It was the objective of this study to compare the suitability of different extractants for predicting the availability of sulfur (S) in natural grassland in a sulfur response trial on three different soil types in the Inner Mongolia steppe of China. For soil analysis, seven different extractants have been employed. The inorganic SO4-S concentration was determined by ion chromatography. Additionally, in the Ca(H:P04)i extract the total soluble S was determined employing turbidimetry. Weak salt solutions (0.15% CaCI., Ca(H2PO4)2, and KH2PO4) extracted similar 721 722 X. Y. Cui et al. amounts of SO4-S. Extraclion with 0.025 M KCl provided the lowest SO4-S values.Deioni/ed water dissolved significanily more SO4-S in Ihe conlrol plots than most weak salt cxtraclaiits. The concentration of soluble organic S decreased in Ihe control plots after 1 (X) days of plant growth, indicating that the organic S pool contrihuted significantly to the S nutrition of the forage crops. Significant relalionships among the SO4-S in the soil determined in different exti'acts and crop yield, sulfur content in the forage, and total sulfur uptake were only found for the Ca(H2PO4)2 extract. In general, the correlation coefficients proved to be unsatisfactory for field experimentation.