Sulfurisphaera ohwakuensis gen. nov., sp. nov., a novel extremely thermophilic acidophile of the order Sulfolobales

Kurosawa, Norio; Itoh, Yuko; Iwai, Toshie; Sugai, Akihiko; Uda, Ikuko; Kimura, Naohiro; Horiuchi, Tadao; Itoh, Toshihiro

doi:10.1099/00207713-48-2-451

Cited by 110 publications

(49 citation statements)

References 29 publications

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“…It is still unclear whether this reflects geographic isolation. Sulfurisphaera ohwakuensis has been obtained from acidic hot springs in Ohwaku Valley, Hakone, Japan (Kurosawa et al, 1998), while several Sulfurococcus species were isolated from a crater of the Uzon volcano in Kamchatka, Russia, and from a thermal spring in Yellowstone National Park, United States (Golovacheva et al, 1987a;Karavaiko et al, 1994).…”

Section: Metallosphaeramentioning

confidence: 99%

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Sulfolobales

Huber¹,

Prangishvili²

2006

The Prokaryotes

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Section: Metallosphaeramentioning

confidence: 99%

“…Representatives of the genera Acidianus and Sulfurisphaera can both oxidize and reduce molecular sulfur (Segerer et al, 1985;Zillig et al, 1985;Kurosawa et al, 1998). Anaerobic growth occurs via S°-H 2 autotrophy (Fischer et al, 1983) and large amounts of H 2 S are formed (equation 2):…”

Section: Physiologymentioning

confidence: 99%

Sulfolobales

Huber¹,

Prangishvili²

2006

The Prokaryotes

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“…2 and Table 3). Sequences of 37.5% clones in HS03 were affiliated with Sulfolobus tokodaii or Sulfurisphaera ohwakuensis (both shared 91 -97% identity) isolated from acidic springs in Japan, 94.6% in HS04 with Sulfolobus metallicus from Icelandic solfataric fields, and 2.7, 5.4, and 21.6% in LH02 with S. metallicus, S. tokodaii and Acidianus infernus, respectively (Table 3) (Segerer et al 1986;Huber and Stetter 1991;Kurosawa et al 1998;Suzuki et al 2002). Sequences of other archaeal planktonic members were related to either Vulcanisaeta distributa isolated from several geothermal springs in eastern Japan (62.5% in HS03) or Caldisphaera lagunensis from a hot pond in the Philippines (4.6% in HS04 and 10.8% in LH02) (Itoh et al 2002;Itoh et al 2003).…”

Section: Compositions Of Archaeal and Bacterial Communitiesmentioning

confidence: 99%

“…Phylotypes with sequences affiliated with the Order Sulfolobales in the planktonic communities constituted 30 to 95% of the individual archaeal libraries (Table 3). Strains related to the Order Sulfolobales are generally thermophilic, acidophilic, and capable of acquiring metabolic energy from aerobic oxidation of elemental sulfur (Kurosawa et al 1998;Harald and David 2006). Some strains within the Order Sulfolobales could switch between aerobic and anaerobic metabolisms (e.g., Sulfurisphaera and Acidianus).…”

Section: Segregation Of Archaeal Assemblages In Water Columns and Botmentioning

confidence: 99%

Segregated Planktonic and Bottom-Dwelling Archaeal Communities in High-Temperature Acidic/Sulfuric Ponds of the Tatun Volcano Group, Northern Taiwan

Cheng¹,

Wang²,

Song³

et al. 2013

Terr. Atmos. Ocean. Sci.

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Geothermal environments are characterized by dynamic redox and temperature fluctuations inherited from the exposure of deeply-sourced, hot, reducing fluids to low-temperature, oxidizing ambient environments. To investigate whether microbial assemblages shifted in response to the changes of a redox state within acidic hot ponds, we collected three paired water and sediment samples from the Tatun Volcano Group, assessed metabolic roles of community members, and correlated their functional capabilities with geochemical factors along depth. Molecular analyses revealed that Sulfolobus spp., Acidianus spp. and Vulcanisaeta spp. capable of respiring elemental sulfur under oxic and/or low-oxygen conditions were the major archaeal members in planktonic communities. In contrast, obligate anaerobic Caldisphaera spp. dominated over others in bottomdwelling communities. Bacteria were only detected in one locality wherein the majority was affiliated with microaerophilic Hydrogenobaculum spp. Cluster analyses indicated that archaeal communities associated with sediments tended to cluster together and branch off those with water. In addition, the quantities of dissolved oxygen within the water column were substantially less than those in equilibrium with atmospheric oxygen, indicating a net oxygen consumption most likely catalyzed by microbial processes. These lines of evidence suggest that the segregation of planktonic from bottom-dwelling archaeal assemblages could be accounted for by the oxygen affinities inherited in individual archaeal members. Community assemblages in geothermal ecosystems would be often underrepresented without cautious sampling of both water and sediments.

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“…The temperature and pH of the water were approximately 55°C and 8.3, respectively. The sample was plated onto modified Brock's basal salts (Kurosawa 1998) supplemented with 0.1% (w/v) yeast extract and artificial sea salts (1% w/v) at pH 8.3, solidified by 0.7% (w/v) Gelzan (Sigma), and incubated at 50°C for two days. Colonies were picked up and purified by repeated single-colony isolation.…”

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confidence: 99%

Characterization of Moderately Thermophilic Bacteria Isolated from Saline Hot Spring in Japan

Kawasaki

Aoki

Makino

et al. 2011

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Bacillus aeoliusTwelve strains of moderately thermophilic bacteria were isolated from a saline hot spring (55°C, pH 8.3) located in Odaito, Hokkaido, Japan. All strains showed over 99% sequence similarities in the 16S rRNA gene. However, only eight strains grew well at 55°C and demonstrated detectable activities. The representative strains, BEK6 and BEK11, were chosen from individual phenotypic groups and were further characterized. The nucleotide sequence of the almost full-length 16S rRNAgenes of the representative strains showed 97.1% and 96.6% similarity with and , respectively, indicating that the strains belonged to a novel species of the genus . The cells of both strains were Gram-positive and showed catalase and oxidase activities. The optimal growth temperature and pH of strains BEK6 and BEK11 was approximately 50°C and 7-8, respectively. These strains grew in a medium containing 10% NaCl, in contrast to and that grew in a medium containing maximum of 4-5% NaCl. The strain BEK11 showed relatively strong protease and amylase activities in the presence of 3% NaCl, suggesting the potential industrial uses of these enzymes under saline conditions. Key words: thermophile, hot spring, novel species, Dua belas galur bakteri termofilik telah diisolasi dari sumber air panas berkadar garam tinggi (55°C, pH 8.3) yang terletak di Odaito, Hokkaido, Jepang. Semua galur bakteri menunjukkan lebih dari 99% persamaan sekuens dalam gen rRNA 16S. Hanya delapan galur bakteri yang dapat tumbuh dengan baik pada suhu 55°C dan mempunyai aktivitas -galaktosidase. Dari kelompok fenotipe individu BEK6 dan BEK11 dipilih sebagai perwakilan. Urutan nukleotida 16S rRNA secara parsial dari BEK6 dan BEK11 menunjukkan masing-masing 97.1% dan 96.6% kesamaan dengan dan , menunjukkan bahwa galur tersebut adalah spesies baru dari genus . Kedua bakteri adalah Gram-positif dan mempunyai aktivitas katalase dan oksidase. Suhu pertumbuhan optimal galur BEK6 dan BEK11 ialah sekitar 50°C dan pH 7-8. Berbeda dengan dan yang hanya dapat tumbuh dalam medium yang mengandung NaCl maksimum 4-5%, kedua galur bakteri tersebut dapat tumbuh dalam medium yang mengandung NaCl 10%. Galur BEK11 menunjukkan aktivitas protease dan amilase yang yang relatif tinggi dalam kondisi NaCl 3%, sehingga menjanjikan potensinya sebagai produsen enzim industri.Kata kunci: termofil, sumber air panas, spesies baru, β-galactosidase β

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Sulfurisphaera ohwakuensis gen. nov., sp. nov., a novel extremely thermophilic acidophile of the order Sulfolobales

Cited by 110 publications

References 29 publications

Sulfolobales

Sulfolobales

Segregated Planktonic and Bottom-Dwelling Archaeal Communities in High-Temperature Acidic/Sulfuric Ponds of the Tatun Volcano Group, Northern Taiwan

Characterization of Moderately Thermophilic Bacteria Isolated from Saline Hot Spring in Japan

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