Sulphasalazine fails to prevent development of mucosal ulceration and 5-lipoxygenase activity in guinea-pigs with chronic inflammatory bowel disease induced by combined bacterial immunization and oral carrageenin

Oestreicher, Paul; Nielsen, Susan T.; Rainsford, K. D.

doi:10.1111/j.2042-7158.1992.tb03662.x

Cited by 1 publication

(1 citation statement)

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“…In addition, colitis was induced with carrageenan (CAR) which is a sulfated polygalactose commonly used as a thickening and stabilizing agent in food and toothpaste (Weiner 1991;WHO Technical Reports Series 1984). Certain modified CARs are able to induce inflammation (Oestreicher et al 1992;Axelsson and Midtvedt 1997). Genuvisco-type CAR, TPH-1 (X-5852) (Copenhagen Pectin, Lille Skensved, Denmark), was used after modification (Axelsson 1996, Axelsson andMidtvedt 1997) by hydrolysis with high NaCl concentration, sulfated by chlorosulfonic acid in formamide, precipitated with ethanol, and freeze-dried.…”

Section: Induction Of Colitismentioning

confidence: 98%

Selective expression of detoxifying glutathione transferases in mouse colon: effect of experimental colitis and the presence of bacteria

Edalat

Mannervik

Axelsson

2004

Histochem Cell Biol

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Glutathione transferases (GSTs) play a central role in the cellular defense against harmful endogenous compounds and xenobiotics in mouse and man. The gastrointestinal channel is constantly exposed to bacteria, bacterial products, and xenobiotics. In the present study the distribution of alpha, mu, and pi class GSTs was examined immunohistologically in the colon of conventional and germ-free (GF) mice subjected to experimental colitis. The tissues samples were from conventional mice with and without colitis induced by dextran sulfate sodium (DSS); GF mice treated with DSS or carrageenan; and GF mice inoculated with normal mouse bacterial flora as well as with Lactobacillus GG. In conventional as well as in GF mice the mu and pi class GSTs showed reduced intestinal expression when colitis was induced. In con-rast, the level of GSTs reacting with antibodies directed against the alpha class, in particular mGST A4-4, was elevated after induction of inflammation. Of special interest is mGST A4-4 because of its high catalytic activity with toxic products of lipid peroxidation. In the colon of conventionalized GF mice that were given mouse intestinal flora, the mGST A4-4 expression was increased with time for several weeks, but then showed a decrease to a normal level. Additionally, the inoculation of GF mice with Lactobacillus GG induced all the intestinal GSTs studied.

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Section: Induction Of Colitismentioning

confidence: 98%