Sulphate production byParacoccus pantotrophusATCC 35512 from different sulphur substrates: sodium thiosulphate, sulphite and sulphide

Abstract

One of the problems in waste water treatment plants (WWTPs) is the increase in emissions of hydrogen sulphide (H2S), which can cause damage to the health of human populations and ecosystems. To control emissions of this gas, sulphur-oxidizing bacteria can be used to convert H2S to sulphate. In this work, sulphate detection was performed by spectrophotometry, ion chromatography and atomic absorption spectrometry, using Paracoccus pantotrophus ATCC 35512 as a reference strain growing in an inorganic broth supplemented with sodium thiosulphate (Na2S2O3·5H2O), sodium sulphide (Na2S) or sodium sulphite (Na2SO3), separately. The strain was metabolically competent in sulphate production. However, it was only possible to observe significant differences in sulphate production compared to abiotic control when the inorganic medium was supplemented with sodium thiosulphate. The three methods for sulphate detection showed similar patterns, although the chromatographic method was the most sensitive for this study. This strain can be used as a reference for sulphate production in studies with sulphur-oxidizing bacteria originating from environmental samples of WWTPs.

Identification of sulfur-oxidizing bacteria from fishponds and their performance to remove hydrogen sulfide under aquarium conditions

“…A total of 27 isolates were obtained and puri ed from the sediment of the warm-water shpond. Similar to these results, some researchers ( In this study, sodium thiosulfate was used as a source of sulfur for the isolation of SOBs due to its stability at neutral pH, water solubility, and non-toxicity in high concentrations (Luo et al 2013;Meyer et al 2016;Zhoa et al 2016). In agreement with this study, some research (Kantachote et al 2008;Hidayat et al 2017) have used sodium thiosulfate as a sulfur substrate for bacterial growth to isolate hydrogen sul de oxidizing bacteria from the e uent.…”

“…Thymol blue was used as a pH indicator. In this study, sodium thiosulfate was used as a source of sulfur for the SOBs isolation (Luo et al 2013;Meyer et al 2016;Zhoa et al 2016). Table 2 shows the compositions of these media.…”

A new impetus for biodesulfurization: bypassing sulfate inhibition in biocatalyst production

“…Additionally, although the ability to use Na 2 S 2 O 3 and Na 2 SO 3 is not commonly studied, it may also indicate a potential use for gas desulfurization, as evidenced by other authors, 51 however further studies are needed to confirm this potential application.…”

“…Additionally, although the ability to use Na 2 S 2 O 3 and Na 2 SO 3 is not commonly studied, it may also indicate a potential use for gas desulfurization, as evidenced by other authors, 51 however further studies are needed to confirm this potential application. In this study, cells of R. erythropolis strain D1 produced using Na 2 SO 4 as the sole S-source (at 22 mg per L SO 4 2− ), respecting the sulfur : carbon ratio (2.2 mg sulfate g glucose −1 ), were also able to retain BDS activity.…”

Draft Genome Sequence of a Sulfur-Oxidizing Bacterium, Aquamicrobium lusatiense NLF2-7, Isolated from Livestock Wastewater

“…One of the problems in WWTPs is the increase in emission of odor pollutants such as hydrogen sulfide, which can cause damage to the health of human populations and ecosystems. To control emissions of this gas, sulfur-oxidizing bacteria can be used to convert hydrogen sulfide to sulfate ( 7 ). Therefore, in this study, sulfur oxidation bacterial medium ( 8 ) was used for isolation of strain NLF2-7.…”