Summary of Rotochute Development

Robinson, Jr

doi:10.21236/ad0118733

Cited by 24 publications

(36 citation statements)

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“…Robinson and Lust [3] reported that oxalate did not alter Ca2+ uptake of brain microsomes, while Trotta and de Meis [34] demonstrated its enhancement with a relatively high concentration of oxalate. The lack of oxalate enhancement reported by Robinson and Lust [3] may be due to a short incubation time, since oxalate showed no effect in the first 5 min of incubation (Fig. 2).…”

Section: Discussionmentioning

confidence: 79%

Characterization of ATP-dependent Ca2+ uptake by canine brain microsomes with saponin

Inamitsu

Ohtsuki

1984

Eur J Biochem

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ATP‐dependent Ca2+ uptake by brain microsomes was classified into two fractions according to the sensitivity to saponin. Properties of each fraction of Ca2+ uptake were examined and compared with those of inside‐out membrane vesicles of erythrocyte and cardiac sarcoplasmic reticulum. The concentration of saponin for 50 % inhibition (IC50) of major saponin‐sensitive Ca2+ uptake was 11 μg/ml, and this uptake was enhanced by calmodulin. The minor saponin‐insensitive Ca2+ uptake fraction (IC50;90 μg/ml) was not affected by calmodulin but was enhanced by oxalate or 0.1 M KC1. The IC50 of saponin for inside‐out membrane vesicles of erythrocyte and cardiac sarcoplasmic reticulum was 11.3 and 114.8 μg/ml, respectively. A characteristic ring‐like saponin‐cholesterol micellar structure was observed electron microscopically in most membrane vesicles of brain microsomes and erythrocyte membrane vesicles but not in the cardiac sarcoplasmic reticulum. These observations indicate that saponin‐sensitive and insensitive Ca2+ uptake was derived from plasma membranes and endoplasmic reticulum, respectively. Saponin proved useful for distinguishing the Ca2+ transport activity of plasma membrane from the Ca2+ uptake of other cellular organelles in the membrane preparations.

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Section: Discussionmentioning

confidence: 79%

Characterization of ATP-dependent Ca2+ uptake by canine brain microsomes with saponin

Inamitsu

Ohtsuki

1984

Eur J Biochem

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“…Sex differences in pigs on different planes of nutrition have been observed by McMeekan (194oa, b) and Robinson (1964) and also on deficient dietary protein levels by Robinson &Lewis (1964) andPrescott (1963). The present study involving the use of forty individually fed pigs was undertaken to examine the response of gilts and castrate male pigs to diets supplemented in varying degrees with synthetic preparations of the first and second limiting amino acids.…”

Section: Radio-chromium Sesquioxide As An Intestinal Marker In Sheep mentioning

confidence: 88%

“…Some of these are inherent problems common to all species and some refer to the methods of experimentation and the parameters used to measure efficiency in the pig. In the first category are factors such as the changing requirements during growth (Braude, 1958) the effect of genotype (Bowland & Berg, 1959) the endocrine status of the animal (Prescott, 1963) and the sex (Robinson & Lewis, 1964) as well as considerations such as the non-availability of amino acids. Problems associated with the method of experimentation have only recently been recognized.…”

Section: Radio-chromium Sesquioxide As An Intestinal Marker In Sheep mentioning

confidence: 99%

Abstracts of Communications

1965

Proc. Nutr. Soc.

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The feed intake and performance of Sussex x Ayrshire beef cattle grazing a perennial ryegrass-white clover ley from July to September under restricted and unrestricted conditions, with and without barley supplementation, were measured. Sixteen animals averaging 21 months of age were allocated at random to a 2x2 factorial experiment, each of the four treatments being replicated four times, and three observations were made at monthly intervals. Restricted and unrestricted grazing was achieved by stocking two paddocks at rates of 2.2 and I .I animals/acre, respectively; further, animals on restricted grazing were permitted access to the pasture for only 10 h daily from 8 am to 6 pm whilst for the remainder of the day they were housed. Barley was offered to the supplemented groups at the rate of 0.075 lbjlb live weight0.73. Between 7 and 8 am daily and again between 2.30 and 3.30 pm each animal was dosed with 10 g chromic oxide and received half the barley ration when offered; rectal grab-samples were also taken at these times during estimation of feed intake which took place during two 4-day periods at the end of each month. Feed intakes were estimated by the faecal nitrogen-chromic oxide technique. The animals were weighed on 3 successive days before and after each feed intake estimate. Pasture availability was measured at the beginning of each feed intake estimate by cutting random sample areas of the paddocks. The results are shown in Table I. Grazing pressure determinations showed that about one-quarter as much herbage dry matter was available to the restricted animals as to the unrestricted animals. .. 11Restriction had no effect on the digestibility of the pasture but supplementation improved the overall digestibility of the diet by 2-3 digestibility units; this together with their increased OM intake gave a greatly increased digestible organic matter (DOM) intake in the supplemented groups. The performances of the animals in all treatments did not differ except that the rate of live-weight gain of the unsupplementedrestricted group was significantly ( P = 0.05) lower than that of the other groups.The results showed that grazing restriction with limited barley feeding achieved performances which equalled those of unrestricted grazing; excessive energy intake may have occurred with supplementation of unrestricted grazing. There appear to be several problems in relation to the pig which make it very difficult to establish the necessary basic information on the amino acid needs of the animal. Some of these are inherent problems common to all species and some refer to the methods of experimentation and the parameters used to measure efficiency in the pig. In the first category are factors such as the changing requirements during growth (Braude, 1958) the effect of genotype (Bowland & Berg, 1959) the endocrine status of the animal (Prescott, 1963) and the sex (Robinson & Lewis, 1964) as well as considerations such as the non-availability of amino acids. Problems associated with the method of experimentation have o...

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“…Intra-synaptosomal volume was determined by incubating the synaptosomes for 15 min at 30°C in the standard medium with tracer amounts of 3H-water and 14C-carboxydextran, as previously described (Robinson and Lust, 1968). The difference between the extra-synaptosomal space (**C-carboxydextran space) and the total water space ("-water space) of the pellet was considered the intra-synaptosomal space.…”

Section: Methodsmentioning

confidence: 99%

Calcium accumulation by isolated nerve ending particles from brain. II. Factors influencing calcium movements

Lust

Robinson

1969

J. Neurobiol.

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SUMMARYA synaptosomal preparation from rat brain accumulated W a by a temperature-sensitive process requiring ATPt and Mg + +. The uptake of *Ca was correlated with a net increase in total calcium, and after brief incubations the concentration of "Ca in the particles was several hundred times that remaining in the medium. Calcium accumulation was associated with a corresponding stoichiometric accumulation of phosphate. Synaptosomes that had accumulated "Ca during a prior incubation lost the radioactivity during a subsequent incubation in the absence of ATP. NaCl increased the efflux of accumulated W a , a process distinguishable from the non-specific inhibition of W a accumulation by various solutes corresponding with their effects on intra-synaptosomal volume. The Naf-induced efflux of liCa was not affected by ouabain. Since the intrasynaptosomal mitochondria have been identified as the site of 3 C a accumulation in synaptosomes, the data suggest that these mitochondria may function as reservoirs of releasable Ca++ within the nerve ending, possibly participating in the Ca++-dependent process of neurotransmitter release.

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Summary of Rotochute Development

Cited by 24 publications

References 0 publications

Characterization of ATP-dependent Ca2+ uptake by canine brain microsomes with saponin

Characterization of ATP-dependent Ca2+ uptake by canine brain microsomes with saponin

Abstracts of Communications

Calcium accumulation by isolated nerve ending particles from brain. II. Factors influencing calcium movements

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