Sulfur cycle contributes to stable autotrophic denitrification and lower N2O accumulation in electrochemically integrated constructed wetlands: Electron transfers patterns and metagenome insights

Wang, Yingmu; Xie, Shikang; Zhou, Jian; Fan, Gongduan; He, Lei; Fan, Xing; Chen, Shi; Yang, Jun; Xu, Junge; He, Qiang

doi:10.1016/j.cej.2022.138658

Cited by 3 publications

(2 citation statements)

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“…Microbial denitrification in estuarine sediments is an effective way to eliminate anthropogenic-derived nitrogen . Prokaryote-mediated denitrification (heterotrophic and autotrophic types − ), as frequently reported, can permanently reduce nitrate (NO 3 – ) and nitrite (NO 2 – ) to nitrous oxide (N 2 O) and dinitrogen (N 2 ), thereby alleviating water eutrophication. Increasing evidence has shown that eukaryote-mediated denitrification might also hold an important role in nitrogen transformations. − Compared with prokaryotic denitrification, it is assumed that this process can emit large amounts of greenhouse gas N 2 O but rarely produces N 2 , , potentially impacting global climate change.…”

Section: Introductionmentioning

confidence: 97%

Denitrification and N₂O Emission in Estuarine Sediments in Response to Ocean Acidification: From Process to Mechanism

Tang

et al. 2022

Environ. Sci. Technol.

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Global estuarine ecosystems are experiencing severe nitrogen pollution and ocean acidification (OA) simultaneously. Sedimentary denitrification is an important way of reactive nitrogen removal but at the same time leads to the emission of large amounts of nitrous oxide (N 2 O), a potent greenhouse gas. It is known that OA in estuarine regions could impact denitrification and N 2 O production; however, the underlying mechanism is still underexplored. Here, sediment incubation and pure culture experiments were conducted to explore the OA impacts on microbial denitrification and the associated N 2 O emissions in estuarine sediments. Under neutral (in situ) conditions, fungal N 2 O emission dominated in the sediment, while the bacterial and fungal sources had a similar role under acidification. This indicated that acidification decreased the sedimentary fungal denitrification and likely inhibited the activity of fungal denitrifiers. To explore molecular mechanisms, a denitrifying fungal strain of Penicillium janthinellum was isolated from the sediments. By using deuterium-labeled single-cell Raman spectroscopy and isobaric tags for relative and absolute quantitation proteomics, we found that acidification inhibited electron transfers in P. janthinellum and downregulated expressions of the proteins related to energy production and conservation. Two collaborative pathways of energy generation in the P. janthinellum were further revealed, that is, aerobic oxidative phosphorylation and TCA cycle and anoxic pyruvate fermentation. This indicated a distinct energy supply strategy from bacterial denitrification. Our study provides insights into fungi-mediated nitrogen cycle in acidifying aquatic ecosystems.

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Section: Introductionmentioning

confidence: 97%

Denitrification and N₂O Emission in Estuarine Sediments in Response to Ocean Acidification: From Process to Mechanism

Tang

et al. 2022

Environ. Sci. Technol.

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“…Compared to MIET using H 2 or formate to transfer electrons, DIET has various advantages, including more rapid electron transfer and the generation of more energy for syntrophic partners [31,32]. MIET and DIET are both important forms of extracellular electron transfer, prevalent across diverse environments (e.g., marine, wetland, and waste treatment facilities) [27,31,33,34], and play a significant role in numerous biogeochemical sulfur cycle processes [35][36][37][38].…”

Section: Introductionmentioning

confidence: 99%

Electron Transfer in the Biogeochemical Sulfur Cycle

Zhuang,

Wang,

2024

Life

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Microorganisms are key players in the global biogeochemical sulfur cycle. Among them, some have garnered particular attention due to their electrical activity and ability to perform extracellular electron transfer. A growing body of research has highlighted their extensive phylogenetic and metabolic diversity, revealing their crucial roles in ecological processes. In this review, we delve into the electron transfer process between sulfate-reducing bacteria and anaerobic alkane-oxidizing archaea, which facilitates growth within syntrophic communities. Furthermore, we review the phenomenon of long-distance electron transfer and potential extracellular electron transfer in multicellular filamentous sulfur-oxidizing bacteria. These bacteria, with their vast application prospects and ecological significance, play a pivotal role in various ecological processes. Subsequently, we discuss the important role of the pili/cytochrome for electron transfer and presented cutting-edge approaches for exploring and studying electroactive microorganisms. This review provides a comprehensive overview of electroactive microorganisms participating in the biogeochemical sulfur cycle. By examining their electron transfer mechanisms, and the potential ecological and applied implications, we offer novel insights into microbial sulfur metabolism, thereby advancing applications in the development of sustainable bioelectronics materials and bioremediation technologies.

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Synergistic effect between biochar and sulfidized nano-sized zero-valent iron enhanced cadmium immobilization in a contaminated paddy soil

Zhou,

Lv,

et al. 2024

Biochar

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Biochar-based sulfidized nano-sized zero-valent iron (SNZVI/BC) can effectively immobilize cadmium (Cd) in contaminated paddy soils. However, the synergistic effects between biochar and SNZVI on Cd immobilization, as well as the underlying mechanisms remain unclear. Herein, a soil microcosm incubation experiment was performed to investigate the immobilization performance of SNZVI/BC towards Cd in the contaminated paddy soil. Results indicated that the addition of SNZVI/BC at a dosage of 3% significantly lessened the concentration of available Cd in the contaminated soil from 14.9 (without addition) to 9.9 mg kg−1 with an immobilization efficiency of 33.3%, indicating a synergistic effect. The sequential extraction results indicated that the proportion of the residual Cd in the contaminated soil increased from 8.1 to 10.3%, manifesting the transformation of the unstable Cd fractions to the steadier specie after application of SNZVI/BC. Also, the addition of SNZVI/BC increased soil pH, organic matter, and dissolved organic carbon, which significantly altered the bacterial community in the soil, enriching the relative abundances of functional microbes (e.g., Bacillus, Clostridium, and Desulfosporosinus). These functional microorganisms further facilitated the generation of ammonium, nitrate, and ferrous iron in the contaminated paddy soil, enhancing nutrients’ availability. The direct interaction between SNZVI/BC and Cd2+, the altered soil physicochemical properties, and the responded bacterial community played important roles in Cd immobilization in the contaminated soil. Overall, the biochar-based SNZVI is a promising candidate for the effective immobilization of Cd and the improvement of nutrients’ availability in the contaminated paddy soil. Graphical Abstract

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Sulfur cycle contributes to stable autotrophic denitrification and lower N2O accumulation in electrochemically integrated constructed wetlands: Electron transfers patterns and metagenome insights

Cited by 3 publications

References 53 publications

Denitrification and N₂O Emission in Estuarine Sediments in Response to Ocean Acidification: From Process to Mechanism

Denitrification and N₂O Emission in Estuarine Sediments in Response to Ocean Acidification: From Process to Mechanism

Electron Transfer in the Biogeochemical Sulfur Cycle

Synergistic effect between biochar and sulfidized nano-sized zero-valent iron enhanced cadmium immobilization in a contaminated paddy soil

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Sulfur cycle contributes to stable autotrophic denitrification and lower N2O accumulation in electrochemically integrated constructed wetlands: Electron transfers patterns and metagenome insights

Cited by 3 publications

References 53 publications

Denitrification and N2O Emission in Estuarine Sediments in Response to Ocean Acidification: From Process to Mechanism

Denitrification and N2O Emission in Estuarine Sediments in Response to Ocean Acidification: From Process to Mechanism

Electron Transfer in the Biogeochemical Sulfur Cycle

Synergistic effect between biochar and sulfidized nano-sized zero-valent iron enhanced cadmium immobilization in a contaminated paddy soil

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Product

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Denitrification and N₂O Emission in Estuarine Sediments in Response to Ocean Acidification: From Process to Mechanism

Denitrification and N₂O Emission in Estuarine Sediments in Response to Ocean Acidification: From Process to Mechanism