Sulphosalicylic acid as spray reagent for the detection of sugars on thin-layer chromatograms

Ray, Bimalendu; Ghosal, Pradyot K.; Thakur, Swapnadip; Majumdar, Sayari

doi:10.1016/s0021-9673(01)90759-9

Cited by 24 publications

(6 citation statements)

References 15 publications

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“…TLC was done on silica gel 60 (E. Merck), using n-butanol-pyridine-water (6:4:3) with detection by anisaldehyde-sulfuric acid and aqueous 2% sulfosalicylic acid. 23 Ascending paper chromatography was performed on Whatman No. 1 and 3 MM paper with the following solvents: A, the upper layer of n-BuOH-EtOHwater (4:1:5); B, EtOAc-pyridine-water (8:4:1); and C, n-BuOH-pyridine-water (6:4:3).…”

Section: Methodsmentioning

confidence: 99%

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Structural Analysis of a Neutral (1→3),(1→4)-β-d-Glucan from the Mycelia of Cordyceps sinensis

2005

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The extracellular polysaccharide (1) extracted from the mycelia of Cordyceps sinensis with hot water was fractionated and purified by ion-exchange and gel-filtration chromatography. The structure was investigated using methylation and hydrolysis analysis, periodate oxidation, NMR spectroscopy, and reaction with beta-D-glucanase, and the results indicated that this D-glucan consisted of a backbone composed of (1-->3)-beta-D-glucosyl residues and carried a single (1-->4)-beta-linked D-glucosyl residue. NMR and IR spectroscopic measurements showed that the sugar residues were beta-glycosidically linked.

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Section: Methodsmentioning

confidence: 99%

“…The ionization potential was 70 eV, and the temperature of the ion source was 220 °C. TLC was done on silica gel 60 (E. Merck), using n -butanol−pyridine−water (6:4:3) with detection by anisaldehyde-sulfuric acid and aqueous 2% sulfosalicylic acid . Ascending paper chromatography was performed on Whatman No.…”

Section: Methodsmentioning

confidence: 99%

Structural Analysis of a Neutral (1→3),(1→4)-β-d-Glucan from the Mycelia of Cordyceps sinensis

2005

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“…Monosaccharides in the acid hydrolysate were also analyzed by thin layer chromatography on kieselgel 60F plate (Merck) using EtOH/PhOH/Pyridine/0.1 M H 3 PO 4 (5/1/1/2 v/v) as eluent. Monosaccharides were then detected by heating at 100 • C after treatment with sulfosalicylic acid [21]. The monosaccharide composition was also determined after methanolysis by GC analysis of the per-O-trimethyl-silylated methyl glycosides as described [22].…”

Section: General Experimental Proceduresmentioning

confidence: 99%

Extracted polysaccharide from Nyctanthes arbor-tristis leaves: Chemical and antitussive properties

Ghosh

Nosálóvá

Ray

et al. 2015

International Journal of Biological Macromolecules

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“…[ 41 ] 5‐sulfosalicylic acid is strong Brønsted acid with numerous applications in different fields. It has been used to determine the protein content in urine, [ 42 ] as a sugar visualizing reagent on TLC, [ 43 ] as a chelating agent, [ 44 ] and as an organocatalyst in organic reactions. [ 45,46 ] 5‐SSA is an organocatalyst that has properties like biodegradability, stability, nontoxicity, water‐solubility, and excellent catalytic activity, which leads us to choose it to catalyze the synthesis of highly derivatized piperidines at room temperature (Scheme 1).…”

Section: Introductionmentioning

confidence: 99%

5‐Sulfosalicylicacid an organocatalyst for the synthesis of highly functionalized piperidines through the multicomponent reaction

Gadge

Kulkarni²

2022

Journal of Heterocyclic Chem

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The synthetic protocol was developed for the synthesis of highly derivatized piperidines molecules in high yield with 5‐sulfosalicylic acid as an organocatalyst via a multicomponent reaction between the aromatic aldehydes, the aromatic anilines, and the β‐ketoesters at room temperature. The method has numerous advantages over other reported methods, such as the use of an inexpensive, reusable, nontoxic, and metal‐free catalyst along with readily available precursors, mild reaction conditions, and simple workup procedures. Instrumental techniques like high‐resolution mass spectroscopy (HRMS), proton nuclear magnetic resonance (1H NMR), 13‐carbon nuclear magnetic resonance (13C NMR), and Fourier transform infrared (FTIR) spectroscopy were used to confirm the structure and relative stereochemistry of the compounds. In this protocol, an anti‐isomer has been obtained as a major product at room temperature. Syn: Anti diastereomeric ratio has been confirmed by 1H NMR.

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Sulphosalicylic acid as spray reagent for the detection of sugars on thin-layer chromatograms

Cited by 24 publications

References 15 publications

Structural Analysis of a Neutral (1→3),(1→4)-β-d-Glucan from the Mycelia of Cordyceps sinensis

Structural Analysis of a Neutral (1→3),(1→4)-β-d-Glucan from the Mycelia of Cordyceps sinensis

Extracted polysaccharide from Nyctanthes arbor-tristis leaves: Chemical and antitussive properties

5‐Sulfosalicylicacid an organocatalyst for the synthesis of highly functionalized piperidines through the multicomponent reaction

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