Sulfur-Oxidizing Bacteria Dominate in the Water from a Flooded Coal Mine Shaft in Kuzbass

Kadnikov, V. V.; Mardanov, Andrey V.; Beletsky, Alexey V.; Antsiferov, Dmitry V.; Kovalyova, A. A.; Карначук, О. В.; Равин, Н. В.

doi:10.1134/s0026261719010107

Cited by 9 publications

(9 citation statements)

References 18 publications

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“…The remaining OTUs were Methylophilus (0.5-5.9%), Sulfuricurvum (0.6-14.3%), and Haemophilus (1.7-3.8%). All of these have also been found previously in coal mines: Haemophilus [56]; Methylobacter [57]; Methylomonas [58]; Methylophilus [59]; Methylotenera [60]; Sulfuricurvum [61]. The above OTUs corresponded to seven bacterial phyla out of which Proteobacteria dominated all samples (Figure 6).…”

Section: Microbial Diversitysupporting

confidence: 81%

Emerging Ecotone and Microbial Community of a Sulfidic Spring in the Reka River near Škocjanske Jame, Slovenia

et al. 2021

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During long periods with no precipitation, a sulfidic spring (Smrdljivec) appears in the dry bed of the Reka River before sinking into the karst underground. The study characterizes the area’s geological setting, development of microbial communities and an ecotone, and impact on the vulnerable karst ecosystem. Geological mapping of the area, stable isotopic analyses, field measurements, and physico-chemical and toxicity analyses were applied to elucidate the environmental conditions. The spring’s microbial diversity was assessed using cultivation methods, microscopy, and metagenomics. Sulfur compounds in the spring probably originate from coal layers in the vicinity. Metagenomic analyses revealed 175 distinct operational taxonomic units in spring water and biofilms. Proteobacteria predominated in developed biofilms, and a “core” microbiome was represented by methylotrophs, including Methylobacter, Methylomonas, and Methylotenera. Diatoms represented an important component of biofilm biomass. A combination of environmental factors and climatic conditions allows the formation and accessibility of emerging biodiversity hotspots and ecotones. Details of their dynamic nature, global impact, and distribution should be highlighted further and given more protection.

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Section: Microbial Diversitysupporting

confidence: 81%

Emerging Ecotone and Microbial Community of a Sulfidic Spring in the Reka River near Škocjanske Jame, Slovenia

et al. 2021

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“…can anaerobically oxidize pyrite using nitrite as electron acceptor ( 99 ), and may also use hydrogen, sulfur compounds or iron as electron donors ( 100 ). Thiomicrorhabdus have been shown to be versatile sulfur oxidizers in coal mine shafts ( 101 ). In two of the aquifers, they constituted 100 % of the sulfur oxidizers present (Fig S10, GW3026 and GW3004).…”

Section: Supplementary Textmentioning

confidence: 99%

Hydrogen and dark oxygen drive microbial productivity in diverse groundwater ecosystems

Ruff

Humez

Angelis

et al. 2022

Preprint

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Groundwater ecosystems are globally widespread yet still poorly understood. We investigated the age, aqueous geochemistry, and microbiology of 138 groundwater samples from 87 monitoring wells (<250m depth) located in 14 aquifers in the Canadian Prairie. Geochemistry and microbial ecology were tightly linked revealing large-scale aerobic and anaerobic hydrogen, methane, nitrogen, and sulfur cycling carried out by diverse microbial communities. Older groundwaters contained on average more cells (up to 1.4×107/mL) than younger groundwaters. Organic carbon-rich strata featured some of the highest abundances, challenging current estimates of global groundwater population sizes. Substantial concentrations of dissolved oxygen (n=57; 0.52±0.12 mg/L [mean ± SE]; 0.39 mg/L [median]) in older groundwaters could support aerobic lifestyles in subsurface ecosystems at an unprecedented scale. Metagenomics, oxygen isotope analyses and mixing models indicated that microbial ″dark oxygen″ contributed to the dissolved oxygen pool in subsurface ecosystems commonly assumed to be anoxic.

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“…Omnitrophota populations are commonly detected in oligotrophic groundwater environments (Hershey et al, 2018; Kadnikov et al, 2019; Krauze et al, 2017; Němeček et al, 2018) and have been shown to represent up to 34% of the microbial community (Fiedler et al, 2018). This phylum has also been found in permafrost thaw water bodies (Vigneron et al, 2019; Wurzbacher et al, 2017), marine sediments (Haas et al, 2018; Rasigraf et al, 2017; Varliero et al, 2019), mangrove sediments (Ahmad et al, 2019), and lakes (Morrison et al, 2017; Rissanen et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Redefining the phylogenetic and metabolic diversity of phylum Omnitrophota

Perez‐Molphe‐Montoya

Küsel

Overholt

2022

Environmental Microbiology

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The candidate phylum Omnitrophica-recently termed Omnitrophota, and originally known as OP3-is an understudied bacterial clade that has primarily been found in aquatic ecosystems. To characterize the diversity and ecology of this phylum, we reconstructed 55 Omnitrophota metagenomeassembled genomes (MAGs) from a well-characterized groundwater system within central Germany and placed them within the context of publicly available genomes. Seven clades were identified, four of which contained novel genomes obtained from our groundwater system. All clades exhibited the capacity for type IV pili, type II secretion systems, glycogen storage, and carbohydrate degradation. Only the characterized Cand. Omnitrophus magneticus genome exhibited functions associated with magnetosome construction. Clades were characterized by sets of traits rather than unique pathways, which were then used to infer ecological strategies. These lifestyles consisted of mixotrophs, obligate fermenters, and versatile respiratory heterotrophs. Patterns in 16S rRNA gene amplicons from a 6 years, monthly sampled groundwater time-series dataset reflected the persistent and widespread occurrence of Clade 7 Wood-Ljungdahl utilizing mixotrophs and highlight this group as a core member of the groundwater community. Overall, this study uncovered, characterized, and contextualized the metabolic and phylogenetic diversity within phylum Omnitrophota, and predicts that environmental populations may mediate both nitrogen and sulfur cycling, along with organic matter production and degradation within aquatic ecosystems.

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Sulfur-Oxidizing Bacteria Dominate in the Water from a Flooded Coal Mine Shaft in Kuzbass

Cited by 9 publications

References 18 publications

Emerging Ecotone and Microbial Community of a Sulfidic Spring in the Reka River near Škocjanske Jame, Slovenia

Emerging Ecotone and Microbial Community of a Sulfidic Spring in the Reka River near Škocjanske Jame, Slovenia

Hydrogen and dark oxygen drive microbial productivity in diverse groundwater ecosystems

Redefining the phylogenetic and metabolic diversity of phylum Omnitrophota

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