that the SUMOylated GMF upregulated multiple pathways, including the cytokine-cytokin receptor interaction, NOD-like receptor signaling pathway, TNF signaling pathway, RIG-I-like receptor signaling pathway, and NF-kappa B signaling pathway. Our studies intend to provide a novel direction for the study into the biofunction of GMFB, SUMOylated GMFB and the mechanism, clinical therapy, and prognosis of inflammation-related RPE disorders like age-related macular degeneration (AMD) and diabetic retinopathy (DR).

show abstract